Supporting Materials

for

Identification of Triclosan-degrading Bacteria in a Triclosan Enrichment Culture using Stable Isotope Probing

Authors: Do Gyun Lee a, Kun-Ching Cho a, and Kung-Hui Chu a*

a Zachry Department of Civil Engineering,

Texas A&M University, College Station, TX 77843-3136, United States

Supporting Information: 4 pages, 1 figure, 1 table

Fig. S1. Separation of the 12C- and 13C-DNA of reference bacteria (E.coli) and triclosan-degrading microcosms.

Table S1. Comparison of observed T-RFs to the T-RFs of known wastewater microorganisms

Wastewater microorganims1,2 / Predicted
T-RF size (bp)3
foam-causing bacteria / Gordona amarae / 84
filamentous bacteria / Eikelboom Type 1863
Leptothrix cholodnii
Leptothrix mobilis
Leptothrix discophora Sphaerotilus natans Lactosphaera pasteurii / 84, 99, 104, 106
nitrifying-denitrifying bacteria / Comamonas denitrificans Pseudomonas stutzeri Nitrosomonas europaea
Hyphomicrobium zavarzinii
Nitrobacter winogradskyi
Nitrosomonas marina Nitrosomonas eutropha / 104, 106
phosphorus-accumulating bacteria / Rhodocyclus sp. strain R6
Tetrasphaera australienesis Tetrasphaera elongata / 104, 106, 108
triclosan-degrading bacteria / Sphingomonas sp. PH-07
Sphingomonas sp. Rd1
Sphingopyxis strain KCY1 Nitrosomonas europaea / 104, 106

1 Common wastewater microorganims cited from previous studies (Yu et al. 2005; Yan et al. 2007).

2 Known triclosan degrading bacteria cited from previous studies (Kim et al. 2011; Roh et al. 2009; Hay et al. 2001; Lee et al. 2012).

3 The predicted T-RFs (in silico) digested with MspI restriction enzyme with the assumption that T-RFs measured by the real-time-t-RFLP are typically 0-3 bases shorter than the predicted T-RFs (Yu et al. 2005).

References

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Kim YM, Murugesan K, Schmidt S, Bokare V, Jeon JR, Kim EJ, Chang YS (2011) Triclosan susceptibility and co-metabolism - a comparison for three aerobic pollutant-degrading bacteria. Bioresour Technol 102:2206-2212

Lee DG, Zhao F, Rezenom YH, Russell DH, Chu KH (2012) Biodegradation of triclosan by a wastewater microorganism. Water Res 46:4226-4234

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