Chapter 5: Peptides and Proteins

Biochemistry Names:

Chapter 5: Peptides and Proteins

4 February 2005

1. Give structures for the following amino acids:

leucine

cysteine

tyrosine

serine

aspartic acid

lysine

2. Describe the “cooperative binding” mechanism of hemoglobin and oxygen. What are the two extreme states of Hb termed?

3. What specific ligands other than oxygen affect hemoglobin’s oxygen-binding properties, and do these ligands increase the tightness of oxygen binding or decrease it? 3 points

a.

b.

c.

4. Describe the principle behind the way gel filtration chromatography works.

5. Describe the principle behind the way affinity chromatography works.

6. What is the solid phase for electrophoresis? What is the basis for the separation of protein molecules in electrophoresis?

7. What is meant by “protein sequencing”? What are two methods used for protein sequencing?

8. Where do the following enzymes cleave any protein? (1 point)

a. carboxypeptidase

b. chymotrypsin

c. trypsin