Biological Invasions (2012)
ONLINE SUPPLEMENTARY MATERIAL
With a little help from my friends: hybrid fertility of exotic Populus x canadensis enhanced by related native Populus nigra
An Vanden-Broeck1, Karen Cox1, Boudewijn Michiels1, Pieter Verschelde1, Marc Villar 2
(1) Research Institute for Nature and Forest (INBO), Gaverstraat 4, B-9506 Geraardsbergen, Belgium (2) INRA - Centre d'Orleans Unite Amélioration, Génétique et Physiologie Forestières, 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, F-45075 Orléans Cedex 2, France
Corresponding author:
Online Resource 1 Parental genotypes of Populus x canadensis and Populus nigra used in the controlled, polymix crosses. DN: Populus x canadensis; N: Populus nigra
Sex / Species / GenotypeCode / Genotype / Origin
MALE / DN / DNM1 / Isières / P. deltoides S77-6 (Iowa) x P. nigra Isières
DNM2 / S682-111 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra Ghoy
DNM3 / S197-b x Horrues/18 / P. deltoides Nordfk County (Ontario) x P. nigra Horrues
DNM4 / S329-31 x GH/4 / P. deltoides Ohio x P. nigra Gibecq - Hellebecq
DNM5 / S329-31 x GH/7 / P. deltoides Ohio x P. nigra Gibecq - Hellebecq
DNM6 / S4-289 - Voorst/2 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra Voorst
DNM7 / S4-38 x Ogy/13 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra Ogy
DNM8 / S812-8 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra S.P. Kapelle
DNM9 / S814-3 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra Ollignies
DNM10 / S831-10 / P. deltoides S77-6 (Iowa) x P. nigra V497 (Toulouse, France)
DNM11 / S4-41 x Mainvault 4/7 / P. deltoides S9-2 (V5 Iowa x V1 Ontario) x P. nigra Mainvault
N / NM1 / Elst / natural setting, Elst, Belgium
NM2 / Horrues2 / natural setting, Horrues, Belgium
NM3 / Horrues3 / natural setting, Horrues, Belgium
NM4 / Lens / natural setting, Lens, Belgium
NM5 / Mainvault / natural setting, Mainvault, Belgium
NM6 / Ollignies / natural setting, Ollignies, Belgium
NM7 / Ogy / natural setting, Ogy, Belgium
NM8 / Pap4 x Ogy / P. nigra Papignies x P. nigra Ogy
NM9 / Remincourt / natural setting, Remincourt, Belgium
NM10 / Terwolde x Isieres / P. nigra Terwolde x P. nigra Isières
NM11 / V497 / Natural setting,Toulouse, France
NM12 / Viaene / natural setting, Viaene, Belgium
FEMALE / DN / DNF1 / Ghoy / P. deltoides S9-2 x P. nigra Ghoy
DNF2 / Oudenberg / P. deltoides S513-60 x P. nigra S157-3
DNF3 / S4-15 x Ostiches/4 / P. deltoides S4-15 x P. nigra Ostiches
DNF4 / S329-31xOgy/6 / P. deltoides S329-31 x P. nigra Ogy
DNF5 / S77-6 / P. deltoides Iowa x P. nigra V497
DNF6 / S4-18 x Gib/10 / P. deltoides S4 x P. nigra Gibecq
DNF7 / S831-7 / P. deltoides S77-6 x P. nigra V497
DNF8 / Vesten / P. deltoides S513-60 x P. nigra S157-4
N / NF1 / Grandmetz x Gib/12 / P. nigra Granmetz x P. nigra Gibecq
NF2 / Lessines-Wann / natural setting, Lessines, Belgium
NF3 / Leten / natural setting, Leten, Belgium
NF4 / Lo-Reninge / natural setting, Lo-Reninge, Belgium
NF5 / Oosterzele / natural setting, Oosterzele, Belgium
NF6 / Ostiches / natural setting, Ostiches, Belgium
NF7 / Poperinge3 / natural setting, Poperinge, Belgium
NF8 / Vleteren29 / natural setting, Vleteren, Belgium
NF9 / Woesten1 / natural setting, Woesten, Belgium
Online Resource 2 Paternal genotypes used within each year for the polymix construction in the controlled crosses and their mean in vitro pollen germination percentage. NA: not assessed
Year of the crossMale Species / Male
Genotype / 2001 / 2002 / 2005 / 2006 / 2007 / 2008
N / NM1 / NA / 100 / 100 / 74
NM2 / 98 / 66
NM3 / 76
NM4 / 17
NM5 / 11
NM6 / 27
NM7 / NA / 54
NM8 / 56
NM9 / 96 / 95
NM10 / NA / 70
NM11 / NA / 55
NM12 / 100
DN / DNM1 / 40
DNM2 / 20
DNM3 / 12
DNM4 / 12
DNM5 / 32
DNM6 / 26
DNM7 / 18
DNM8 / NA / 54
DNM9 / NA / 32 / 32
DNM10 / 85
DNM11 / 32