Cell stress and chaperones

Leishmania donovani chaperonin 10 regulates parasite internalization and intracellular survival in human macrophages

Lucie Colineau, Joachim Clos, Kyung-Mee Moon, Leonard J. Foster and Neil E. Reiner*

* Corresponding author:

Department of Medicine, University of British Columbia, Vancouver, BC, Canada.

email:

Supplementary Table 1: Leishmania proteins included in each Gene Ontology term, up-regulated or down-regulated in CPN10+/- Ld as compared to WT Ld.

Biological process / Proteins up-regulated / Proteins down-regulated
biological regulation (GO:0065007) / LmjF.21.1565
LmjF.06.0880
cellular component organization or biogenesis (GO:0071840) / LmjF.32.0230
LmjF.23.1310
LmjF.23.1740 / LmjF.26.1790
LmjF.35.2190
LmjF.15.0810
LmjF.36.0990
LmjF.34.0490
LmjF.33.3090
LmjF.15.1420
LmjF.04.1230
LmjF.22.1560
cellular process (GO:0009987) / LmjF.26.0340
LmjF.32.0230
LmjF.23.1310
LmjF.23.1740
LmjF.30.1050
LmjF.08.0520
LmjF.30.1450
LmjF.17.0600
LmjF.34.0690
LmjF.13.1610 / LmjF.36.3780
LmjF.32.1300
LmjF.21.1565
LmjF.33.3210
LmjF.18.0150
LmjF.31.0250
LmjF.23.0380
LmjF.33.2620
LmjF.06.0880
LmjF.33.2740
LmjF.10.0890
LmjF.04.0710
LmjF.26.1790
LmjF.27.2300
LmjF.35.2190
LmjF.05.0830
LmjF.15.0810
LmjF.06.1070
LmjF.17.0360
LmjF.08.1170
LmjF.26.1900
LmjF.16.0250
LmjF.05.0100
LmjF.36.0310
LmjF.32.0100
LmjF.24.1840
LmjF.36.0990
LmjF.34.0490
LmjF.30.1120
LmjF.16.1350
LmjF.33.3090
LmjF.15.1420
LmjF.34.2510
LmjF.04.1230
LmjF.15.0170
LmjF.35.2090
LmjF.22.1560
localization (GO:0051179) / LmjF.32.0230
LmjF.23.1310
LmjF.23.1740
LmjF.34.0690 / LmjF.19.0985
LmjF.33.3210
LmjF.33.2620
LmjF.26.1900
LmjF.36.0310
LmjF.05.0970
LmjF.16.1180
LmjF.34.0490
LmjF.30.1120
LmjF.19.1600
LmjF.04.1230
LmjF.35.2090
metabolic process (GO:0008152) / LmjF.31.1140
LmjF.26.0340
LmjF.32.0230
LmjF.03.0930
LmjF.25.0170
LmjF.30.1050
LmjF.08.0520
LmjF.19.1540
LmjF.30.1450
LmjF.17.0600
LmjF.13.1610 / LmjF.32.1300
LmjF.19.0985
LmjF.26.0640
LmjF.33.1610
LmjF.21.1565
LmjF.27.0290
LmjF.18.0150
LmjF.31.0250
LmjF.23.0380
LmjF.26.1610
LmjF.06.0880
LmjF.33.2740
LmjF.10.0890
LmjF.04.0710
LmjF.26.1790
LmjF.27.2300
LmjF.35.2190
LmjF.05.0830
LmjF.06.1070
LmjF.17.0360
LmjF.31.0590
LmjF.33.1680
LmjF.18.0820
LmjF.13.0680
LmjF.16.0250
LmjF.05.0100
LmjF.36.0310
LmjF.05.0970
LmjF.11.0470
LmjF.32.0100
LmjF.24.1840
LmjF.05.0180
LmjF.03.0910
LmjF.16.1350
LmjF.20.1010
LmjF.33.3090
LmjF.06.0460
LmjF.15.1420
LmjF.34.2510
LmjF.19.1600
LmjF.15.0050
LmjF.34.3190
LmjF.35.0970
LmjF.15.0170
LmjF.35.2090
LmjF.22.1560
response to stimulus (GO:0050896) / LmjF.21.1565
LmjF.18.0150
LmjF.05.0100
LmjF.05.0970
LmjF.15.1420
Protein class / Proteins up-regulated / Proteins down-regulated
calcium-binding protein (PC00060) / LmjF.30.1050 / LmjF.18.0150
LmjF.10.0890
LmjF.05.0100
chaperone (PC00072) / LmjF.26.0640
LmjF.10.0890
LmjF.04.0710
cytoskeletal protein (PC00085) / LmjF.32.0230
LmjF.23.1310
LmjF.30.1450
LmjF.34.0690
LmjF.13.1610 / LmjF.04.1230
LmjF.35.2090
enzyme modulator (PC00095) / LmjF.32.0230
LmjF.34.0690 / LmjF.33.2740
LmjF.18.0820
LmjF.34.2510
LmjF.15.0170
hydrolase (PC00121) / LmjF.31.1140
LmjF.23.1310
LmjF.03.0930
LmjF.17.0600 / LmjF.33.1610
LmjF.34.3470
LmjF.18.0150
LmjF.33.2740
LmjF.04.0710
LmjF.17.0360
LmjF.13.0680
LmjF.16.0250
LmjF.05.0100
LmjF.24.1840
LmjF.03.0910
LmjF.34.2510
LmjF.15.0170
LmjF.08.1270
isomerase (PC00135) / LmjF.10.0890
LmjF.34.3190
LmjF.35.0970
kinase (PC00137) / LmjF.20.0580 / LmjF.28.1490
LmjF.06.0460
LmjF.36.3780
ligase (PC00142) / LmjF.19.0985
lyase (PC00144) / LmjF.33.1680
LmjF.34.3190
membrane traffic protein (PC00150) / LmjF.23.1740 / LmjF.33.3210
LmjF.16.1180
LmjF.19.1600
nucleic acid binding (PC00171) / LmjF.33.1700
LmjF.25.0170
LmjF.30.1050 / LmjF.31.0250
LmjF.33.2740
LmjF.26.1790
LmjF.27.2300
LmjF.35.2190
LmjF.13.0680
LmjF.11.0470
LmjF.36.0990
LmjF.16.1350
LmjF.33.3090
LmjF.15.1420
LmjF.19.1600
oxidoreductase (PC00176) / LmjF.26.1610
LmjF.06.0880
LmjF.31.0590
LmjF.20.1010
LmjF.15.0050
LmjF.34.3190
phosphatase (PC00181) / LmjF.16.0250
LmjF.34.2510
LmjF.03.0910
LmjF.18.0150
LmjF.15.0170
protease (PC00190) / LmjF.33.1610
LmjF.13.0680
receptor (PC00197) / LmjF.33.2620
transcription factor (PC00218) / LmjF.27.2300
LmjF.13.0680
LmjF.33.3090
transfer/carrier protein (PC00219) / LmjF.30.1050 / LmjF.27.0290
LmjF.26.1900
LmjF.05.0970
LmjF.34.0490
LmjF.30.1120
transferase (PC00220) / LmjF.08.0520
LmjF.19.1540 / LmjF.36.3780
LmjF.06.0880
LmjF.04.0580
LmjF.05.0830
LmjF.05.0970
LmjF.05.0180
LmjF.16.1350
LmjF.06.0460
transporter (PC00227) / LmjF.30.1050
LmjF.17.0600 / LmjF.23.0380
LmjF.34.0490
LmjF.25.0530

Supplementary Table 2:Infected macrophages' proteins included in each Gene Ontology term: up- or down-regulated in macrophages infected with CPN10+/- Ld, as compared to macrophages infected with WT Ld.

Biological process / Proteins up-regulated / Proteins down-regulated
innate immune response / CAPRIN1
CD14
LAIR1
PML
UBA52
UBE2V2
gene expression / DNAJC8
EIF3J
HSPB1
SRSF2
UBA52 / HNRPDL
membrane organization / CHMP5
CLTA
FTL
UBA52
apoptotic process / CD14
PEA15
PML
UBA52
small molecule metabolic process / GGT1
MARCKS
PLIN2
UBA52
regulation of apoptotic process / CSTB
PEA15
UBA52
TGFb receptor signaling pathway / ENG
PML
UBA52
cellular protein metabolic process / EIF3J
TIMM13
UBA52
viral process / CHMP5
PML
UBA52
biological_process / LMNB2
MANF
PROSC / YLPM1
Molecular Function / Proteins up-regulated / Proteins down-regulated
protein binding / CD14
CHMP5
CLTA
CNN2
CNPY2
CSTB
EIF3J
ENG
FAM21C
FTL
GGT1
HSPB1
IK
LAIR1
LIMA1
MAP4
MARCKS
NUDC
PEA15
PLIN2
PML
SRSF2
UBA52
UBE2V2
WIBG / CD33
CSRP1
NDRG1
S100A4
SF3A3
YLPM1
poly(A) RNA binding / CAPRIN1
CSTB
HSPB1
MANF
MAP4
SRSF2
WIBG
ZC3H4 / CSRP1
HNRNPAB
HNRPDL
S100A4
SF3A3
YLPM1
identical protein binding / ARFIP1
FTL
HSPB1
IK
structural molecule activity / CLTA
LMNB2
MAP4
zinc ion binding / LIMA1
PML
TIMM13 / CSRP1
SF3A3
nucleic acid binding / SRSF2 / HNRNPAB
HNRPDL
SF3A3
molecular function / ARFIP1
CLTA
LMNB2