C383 Study Guide for Exam 2
Spring 2017
Basic Information
- Exam 2: Thursday, March 9, 7:15-9:15PM in Ballantine 013
- Arrive early for assigned seats
- Bring your student ID. Failure to do so will result in getting your exam back later.
- You may use a NON-PROGRAMMABLE calculator.
- All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
Exam Content: The exam will cover chapters 7-15. All material covered in class, homework, and discussion sections could be on the exam. Some major topics include:
Michaelis-Menton kinetics, saturation curves, Km, kcat, turnover number, kcat/Km, catalytic efficiency, Lineweaver-Burk plots, allosteric enzyme, cooperativity, tense/relaxed, sigmoidal curves, feedback inhibition, temperature and pH effect on enzyme rates, irreversible inhibition, group-specific reagents, affinity reagents, transition state inhibitors, competitive inhibitors, uncompetitve inhibitors, noncompetitive inhibitors, mixed inhibitors, penicillin, chymotrypsin mechanism, catalytic triad, oxyanion hole, hemoglobin structure, myoglobin, oxygen binding curves, 2,3-BPG, effect of proton and carbon dioxide on hemoglobin oxygen binding, sickle cell anemia, monosaccharide structure, stereochemistry, epimers, anomers, cyclic monosaccharide structures, disaccharide and polysaccharide, glycoprotein functions and pathologies, fatty acid and triacylglyceride structure, phospholipid, glycolipid, cholesterol, lipid bilayer, membrane fluidity, integral protein structure, anchored proteins, transverse diffusion, sodium/potassium pump, passive transport, active transport, secondary active transport, structural basis of ion channel selectivity, ligand/receptor binding, receptor tyrosine kinase, transducer, G-protein, second messenger, cAMP, kinase cascade, deactivation of signal transduction, Ras, constitutive activity, digestion, zymogens, hydrolysis of macromolecules, digestive roles of organs, protease, lipase, amylase, role of transporters in intestinal cells, chylomicrons, qualitative and quantitative free energy, coupled reactions, phosphoryl group transfer potential, activated carriers
Exam Format: The exam has 110 points, and is out of 100 points. The first 60 points will be multiple choice, short answer, fill in the blank type questions. There will be five 10-point problems, conceptual and/or quantitative problems on the following topics:
- Enzyme kinetics and inhibition (discussion sheet 5, chapters 7-8)
- Chymotrypsin mechanism (chapter 8)
- Hemoglobin (chapter 9)
- Carbohydrate structure (discussion sheet 6, chapter 10)
- Thermodynamics (discussion sheet 7, chapter 15)
Thermodynamic equations and constants will be given.