The effect of temperature and pH gradients on Lactobacillus rhamnosus GG gene expression of stress related genes

Journal: Bioprocess and Biosystems Engineering

Authors: JanneWallenius, TuomasUuksulainen, KalleSalonen, JariRautio and TeroEerikäinen

Corresponding author: Janne Wallenius Aalto University, School of Science and Technology, Laboratory of Bioprocess Engineering, P.O. Box 6100, FIN-02015, Finland,

Online Resource 1

Gene expression results from different experiments in scale-down experiments. Values correspond the capillary electrophoresis peak area in TRAC method. Values colored with red were considered as outliers in model forming. Presented values are normalized with internal control but not with ldhD expression values.

ldhD / pstS / atpA / hrcA
Experiment / Replicate 1 / Replicate 2 / Replicate 3 / Replicate 1 / Replicate 2 / Replicate 3 / Replicate 1 / Replicate 2 / Replicate 3 / Replicate 1 / Replicate 2 / Replicate 3
1 / 677 / 1182 / 963 / 1346 / 1657 / 1339 / 16639 / 9512 / 8941 / 4229 / 4247 / 3901
2 / 954 / 1120 / 1024 / 1064 / 1177 / 1086 / 9771 / 10206 / 8820 / 5183 / 6077 / 5573
3 / 974 / 890 / 708 / 1358 / 1316 / 1006 / 8802 / 7522 / 6774 / 6372 / 4708 / 4395
4 / 541 / 709 / 1064 / 901 / 1185 / 1897 / 4285 / 6779 / 13805 / 2413 / 4119 / 6169
5 / 563 / 1035 / 950 / 1318 / 2588 / 2274 / 5258 / 9154 / 8399 / 4305 / 6997 / 6214
6 / 999 / 881 / 883 / 1297 / 1143 / 1013 / 12407 / 9480 / 10545 / 5635 / 4594 / 4715
7 / 1524 / 1785 / 2070 / 2576 / 2958 / 3246 / 11449 / 12399 / 13505 / 5544 / 6411 / 7985
8 / 2933 / 3177 / 2701 / 3085 / 3296 / 2825 / 20444 / 23729 / 19559 / 9742 / 9800 / 8892
9 / 1416 / 1158 / 1414 / 2710 / 1994 / 2859 / 17737 / 13404 / 14703 / 7700 / 5613 / 7345
10 / 1712 / 907 / 945 / 2469 / 1371 / 1453 / 17769 / 9063 / 10321 / 10798 / 6084 / 6023
11 / 1138 / 881 / 1019 / 2064 / 1863 / 1788 / 11756 / 11595 / 10907 / 7474 / 7003 / 6279
aldB / cfa / groEL
Experiment / Replicate 1 / Replicate 2 / Replicate 3 / Replicate 1 / Replicate 2 / Replicate 3 / Replicate 1 / Replicate 2 / Replicate 3
1 / 1777 / 1578 / 1536 / 2014 / 1909 / 1764 / 11006 / 11303 / 11600
2 / 1958 / 2095 / 1986 / 1686 / 1863 / 1617 / 14236 / 17077 / 19054
3 / 2107 / 1862 / 1509 / 1585 / 1640 / 1192 / 18888 / 12743 / 12163
4 / 1043 / 1641 / 2389 / 867 / 1317 / 1585 / 6476 / 11095 / 16073
5 / 1427 / 2383 / 2137 / 1145 / 1810 / 1690 / 12466 / 20685 / 17713
6 / 2632 / 2235 / 2188 / 1947 / 1677 / 1656 / 18292 / 14285 / 14982
7 / 2958 / 3544 / 3650 / 2478 / 2781 / 3258 / 16528 / 18766 / 23570
8 / 4650 / 5020 / 4045 / 5036 / 4825 / 4623 / 30998 / 28130 / 25465
9 / 2730 / 2232 / 2668 / 2278 / 1821 / 2307 / 24791 / 18426 / 25431
10 / 3710 / 2109 / 2185 / 2133 / 1246 / 1320 / 33129 / 18625 / 17569
11 / 2695 / 2225 / 2162 / 1503 / 1242 / 1449 / 22692 / 21317 / 17058

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