Supplementary Table1: List of genotypes used for polymorphism screening through genic SSRs. Population 1 and 2 consisted of 70 and 52 genotypes with varying grain calcium contents* respectively.
Population 1 (70 genotypes) / Population 2 (52 genotypes)Acc. / [Ca] / Acc. / [Ca] / Acc. / [Ca] / Acc. / [Ca] / Acc. / [Ca]
GE4711 / 393.6 / GE469 / 320.3 / GE4678 / 261.2 / GPHCPB-45 / 452.8 / GPHCPB-5 / 248.1
GE86 / 387 / GE3019 / 316.6 / GE224 / 260.6 / GPHCPB-44 / 439.7 / GPHCPB-15 / 242.4
GE1583 / 380.5 / GE1221 / 313.3 / GE1789 / 260 / GPHCPB-31 / 417.4 / GPHCPB-14 / 238.2
GE4404 / 377.5 / GE1200 / 313.3 / GE218 / 259.8 / GPHCPB-40 / 415.4 / GPHCPB-6 / 237.3
GE619 / 374.6 / GE3219 / 313 / GE3235 / 259.8 / GPHCPB-35 / 392.6 / GPHCPB-32 / 227.9
GE1146 / 370 / GE4004 / 234.7 / GE909 / 253.1 / GPHCPB-26 / 384.8 / GPHCPB-11 / 226.8
GE2136 / 366.8 / GE778 / 309.6 / GE1267 / 253 / GPHCPB-33 / 366.7 / GPHCPB-8 / 224.1
GE4668 / 359.6 / GE591 / 308.1 / GE482 / 251 / GPHCPB-17 / 357.3 / GPHCPB-22 / 219.5
GE2246 / 359 / GE4798 / 302.1 / GE1486 / 251 / GPHCPB-36 / 352.7 / GPHCPB-29 / 201.9
GE1680 / 355.6 / GE3767 / 300.7 / GE3321 / 247.8 / GPHCPB-52 / 330.7 / GPHCPB-12 / 201.2
GE4601 / 347.3 / GE3248 / 300 / GE1093 / 245.8 / GPHCPB-21 / 328.6 / GPHCPB-47 / 197.5
GE4596 / 345.8 / GE4600 / 296 / GE1871 / 245.5 / GPHCPB-37 / 326.0 / GPHCPB-50 / 192.3
GE104 / 345.2 / GE5194 / 295.8 / GE4732 / 241.4 / GPHCPB-42 / 325.9 / GPHCPB-4 / 184.1
GE3885 / 344.7 / GE258 / 292.1 / GE2347 / 239.8 / GPHCPB-20 / 309.4 / GPHCPB-3 / 183.9
GE225 / 343.3 / GE489 / 292 / GE1045 / 238.5 / GPHCPB-24 / 294.8 / GPHCPB-10 / 177.7
GE2447 / 343.2 / GE2238 / 291.6 / GE356 / 237.1 / GPHCPB-48 / 294.0 / GPHCPB-16 / 175.2
GE4471 / 341.1 / GE278 / 288.2 / GPHCPB-39 / 289.1 / GPHCPB-7 / 174.1
GE1376 / 333.8 / GE125 / 285.8 / GPHCPB-19 / 288.1 / GPHCPB-43 / 170.5
GE4806 / 333.1 / GE106 / 283.8 / GPHCPB-41 / 286.2 / GPHCPB-18 / 168.2
GE3149 / 332.4 / GE1064 / 283.1 / GPHCPB-30 / 278.3 / GPHCPB-28 / 165.4
GE123 / 332.3 / GE2624 / 283 / GPHCPB-34 / 274.7 / GPHCPB-9 / 163.9
GE3138 / 330.6 / GE1280 / 278.1 / GPHCPB-46 / 270.5 / GPHCPB-27 / 150.5
GE1163 / 327.3 / GE1621 / 277.3 / GPHCPB-38 / 269.1 / GPHCPB-13 / 146.1
GE4449 / 325.3 / GE3218 / 276.8 / GPHCPB-49 / 257.3 / GPHCPB-2 / 136.1
GE4440 / 324.8 / GE3147 / 270.2 / GPHCPB-51 / 249.2 / GPHCPB-1 / 117.5
GE3764 / 323.5 / GE384 / 269 / GPHCPB-23 / 248.9
GE4547 / 322 / GE1437 / 265.2 / GPHCPB-25 / 248.2
*[Ca] in mg per 100 g seeds
Supplementary Table2: List of genic SSR primers designed from Calcium Transporters (Ca Transporters), Calcium Binding Proteins (CaBPs) And Calcium Regulated Protein Kinases (Ca Kinases) present in rice and sorghum. Type of candidate gene from which the primers were designed, primer code, sequence of the forward and reverse primers (5’-3’), expected product size, respective SSR motif, species from which the primer is designed and the accession/GI number are given.
SNo / Primer code / Primer sequence (5’-3’ Forward and Reverse) / Expected product size / SSR Motif / Species / AccessionGenic-SSR primers from calcium regulated protein kinases
/ CDPK1F / ATGATGAGGCGGGGTACG/ GTCCTCCTTGGTGATGAGCTTC / 329 / (CGC)7 / Rice / >GI|115447974
/ CDPK2F / ATCACGCGGCCAAGAAGT/ CTTCGGGATGGACTTGCAC / 335 / (CCCAAG)3 / Rice / >GI|115454686
/ CDPK3F / GAGGAAGAAACAGCAGCAGG/ TCCGTGCAGAGGTAGGTGA / 240 / (AGCAGA)3 / Rice / >GI|115472864
/ CDPK4F / GAGCAGAAGCAGAAGCAGAAG/ CATTGTCGTCCTCGTAGGTGT / 382 / (AGC)6 / Rice / >GI|115472864
/ CDPK5F / GGCAGTGTTACGCGAGGA/ CTCTCCCCACCTCCACCT / 126 / (GAC)7 / Rice / >GI|115482889
/ CDPK7F / CTCTGCTCCTCCTGCACC/ ATTTTCGTGACCTTGTAATGCC / 336 / ( CGG)5 / Sorghum / >GI|242048269
/ CDPK8F / CATCCAACGCGAAGAAGAAG/ ATCCATGCACAGGTACGTCA / 203 / (CA)6 / Sorghum / >GI|242054876
/ CDPK9F / CTCAAGGACAGCAGGGACAG/ CGCTGAGGAGGATGTAGATGA / 238 / (GCCGCG)3 / Sorghum / >GI|242063619
/ CDPK10F / GGCCAGTGCTGCTCCAAG/ CAGCTCCTTCCCCATCGAGT / 261 / (GGA)7 / Sorghum / >GI|242067332
/ CDPK11F / GAGCCAGCACAGATCACAGAG/ TACTTGTCCTTGAGGTTGTCGG / 281 / (ACG)8 / Sorghum / >GI|242083427
/ CDPK12F / GACCAGTCCTCCCACCAC/ CTAGGTAGGTCACGCCGAAC / 277 / (GCC)6 / Sorghum / >GI|242091518
/ CDPK13F / GCTTTCCTGGTTCTTTGTTGAC/ CCACCTACTTCCTTTTCCTTTCT / 219 / (GCA)6 / Rice / >C22592.1
/ CIPK1F / AAGATCACGCAGAGCAACTACA / TGATGTCAAAGGCGTTCATACT / 355 / (CGA)9 / Sorghum / >GI|229609766
/ CIPK2F / GTTCAAGAGGGGATTCAAGGAG/ ACGCGATGATGTCGAACG / 311 / (CTCCCG)4 / Rice / >GI|256032203
/ CIPK3F / AGCTCGGCTACTTCATGGTG/ ATCCCTTCCTTGTCTTTCTTCC / 265 / (CGACGG)3 / Rice / >GI|37988173
/ CIPK5F / TTCGCCAAGGTCTACTACGC/ AGGTTCTCGGGCTTGAGGT / 398 / (GCA)6 / Sorghum / >GI|229609748
/ CIPK6F / GGAGCTGCACGAGGTGAT/ AGTCCGCCACTTTGAGGTT / 242 / (CG)5 / Sorghum / >GI|229609750
/ CIPK7F / GCCGACGACAAGGAAGAG/ CTCCTCGCTACAGAACGACTG / 351 / (TC)5 / Sorghum / >GI|229609750
/ CIPK8F / GTTACCTCCCCTTCAACGAC/ GAGAAGCTCAGGATGTCGAAC / 331 / (AGG)6 / Sorghum / >GI|229609754
/ CIPK9F / ACGACGAGTGGTTCAAGGTC/ CTACCCTTTCTCGGCTTCTTG / 172 / (GAC)5 / Sorghum / >GI|229609762
/ CIPK10F / CCTTCAGGGCAGGTACGAG/ AGGTCCAGCACGAGGTACAC / 270 / (CG)5 / Sorghum / >GI|229609788
/ CIPK11F / GGAGTGCTTGGTACAGAAAACC/ CTTCCCTGCGACCATACTTATC / 234 / (TC)5 / Sorghum / >GI|229609800
/ CIPK12F / TGTTCAGGAAGATGAGCCG/ GAGAGGGAAATCAGGTGGAAC / 286 / (CCG)6 / Sorghum / >GI|229609776
/ CMK1F / GCATCACCAGGGTTCGCC/ CATCACCAATCCCTCCTTCACC / 371 / (TC)6 / Rice / >LOC_Os05g26870.1
/ CMK2F / AGGACGGCTCGGCCACAG/ AGGGCTCGGTGAAGACGAACTC / 253 / (GGC)5 / Rice / >LOC_Os05g26870.1
/ CMK3F / CGACGGCTACTCGAAGAAGGT/ CTTGCGGATGGCCTTGAC / 136 / (CTCCGC)3 / Rice / >LOC_Os05g26940.1
/ CMK4F / GTTCAAGAGGGGATTCAAGGAG/ ACGCGATGATGTCGAACG / 311 / (CG)5 / Rice / >LOC_Os06g35160.1
/ CMK5F / GGCAGTGTTACGCGAGGA/ CTCTCCCCACCTCCACCT / 126 / (CTCCCG)4 / Rice / >LOC_Os10g36710.1
/ CMK6F / GAGCAGAAGCAGAAGCAGAAG/ CATTGTCGTCCTCGTAGGTGT / 382 / (GAC)7 / Rice / >LOC_Os07g38120.1
/ CMK7F / AAATCAAGAGCCACCAAACCTA/ GAGAAAAGGAGCAACCTCAGAA / 292 / (AGC)6 / Rice / >LOC_Os05g39870.1
/ CMK8F / GGACGCGAGGAAGAAGAAG/ CTCCATGACGAGGTAGATCCTG / 295 / (GAAG)4 / Rice / >LOC_Os05g41090.1
/ CMK9F / GTCATCCTCTTCGTCCTCCTC/ CTTAGGCTCTTGTCGTGTTCG / 377 / (GCG)6 / Rice / >LOC_Os05g41090.1
/ CMK10F / TCAAGCCCGACAACATCC/ GGAGAGGAGCACGTAGAGGAC / 236 / (CGC)5 / Rice / >LOC_Os02g41580.1
/ CMK11F / AGCCCAAGCCGAAGCCTCA/ GGCCTTGTGGGTGACGAGGTAG / 206 / (CG)5 / Rice / >LOC_Os01g43410.1
/ CMK12F / GGTGATAATGATATGCCGGAGT/ CGATCTCCTCCAATTTTGTGAT / 331 / (GCA)6 / Rice / >LOC_Os05g43840.1
/ CMK13F / CACCAAACCTTCTCTTCTCCTC/ TTTTGTCGAATAGCTCTCCTCC / 387 / (CCG)5 / Rice / >LOC_Os07g44290.1
/ CMK14F / ATGATGAGGCGGGGTACG/ GTCCTCCTTGGTGATGAGCTTC / 329 / (GC)7 / Rice / >LOC_Os02g46090.1
/ CMK15F / CGGGAGATACAGATAATGCACC/ AGACGGAGAGGCCGAAGT / 313 / (CGC)7 / Rice / >LOC_Os02g58520.1
/ CMK16F / ACGTCAAGTCGTCTCACTTCC/ ATCCATGCACAGGTACGTCA / 245 / (TCC)5 / Rice / >LOC_Os01g61590.1
/ CMK17F / GTCCGTCCAGTACAAGATCCC/ GTCGTAGTCGTCTTCTTCGTCC / 400 / (CGA)6 / Rice / >LOC_Os04g59450.3
/ CMK19F / CAAGCCGAGCCAAATCCC/ GAGAAGCCGAAGCCCTTGTC / 384 / (GGA)5 / Rice / >LOC_Os07g44710.1
/ CMK20F / GGTGGAGGAGGAGGAGGA/ GTAGAACGGGAACTTGTGCTG / 278 / (CGA)6 / Rice / >gi115453156
/ CK36 / CACCAACGAATACCCTCACC/ AGTGCATCATCCTTGCTTCTTC / 156 / (CT)8 / Rice / >LOC_Os06g03682.1
/ CK1 / GAGTTATCGCATCTCGGCATC/ CCTCCTCCTCCTCCTCAAGAAT / 248 / (CGA)5 / Rice / >LOC_Os08g34240.1
/ CK2 / CCCTTTACCGTTACCATTACCA/ AGATGGGAGGAGAAGGAATTG / 104 / (CT)5 / Rice / >LOC_Os01g61590.1
/ CK3 / CCCTTTACCGTTACCATTACCA/ AATTAGGGTTAGGGTTTGGGG / 141 / (TC)7 / Rice / >LOC_Os06g50030.1
/ CK4 / GTGTGCATCGCCATTAGTAGTC/ AATTTGTTACTCCGCAAACGAG / 283 / (CCA)7 / Rice / >LOC_Os07g33110.2
/ CK5 / TCTTCTCCTACCTTCCTCCTCC/ TCGCTTCAGCCAAGATCC / 279 / (CTC)5 / Rice / >LOC_Os07g38120.1
/ CK6 / CTCATCAATCCAGCCCATC/ AGGAGGAGGGAAAGAGAAAGAA / 137 / (CTG)6 / Rice / >LOC_Os04g35240.1
/ CK7 / AGAGAGAGAGAGAGCAACGGAA/ GACGAAGAAGAAACAAGACGG / 134 / (TC)5 / Rice / >LOC_Os03g25070.1
/ CK8 / TCTCTCTCCTCCTCCTCTCTTG/ TCTTCTTCTTCTTCTTCACCGC / 219 / (AG)9 / Rice / >LOC_Os12g12860.1
/ CK9 / TTCTTCTCCAACTCTCTCCCTG/ CTATGCAAGCACATCTTTCAGC / 314 / (CT)6 / Rice / >LOC_Os12g02200.1
/ CK10 / CGCACCACTGATCCTACAAATA/ CTGGATTCTTCAGGGATTCTTC / 370 / (CT)5 / Rice / >LOC_Os01g10890.1
/ CK11 / CTACTCATCAACCGCACACG/ GAGGGGAATCTCTGATCTAGCA / 248 / (TC)5 / Rice / >LOC_Os04g49510.2
/ CK12 / AGACGAGACGAAGTGGTAGTGG/ CTCTTTTGCCTCCCTCCCT / 144 / (GGA)5 / Rice / >LOC_Os03g41460.1
/ CK13 / TCCTTCTTCTCACTCCTTCTCG/ CAATTCCCTCACAAGGTAGCTC / 210 / (CGG)5 / Rice / >LOC_Os07g48100.1
/ CK14 / CACTCCTCGATCCCATCATC/ CAAAGTACCTCATGCAACTGGA / 352 / (GGCGGG)4 / Rice / >LOC_Os03g03510.2
/ CK15 / TGTCACCACCACACCAACTC/ GTTCTACCTACCTGCGGCTTC / 169 / (AGG)9 / Rice / >LOC_Os03g55600.1
/ CK16 / TCCGTGTATTTAACCACCTTCC/ CTTCTCCTTGTCCATCACCTTG / 286 / (CGC)5 / Rice / >LOC_Os06g35160.1
/ CK17 / AAACCGTATATTGAGGGAGCAA/ ATCAGCATCCCAAAGCTAGAAC / 124 / (GA)15 / Rice / >LOC_Os01g55450.1
/ CK18 / CACCTCCCTCCTCCGATATAA/ ATCATCCTCCTCCTCCTCCTC / 340 / (CGA)5 / Rice / >LOC_Os08g34240.1
/ CK19 / AGTTATCGCATCTCGGCATC/ CCATCTCGTACCTCCCCTG / 323 / (GAG)5 / Rice / >LOC_Os08g34240.1
/ CK20 / AAAGAAAGAGGAGAGAATGGGG/ CAGAGCCAGGACAGCAGTT / 329 / (CG)5 / Rice / >LOC_Os01g59360.1
/ CK21 / GTCCCACCATTTCTTATTACCCT/ AGTCCTTGTCGTACCCGAAGT / 367 / (CT)7 / Rice / >LOC_Os07g22710.1
/ CK22 / AAGCAAGAGAGAAAGCAACCAG/ GTGTGAATTGGGAGAAGAGAA / 106 / (AGGCAG)5 / Rice / >LOC_Os07g44710.1
/ CK23 / AGATCACACAGGAGAGGAGAGG/ GACGAAGAAGAAACAAGACGG / 349 / (AG)17 / Rice / >LOC_Os03g25070.1
/ CK24 / AGAGAGAGAGAGAGCAACGGAA/ GACGAAGAAGAAACAAGACGG / 134 / (TC)5 / Rice / >LOC_Os03g25070.1
/ CK25 / TCTCTCTCCTCCTCCTCTCTTG/ TCTTCTTCTTCTTCTTCACCGC / 219 / (AG)9 / Rice / >LOC_Os12g12860.1
/ CK26 / GTGTTGTTCAATCCAAATCCCT/ GCTGCATTTTCCGATCAAA / 116 / (GA)14 / Rice / >LOC_Os12g07230.1
/ CK27 / CACATTTCTTCCTTCCCTTTTG/ TCGAACCTTTTCTCTCTCTCTCTC / 248 / (GA)11 / Rice / >LOC_Os12g07230.1
/ CK28 / CAGGAGGGAGTTCTATGCAAAC/ CGGAGAAACGGAGAGGAAG / 308 / (CAC)5 / Sorghum / >gi|253761940
/ CK30 / ATCCAGTGTAACTTGCCCAAAC/ GTCAAAGCAGGATGGCTCTAGT / 392 / (GT)5 / Rice / >LOC_Os03g16130.1
/ CK31 / ACCCCTGAATGTAGAATGGAGT/ GTACGTGGCTTTCTTGTGTGTC / 255 / (CT)11 / Rice / >LOC_Os07g06740.2
/ CK32 / ATGTCTGGGCTGGAAAATAAGA/ GACACGATGATAGCGAGAACC / 395 / (AG)5 / Rice / >LOC_Os07g48090.1
/ CK33 / TCGATCAGTTTTGGCATCG/ GGCCGCTAAACAAAGAAGTGTA / 268 / (CA)8 / Rice / >LOC_Os01g59360.1
/ CK34 / ATGATCCCTGTTGTCTCCTGTT/ TTCGTGTACTGAGCTTACTGGG / 192 / (GTA)6 / Rice / >LOC_Os03g41460.1
/ CK35 / TGACTAGGACGCTGCTTGTAAA/ CGGATCGTATCATGTCAAAGTG / 241 / (AT)11 / Sorghum / >gi|253761942
/ KIN1 / GTTTTGGTTTTGGCTTCTGC/AAAATTCCCACCAGGGAGAG / 187 / (AGG)7 / Rice / >LOC_OS06G03682.1
/ KIN2 / ATTTTCAGGGAGCGACAAGAAT/AAGGAATGTGAGTAAACGCCAG / 238 / (TC)8-(C)17 / Rice / >LOC_OS01G18800.1
/ KIN3 / TTGTGCAGCGTATCTTGAATGT/TTCTACGTTCGTTGGGAGGTAT / 397 / (TG)9 / Rice / >LOC_OS03G55600.1
/ KIN4 / GGGTACTGAGAGGGATGTGTGT/AAGGCACTGGGTAGAACCACTA / 286 / (GT)7 / Rice / >LOC_OS03G55600.1
/ KIN5 / TTTTGATCTAGTGCATTGGTGG/CACTCCATTCTACATTCAGGGG / 151 / (CT)9 / Rice / >LOC_OS07G06740.1
/ KIN6 / AAGGCTGTGTTTGTTTGAAGGT/GGGCTGAGTTCTTTTGTTGAAG / 290 / (TA)33 / Rice / >LOC_OS12G07230.1
/ KIN7 / CTAGCAGCCACATAACAAGTGC/TGAATCCACCAGAGAACTCAAA / 219 / (TTG)6 / Sorghum / >GI 242054876
Genic-SSR primers from calcium transporters
/ TRC1 / CAGTGATGACGATGGTGCC/CTTGAACATGAGGAAGCCGTAG / 199 / (GGA)5 / Sorghum / >SB04G008850.1
/ TRC2 / CGACGAGAGCTGCCACGA/CGAGCAGGTAGAAGAGCACGG / 170 / (GCG)5 / Sorghum / >SB01G021270.1
/ TRC3 / GAGACCAAGTTCTACACCGACC/CTCCAGAGCAAGGACAGGAA / 260 / (CAG)6 / Sorghum / >SB01G021270.1
/ TRC4 / TCCAGCTAAAGACTCATCGTCA/CCCAATCAACCAGATCACTGTA / 134 / (AGC)5 / Sorghum / >SB04G010130.1
/ TRC5 / AGGCGACCTCCTAGAGACC/CCTATGAAGCTGAAAACGAACA / 297 / (GAC)6 / Sorghum / >SB04G010130.1
/ TRC6 / AGAGGGGAGAGATGAGGGAG/AGGTTAAGTGACCACAGCCAAT / 322 / (GCG)5 / Rice / >LOC_OS01G48680.1
/ TRC7 / GCGTGGCCGGGTTGTATC/CGAGTGTAGCTTTGGATGGCAG / 139 / (GCG)5 / Rice / >LOC_OS03G42020.1
/ TRC8 / AGTCCGCGTCGTCTTCACTA/TGGTCAATGCAGTAAGTTGGTC / 387 / (GCG)5 / Rice / >LOC_OS04G51610.1
/ TRC9 / GAGGAAGAGGAGAAGAAGGAGG/CTATCAAGCTGAAGGCAAACAC / 244 / (CGT)5 / Rice / >LOC_OS02G21009.1
/ TRC10 / GGACTTGTAGGAGGTGAGCTTG/GGACTTGTAGGAGGTGAGCTTG / 141 / (GAG)5 / Rice / >LOC_OS03G01330.1
/ TRU1 / CATGGAGTTGCAGACGTGATTT/TGCCTGGATGAATCGGCT / 132 / (GTTCA)5 / Sorghum / >SB03G045370.1
/ TRU2 / CTTGCCCACGCCACAAGT/AATTCCCTCTCCCACCAACC / 195 / (AG)14 / Sorghum / >Sb01g043620
/ TRU3 / CCAGTAGCGAACGAAATAGGAG/GACTCTCTGACGGCAACTTCA / 218 / (CCT)5 / Rice / >LOC_OS03G10640.1
/ TRU4 / CAAAAGCAACACAGGGCAT/CGACGAGAAAACCCTAGAAGAG / 138 / (TC)10 / Rice / >LOC_OS04G51610.1
/ TRU5 / AAAGCCTCTTGTCGTCTCTTTG/TCCACTTCAGATCAAATCCACA / 223 / (CCT)5 / Rice / >LOC_OS12G39660.1
/ TRU6 / ACCCACAAGGGTGTGTTCG/CGTAGTGCGTGCGTGTACC / 111 / (CT)7 / Rice / >LOC_OS03G45370.1
/ TRU7 / ACACCCACAAAGAAGAAGAGGA/AAATAGCAACAGCAGCAGCC / 321 / (AGG)5 / Rice / >LOC_OS04G55940.1
/ TRU8 / TGCTTCTTCTTGTGTTGTGCTT/CAAGCTCTAGTTGTTCCTCGCT / 301 / (TC)8 / Rice / >LOC_OS05G51610.1
/ TRU9 / TGGATTGGTTTCTTTCTGCTCT/CAAATGTCAAATGCTCCATGAC / 320 / (CT)8 / Rice / >LOC_OS05G51610.
/ TRI1 / GAACACAACTATGAATTACGTGGC/ACATCACTCCCTCTGTCCCTTA / 370 / (ATA)48 / Sorghum / >SB03G045370.1
/ TRI2 / GCTCGTCGTCATTTTGTTGTC/ATTAGAAAGGGGAAAGGGTGAA / 368 / (GT)6 / Sorghum / >SB06G027770.1
/ TRI3 / GAAATTAAGCTAAGGGGAGGGA/ACAGGGTTTGGTGGTCTGATT / 395 / (GA)7 / Sorghum / >SB04G005130.1
/ TRI4 / CTTTTCTTGGCATACTTCGGAT/ATGTTCAAGGGAGGTGCTAAAC / 369 / (TA)11 / Rice / >LOC_OS03G10640.1
/ TRI5 / AGCTGACCCAAGAGATAAACCA/CTGTGAAGCAATCAAACAAACC / 287 / (CA)8 / Rice / >LOC_OS04G51610.1
/ TRI6 / GTCCTTGCACTCCTGTTTCTTT/AATGTGAGGTGGGATTGAGATT / 366 / (TC)8 / Rice / >LOC_OS01G37690.1
/ TRI7 / TTCAAAGGAAGGGAAGGTATGA/GGTAGGACAATTCACACAGCAG / 292 / (TTA)26 / Rice / >LOC_OS02G04630.1
/ TRI8 / TTTCTCTCTACACGCTTTGCCT/ATCAGGTTCGAGATTGGGTATG / 188 / (CT)18 / Rice / >LOC_OS02G04630.1
/ TRI9 / CAGCGTAAACAAACTCTTGGTG/CAAAGACACGCACACACAGATA / 379 / (AGT)7 / Rice / >LOC_OS02G21009.1
/ TRI10 / CGGATCTCTGTAGGGGTATTGA/AGTGCAAAGGTAAAAGACCTCG / 375 / (AT)5 / Rice / >LOC_OS11G43860.1
Genic SSR primers from calcium binding proteins (CaBPs)
/ CMC2 / AGTTCAGCCGCGTGTTCTC/TGCACTCATCAATGTCCTGGT / 363 / (GAG)8 / Sorghum / >GI|242055612
/ CMC4 / GGAGACGACGATGGCAAG/GGTCGAAGATGGCGAAGAG / 377 / (GCA)5 / Sorghum / >GI|242060315
/ CMC5 / TTCTTCTTCGTCTTCGTCTTCC/CTTGCTCGACACGATCCTC / 262 / (TCG)5 / Rice / >LOC_OS11G04560.1
/ CMC6 / CAGAGGCTACTACCACGACGA/GCTTCTTCCTCACCTGCTTCT / 101 / (CGG)9 / Rice / >LOC_OS10G25010.1
/ CMC7 / CCTCCGCTTCGAGGACTT/AGCTTCTTGAGCACCTCCTG / 204 / (CGG)5 / Rice / >LOC_OS08G04890
/ CMC8 / GCTCCGGTTCGAGGACTT/GAACGCCTCCTTCATCTCC / 124 / (CGG)7 / Rice / >LOC_OS07G48340.1
/ CMC9 / GATGAACGTGGACTGGTCG/ACTTCCTGAACACCCGCTC / 107 / (CGC)6 / Rice / >LOC_OS05G31620.1
/ CMC10 / GACGGAGTCGCTGGAGAG/GCATCATCTGCTTGAACTCG / 380 / (CGG)8 / Rice / >LOC_OS02G39380.1
/ CMC11 / AGACCACGCCCATCCATC/CTCCTTCTTCCCCTTCTCCTTC / 136 / (GCG)5 / Rice / >LOC_OS01G41990.1
/ CMC12 / CAAGCCTGCGGAGACGAC/GCGGTCGAAGATGTTGAAGAG / 379 / (CAG)10 / Rice / >LOC_OS01G32120.1
/ CRC1 / AGATTCCTGATCCAAAGGACAA/TCAATTCCCACATACTGCAAAG / 229 / (GAT)5 / Rice / >LOC_OS05G43170.1
/ CBC1 / CGTTTTCTTCGCCTTCACC/GGAGTTCTTCCTTGTGGATGAG / 311 / (ACC)6 / Sorghum / >GI|229609864
/ CBC2 / GGATATGTGGATTTCGGTTCAC/GCGCCTCTATTTCATTTACGTT / 346 / (TTG)6 / Rice / >LOC_OS12G06510.1
/ CMU3 / GATCAGCGTCCGTCTCTCTAAT/CACAGAACAAAATCAGGACTGC / 342 / (TC)6 / Sorghum / >GB|EU952907
/ CMU4 / CTCATCGTCGTGGGATCG/AAAATGTAGCCCAACTGATTGC / 382 / (GT)6 / Sorghum / >GI|226495606
/ CMU5 / GACAGGTTGCTAGGCTGTTTCT/AATAAGGAGGAGCGTTGAATTG / 208 / (AC)7 / Sorghum / >GI|226507573
/ CMU6 / GTTCTATTGTTGGAAACCCGAA/ACTGGCTGCTATGGGTGGT / 132 / (GCCA)4 / Sorghum / >GI|226528335
/ CMU7 / CCTTCCCTTTTCTTCTCCATTT/GTCTGCATCCTTTGTTTGATGA / 256 / (TCT)5 / Sorghum / >GB|BT061913
/ CMU8 / AGCTGTTGAGAAACGAAAGGAA/CCTGACTCCTGACTCCCGT / 153 / (TC)10 / Sorghum / >GB|BT061913
/ CMU9 / GACACCACACAAATTAAAAGGC/GATTCTCTCGCTCGTCCCT / 116 / (GAGAA)6 / Sorghum / >SB02G043140
/ CMU10 / TTGTAATGACTTCGTCTCCCCT/TGTAGAAGGAAGCAGATCCACA / 264 / (GT)8 / Sorghum / SB07G001680.1
/ CMU11 / TGCAGTCCAATCACTTTCCATA/GCGTCCATATCTCCTCTCCTC / 376 / (GA)18 / Rice / >LOC_Os08g04890.1
/ CMU12 / TCTTCAATTCCTCCACTCGTTT/CTCACCCTCACCTCCACCT / 236 / (TCT)7 / Rice / >LOC_Os12g41110.1
/ CMU13 / AGAGTCAAAGTGAACCATTCCG/GATTCGGTGGGGATCTCG / 398 / (CTCCAT)4 / Rice / >LOC_OS08G02420.1
/ CMU14 / AAACAGGGGTAGAGAGTACGCA/CTTGTCGAAGAGGCTGAAGG / 285 / (TC)7- (C)19 / Rice / >LOC_Os01g16240.1
/ CMU15 / ATTTCTTCCCTTCTCCTCATCC/CCTCCAATTTCCAATCTACCAA / 193 / (ATCC)4 / Rice / >LOC_OS01G72100.1
/ CBU1 / CGAAGAGAGGAAGAAACGCAG/GATGGGATTGTGGGAATGG / 188 / (CGC)5 / Sorghum / >SB08G020190.1
/ CBU2 / ACATATACGGGCTTGCTTGTCT/CCCTCTCTCTCAAGACGGATTA / 245 / (ATGC)4 / Sorghum / >GI|226507235
/ CBU3 / GCGGCTTGATCTGACGAC/TATGGAGCATGTGTGTTCTTCC / 318 / (TTG)6 / Sorghum / >GI|226507235
/ CBU4 / GAACAACGGAAAACACACACAA/CTCTCTTACTCACCTACCCGCC / 358 / (GCT)5 / Rice / >LOC_OS03G42840
/ CBI1 / TGAGACGATGATTCCAAAAGAG/CTCTCTCTCTCGCTGTCGCT / 215 / (GGC)5 / Rice / >LOC_OS02G27940
/ CBI2 / ATGTAGTTGGTTTTGGTGAGGG/GACAGGGAGCTTGAGGAGC / 290 / (GCC)5 / Rice / >LOC_OS10G41510.1
/ CBI3 / CTCAACCTCTACCTCCGCC/TAAACATCTCTTGTTCCACCCC / 351 / (TGC)5 / Rice / >LOC_OS10G41510.1