ENG/SLO
COLLECTION OF INDUSTRAL MICROORGANISMS (CIM)
INFRASTRUCTURAL CENTRE (MRIC UL)
BIOTECHNICAL FACULTY
JAMNIKARJEVA 101
Si-1000 LJUBLJANA
Head of collection: Prof. Dr. Peter Raspor ()
Curator: Dr. Neža Čadež ()
-Yeast database
-Filamentous fungi database
-Bacteria database
-Price list
History
The collection of industrial microorganisms was systematically organised in 1991 although its beginnings had started after establishment of Agronomical department in 1947. With establishment of Food Science and Technology Department in 1960, the number of strains increased especially because it joined a collection of 150 autochthonous wine strains. All other isolates has been collected during the last 16 years as results of different research projects or represent the type or otherwise interesting strains from other collections.
Objectives:
-Conservation of biotic diversity with collecting of microorganisms isolated from different environments in Slovenia, with an emphasise to collect yeasts, industrially important bacteria and filamentous fungi.
-Maintaining the collected isolates of microorganisms as a genetic resourse for biotechnological applications.
-Taxonomic studies and application of identification techniques for evaluation of dispersial, density and diversity of certian groups of microorganisms.
-Support of pedagogical institutions with microorganisms and education of the ???
Preserving and storage of the cultures
The cultures are stored by two independant methods: in liquid nitrogen and in deep freezers at -80°C.
Database
It contains the taxonomic information (species, genus, author, synonims, name of the person who identified the culture), ecological data (habitat description and geographical location), mode of preservation, date of last manupulation with the strain and other text information of their molecular, phenotypic and biochemical properties of the strain.
Identification and characterization
-Tradicionalna identifikacija kvasovk, ki temelji na mikroskopskem pregledu vegetativnih in spolnih oblik ter na zmožnosti fermentacije sladkorjev ali zmožnosti asimilacije različnih virov ogljika in dušika.
-Sequencing of ribosomal regions (D1/D2 region of large rRNA subunit or ITS regions).
-Restriction analysis with PCR amplified fragments of ribosomal DNA (PCR-RFLP of rDNA), which is a useful method for combining isolates of the same species in the groups which electrophoretic patter can be compared to the type strain or already identified isolates (Raspor et al., 2001). The usefulness of this method was evelauted in taxonomic studies (Smole Možina et al., 1997, Smole Možina et al., 1998, Čadež et al., 2002, Čadež et al., 2003), or in ecological studies Povhe Jemec et al., 2001, Zagorc et al., 2001, Recek et al.,2002, Raspor et al., 2002, Raspor et al., 2006, Cadez et al., 2010).
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-Random amplified polymorphic DNA (RAPD analysis). We use method for grouping of genetically closely related strains into groups (Smole Možina et al., 1998, Čadež et al., 2002, Čadež et al., 2003).
-Electrophoretic karyotyping is a method for separation of intact chromosomes of yeasts by using pulsed-field electrophoresis (Povhe Jemec et al., 2001, Zagorc et al., 2001, Raspor et al., 2002). The methos is especially useful for discrimination between strains of Saccharomyces cerevisiae and for monitoring of population dynamics during wine fermentation.
-Amplified fragment length polymorphism is a recent method for detection of DNA polymorphisms and is based on selective amplification of restriction fragments of genomic DNA. The method was useful for species delimitation (Čadež et al.., 2006).
Services of Collection of industrial microorganisms:
-Dispatch of microorganisms
-Deposit of strains
-Microbiological analysis of the sample
-Species identification
-Molecular typing of strains with various molecular techniques (PCR-RFLP, PCR-RAPD, AFLP, electrophoretic karyotyping).
Funding
The Collection of Industrial Microorganisms is partially financed by Slovenian Research Agency and is a member of the network of infrastructural centres of University of Ljubljana (MRIC UL).