Association between KCNJ11 gene polymorphisms and risk of type 2 diabetes mellitus in East Asian populations: a meta-analysis in 42, 573 individuals

Molecular Biology Reports

Lijuan Yang • Xianghai Zhou • Yingying Luo • Xiuqin Sun • Yong Tang • Wulan Guo • Xueyao Han • Linong Ji

Corresponding author: Linong Ji;

Department of Endocrinology and Metabolism, Peking University People’s Hospital,

No. 11 Xi Zhimen Nan Da Jie Main Street, Xi Cheng District,100044Beijing, China

E-mail address: .

Supplementary Table 1 SNPs examining the association of KCNJ11 and T2DM included in the qualitative analysis (n=20)

Study / Genotyping methods / HWE
testing / rs5210 / rs5213 / rs5215 / rs5218 / rs5219 / rs886288 / rs2285676
B.K. Koo, et al. / TaqMan / In HWE / √ / √ / √ / √ / √
Yuichiro Yamada, et al. / Fluorescence-labeled oligonucleotide primers-PCR / In HWE / √ / √
Norihide Yokoi, et al. / MassARRAY system / In HWE / √
Yukiko Sakamoto, et al. / TaqMan / In HWE / √ / √ / √
Yasufumi Doi, et al. / TaqMan / In HWE / √
M. Horikoshi, et al. / TaqMan / In HWE / √
Shintaro Omori, et al. / TaqMan, multiplex-PCR invader assay / In HWE / √
Yasuharu Tabara, et al. / TaqMan / In HWE / √
Fumihiko Takeuchi, et al. / HumanHap550, iPLEX, GoldenGate and TaqMan / In HWE / √
Kazuaki Miyake, et al. / Multiplex PCR-based Invader assay, TaqMan,SSP-PCR-FCSand MassARRAY system. / In HWE / √

SupplementaryTable 1 continued

Study / Genotyping methods / HWE
testing / rs5210 / rs5213 / rs5215 / rs5218 / rs5219 / rs886288 / rs2285676
Zhang XF, et al. / PCR-RFLP / NSa / √ / √
Liu Z, et al. / PCR-sequencing, tetra-primer ARMS-PCR-MADGE system / In HWE / √ / √ / √ / √ / √ / √
Zhou Q, et al. / PCR-RFLP / P<0.05 / √
Peng SB, et al. / PCR-RFLP / In HWE / √
Zhang YF, et al. / PCR-RFLP / In HWE / √
Zhou DZ, et al. / TaqMan / In HWE / √
Wang F, et al. / ABI SNaPshot Multiplex System / In HWE / √
Hu C, et al. / MassARRAY iPLEX system / In HWE / √
Wen J, et al. / iPLEX and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry / In HWE / √
Yu M, et al. / PCR-RFLP / P>0.05 / √

TaqMan: TaqMan SNP Genotyping Assays; PCR-RFLP: PCR-restriction fragment-length polymorphism; HWE: Hardy-Weinberg equilibrium; NS: not stated

a Without data from the article for HWE test

SupplementaryTable 2The distribution of rs5210, rs5215, rs5218 and rs5219 variants in KCNJ11 gene for cases and controls included in the quantitative analysis (n=16)

Study and ethnicity / M/m / R/r / Distribution of genotype / Frequency of allele
Cases / Controls / Cases / Controls
rs5210 / T/C / C/T / C/C / C/T / T/T / C/C / C/T / T/T / C / T / C / T
B.K. Koo,et al.(Korean) / 236 / 365 / 157 / 163 / 286 / 179 / 837 / 679 / 612 / 644
Yukiko Sakamoto,et al.(Japanese) / 213 / 471 / 213 / 188 / 445 / 253 / 897 / 897 / 821 / 951
rs5215 / T/C
I/V / C/T
V/I / V/V / V/I / I/I / V/V / V/I / I/I / V / I / V / I
B.K. Koo,et al.(Korean) / 144 / 372 / 245 / 101 / 259 / 251 / 660 / 862 / 461 / 761
Yuichiro Yamada,et al.(Japanese) / 16 / 48 / 39 / 11 / 28 / 34 / 80 / 126 / 50 / 96
M. Horikoshi,et al.(Japanese) / 131 / 393 / 334 / 113 / 417 / 332 / 655 / 1061 / 643 / 1081
rs5218 / C/T / C/T / C/C / C/T / T/T / C/C / C/T / T/T / C / T / C / T
B.K. Koo,et al.(Korean) / 281 / 356 / 122 / 206 / 292 / 131 / 918 / 600 / 704 / 554
Yukiko Sakamoto,et al.(Japanese) / 255 / 464 / 181 / 237 / 454 / 199 / 974 / 826 / 928 / 852
rs5219 / C/T
E/K / T/C
K/E / K/K / K/E / E/E / K/K / K/E / E/E / K / E / K / E
B.K. Koo,et al.(Korean) / 150 / 364 / 244 / 102 / 273 / 255 / 664 / 852 / 477 / 783
Yuichiro Yamada,et al.(Japanese) / 16 / 48 / 39 / 11 / 28 / 34 / 80 / 126 / 50 / 96

SupplementaryTable 2 continued

Study and ethnicity / M/m / R/r / Distribution of genotype / Frequency of allele
Cases / Controls / Cases / Controls
rs5219 / C/T
E/K / T/C
K/E / K/K / K/E / E/E / K/K / K/E / E/E / K / E / K / E
Norihide Yokoi,et al.(Japanese) / 246 / 734 / 610 / 171 / 570 / 503
Yukiko Sakamoto,et al.(Japanese) / 127 / 446 / 333 / 107 / 396 / 386 / 700 / 1112 / 610 / 1168
Yasufumi Doi,et al.(Japanese) / 85 / 263 / 202 / 161 / 655 / 617 / 433 / 667 / 977 / 1889
Shintaro Omori,et al.(Japanese) / 259 / 763 / 613 / 118 / 512 / 423 / 1281 / 1989 / 748 / 1358
Yasuharu Tabara,et al.(Japanese) / 83 / 232 / 169 / 50 / 195 / 152 / 398 / 570 / 295 / 499
Fumihiko Takeuchi,et al.(Japanese) / 768 / 2511 / 2182 / 890 / 3121 / 2883 / 4047 / 6875 / 4901 / 8887
Kazuaki Miyake,et al.(Japanese) / 1813 / 3035 / 1726 / 3122
Liu Z,et al.(China) / 432 / 572 / 381 / 621
Zhou DZ,et al.(China) / 329 / 863 / 656 / 288 / 930 / 692 / 1521 / 2175 / 1506 / 2314
Wang F,et al.(China) / 87 / 189 / 120 / 57 / 174 / 156 / 363 / 429 / 288 / 486
Hu C,et al.(China) / 1572 / 2126 / 1407 / 2163
Wen J,et al.(China) / 183 / 587 / 395 / 194 / 517 / 425 / 953 / 1377 / 905 / 1367
Yu M,et al.(China) / 27 / 150 / 118 / 22 / 79 / 87 / 204 / 386 / 123 / 253

M/m: major allele/minor allele; R/r: risk allele/reference allele

SupplementaryTable 3 Analysis results of the relationship between rs5215 and rs5219 of KCNJ11 gene and T2DM with OR(95%CI) adjusted for covariates

Genetic contrast / Studies
(n) / Meta-analysis
model / OR(95%CI) / P(Z)a / Heterogeneity test / Test of publication bias
I2 / P / Begg's test / Egger’s test
z / P / t / P
rs5215
Additive model / 2 / Fixed / 1.108(0.993,1.237) / 0.067 / 50.10% / 0.157 / 0 / 1 / - / -
Random / 1.112(0.951,1.300) / 0.183 / 50.10% / 0.157 / 0 / 1 / - / -
rs5219
Dominant model / 4 / Fixed / 1.196(1.074,1.334) / 0.001 / 62.10% / 0.048 / 1.02 / 0.308 / 5.51 / 0.031
Random / 1.293(1.057,1.581) / 0.012 / 62.10% / 0.048 / 1.02 / 0.308 / 5.51 / 0.031
Recessive model / 4 / Fixed / 1.293(1.124,1.486) / 0.000 / 0.00% / 0.648 / 1.02 / 0.308 / 1.34 / 0.313
Random / 1.293(1.124,1.486) / 0.000 / 0.00% / 0.648 / 1.02 / 0.308 / 1.34 / 0.313

- only two studies were included and Egger’s test did not provide t value and P value

aZ test was used to determine the significance of the pooled OR

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