Developmental Biology

BIO 442

CHAPTER 4 TERMS TO KNOW

The following terms are freely used in your textbook. Make sure you know what they mean, how they are used, and how to use them. When an example is given, make sure you can describe and recall it. If a picture is provided, know what the structure looks like and where it is located. If a diagram describes an experiment, make sure you understand the experiment by being able to describe it in your own words. It is often helpful to look in other chapters of the book to gain a thorough understanding of how the terms are used. Although some terms may not be mentioned in class, as a student of developmental biology you are still responsible for understanding them. Do explore Vade Mecum and the Web sites.

Developmental Biology

BIO 442

Control of heredity

Polyspermy

Sea urchin

Ctenophore

Genomic equivalence

Genome

Metaplasia

Transdifferentiation

Newt eye

Dorsal iris

Ventral iris

Lens

Nuclear potency

Pluirpotency

Totipotency

Clone

Amphibian cloning

Nuclear transplantation

Enucleated egg or oocyte

Activated egg

Donor nucleus

Nuclear transplant embryo

Restricted nuclear potency

Somatic cell

Xenopus laevis

Wild type

Mutant

Mammalian cloning

Dolly

Somatic cell nuclear
transfer

Transgene

Transgenic animal

Differential gene

expression

Polytene chromosome

Chromosome puff

Balbiani ring

Molecular Techniques

All terms in Molecular

Techniques Web site

Nucleic acid hybridization

Reanneal

cDNA

Gel

Gel electrophoresis

Probe

DNA probe

Nitrocellulose

Southern blotting

Southern blot

Autoradiography

Northern blotting

Northern blot

Developmental Northern

Blots

Pax 6 expression

Homozygous

Heterozygous

In situ hybridization

Gene cloning

Plasmid

Vector DNA

Expression vector

Restriction enzyme

DNA ligase

Polymerase chain reaction

PCR

Reverse transcriptase

Oligonucleotide primers

Taq polymerase

Thermostable

Denature

Sequencing

Microinjection

Transfection

Electroporation

Viral transfection

Retroviral vector

Chimera

Hybrid

Chimeric mouse

Embryonic stem cells

Transgenic mouse

production

Homozygous transgenic

mouse

Heterozygous transgenic

mouse

Gene targeting

Gene knockout

Knockout mouse

Bone morphogenetic

protein 7 (BMP7)

Neomycin

Antisense mRNA technique

Sense mRNA

RNA interference

Krüppel gene

Positional gene cloning

Aniridia

PAX6 and Pax6 gene

Candidate gene mapping

Micropthalmia

Mi gene

MITF gene

DNA-binding protein

Waardenburg syndrome

type 2

Preimplantation genetic
diagnosis

DNA microarray

DNA macroarray

Probe

Target

Famous Researchers

Edmund Beecher Wilson

Thomas Hunt Morgan

Theodor Boveri

Nettie Stevens

Robert Briggs

Thomas King

John Gurdon

Ian Wilmut

Ryuzo Yanagimachi