Appendix 2 Analysis of distribution of homozygote and allele frequencies for six SSR loci in the Douglas-fir population “OR Klamath Mountains Low” (OrKlMoLo) using MICRO-CHECKER(van Oosterhout et al. 2004). Similar analysis was done for all 18 populations (results are available upon request).(Summary statistics of null allele estimates across all SSR loci is presented for all 18 populations at the end)
Locus: 1C3
Adjusted allele frequencies of amplified alleles based on the four methods of null allele estimation.
ClassObs. allele freq.Oosterhout1Chakraborty2Brookfield13Brookfield23
1500.01160.01170.00710.00850.0038
1580.03490.02350.02140.02540.0113
1600.01160.01170.00710.00850.0038
1660.02330.01170.01420.01690.0075
1680.03490.02350.02140.02540.0113
1700.04650.04760.02850.03390.015
1740.04650.03550.02850.03390.015
1760.02330.02350.01420.01690.0075
1780.06980.03550.04270.05080.0225
1800.05810.04760.03560.04230.0188
1840.01160.01170.00710.00850.0038
1880.03490.02350.02140.02540.0113
1900.08140.05990.04980.05920.0263
1920.02330.02350.01420.01690.0075
1940.0930.05990.0570.06770.03
1960.05810.03550.03560.04230.0188
1980.05810.04760.03560.04230.0188
2000.04650.03550.02850.03390.015
2020.06980.03550.04270.05080.0225
2060.01160.01170.00710.00850.0038
2080.03490.02350.02140.02540.0113
2120.02330.01170.01420.01690.0075
2160.02330.01170.01420.01690.0075
2200.02330.02350.01420.01690.0075
2220.01160.01170.00710.00850.0038
2260.02330.01170.01420.01690.0075
2360.01160.01170.00710.00850.0038
Adjusted genotypesordered by allele size.
Note: Row numbers do not correspond to the original sample numbers.
# / Oosterhout1 / Chakraborty2 / Brookfield13 / Brookfield231 / 150 / 170 / 150 / 170 / 150 / 170 / 150 / 170
2 / 158 / 158 / 158 / 0 / 158 / 158 / 158 / 0
3 / 158 / 198 / 158 / 198 / 158 / 198 / 158 / 198
4 / 160 / 176 / 160 / 176 / 160 / 176 / 160 / 176
5 / 166 / 166 / 166 / 166 / 166 / 166 / 166 / 0
6 / 168 / 168 / 168 / 0 / 168 / 168 / 168 / 0
7 / 168 / 184 / 168 / 184 / 168 / 184 / 168 / 184
8 / 170 / 192 / 170 / 192 / 170 / 192 / 170 / 192
9 / 170 / 200 / 170 / 200 / 170 / 200 / 170 / 200
10 / 170 / 236 / 170 / 236 / 170 / 236 / 170 / 236
11 / 174 / 0 / 174 / 0 / 174 / 0 / 174 / 0
12 / 174 / 190 / 174 / 190 / 174 / 190 / 174 / 190
13 / 174 / 194 / 174 / 194 / 174 / 194 / 174 / 194
14 / 176 / 206 / 176 / 206 / 176 / 206 / 176 / 206
15 / 178 / 0 / 178 / 0 / 178 / 0 / 178 / 0
16 / 178 / 0 / 178 / 0 / 178 / 0 / 178 / 0
17 / 178 / 178 / 178 / 178 / 178 / 178 / 178 / 0
18 / 180 / 0 / 180 / 0 / 180 / 0 / 180 / 0
19 / 180 / 198 / 180 / 198 / 180 / 198 / 180 / 198
20 / 180 / 220 / 180 / 220 / 180 / 220 / 180 / 220
21 / 180 / 220 / 180 / 220 / 180 / 220 / 180 / 220
22 / 188 / 188 / 188 / 0 / 188 / 188 / 188 / 0
23 / 188 / 222 / 188 / 222 / 188 / 222 / 188 / 222
24 / 190 / 0 / 190 / 0 / 190 / 0 / 190 / 0
25 / 190 / 0 / 190 / 0 / 190 / 0 / 190 / 0
26 / 190 / 196 / 190 / 196 / 190 / 196 / 190 / 196
27 / 190 / 198 / 190 / 198 / 190 / 198 / 190 / 198
28 / 192 / 200 / 192 / 200 / 192 / 200 / 192 / 200
29 / 194 / 0 / 194 / 0 / 194 / 0 / 194 / 0
30 / 194 / 0 / 194 / 0 / 194 / 0 / 194 / 0
31 / 194 / 194 / 194 / 0 / 194 / 194 / 194 / 0
32 / 194 / 208 / 194 / 208 / 194 / 208 / 194 / 208
33 / 196 / 0 / 196 / 0 / 196 / 0 / 196 / 0
34 / 196 / 196 / 196 / 0 / 196 / 196 / 196 / 0
35 / 198 / 0 / 198 / 0 / 198 / 0 / 198 / 0
36 / 200 / 0 / 200 / 0 / 200 / 0 / 200 / 0
37 / 202 / 0 / 202 / 0 / 202 / 0 / 202 / 0
38 / 202 / 0 / 202 / 0 / 202 / 0 / 202 / 0
39 / 202 / 202 / 202 / 202 / 202 / 202 / 202 / 0
40 / 208 / 208 / 208 / 0 / 208 / 208 / 208 / 0
41 / 212 / 212 / 212 / 212 / 212 / 212 / 212 / 0
42 / 216 / 216 / 216 / 216 / 216 / 216 / 216 / 0
43 / 226 / 226 / 226 / 226 / 226 / 226 / 226 / 0
Locus: 2G12
Adjusted allele frequencies of amplified alleles based on the four methods of null allele estimation.
ClassObs. allele freq.OosterhoutChakrabortyBrookfield 1Brookfield 2
2400.01410.01420.01260.01280.0124
2420.01410.01420.01260.01280.0124
2440.02110.02140.01880.01910.0186
2460.0070.00710.00630.00640.0062
2480.02820.02860.02510.02550.0247
2500.0070.00710.00630.00640.0062
2520.04230.03590.03770.03830.0371
2540.04230.02860.03770.03830.0371
2560.07750.06550.0690.07010.0681
2580.09150.08070.08160.08290.0804
2600.11970.11190.10670.10840.1052
2620.10560.09620.09410.09560.0928
2640.07750.07310.0690.07010.0681
2660.07040.0580.06280.06380.0619
2680.04230.03590.03770.03830.0371
2700.05630.05060.05020.0510.0495
2720.09150.08840.08160.08290.0804
2740.0070.00710.00630.00640.0062
2760.01410.01420.01260.01280.0124
2780.02110.02140.01880.01910.0186
2820.02110.02140.01880.01910.0186
2840.02110.01420.01880.01910.0186
2880.0070.00710.00630.00640.0062
Adjusted genotypes ordered by allele size.
Note: Row numbers do not correspond to the original sample numbers.
# / Oosterhout / Chakraborty / Brookfield1 / Brookfield21 / 240 / 262 / 240 / 262 / 240 / 262 / 240 / 262
2 / 240 / 264 / 240 / 264 / 240 / 264 / 240 / 264
3 / 242 / 248 / 242 / 248 / 242 / 248 / 242 / 248
4 / 242 / 258 / 242 / 258 / 242 / 258 / 242 / 258
5 / 244 / 266 / 244 / 266 / 244 / 266 / 244 / 266
6 / 244 / 266 / 244 / 266 / 244 / 266 / 244 / 266
7 / 244 / 272 / 244 / 272 / 244 / 272 / 244 / 272
8 / 246 / 268 / 246 / 268 / 246 / 268 / 246 / 268
9 / 248 / 260 / 248 / 260 / 248 / 260 / 248 / 260
10 / 248 / 260 / 248 / 260 / 248 / 260 / 248 / 260
11 / 248 / 276 / 248 / 276 / 248 / 276 / 248 / 276
12 / 250 / 262 / 250 / 262 / 250 / 262 / 250 / 262
13 / 252 / 252 / 252 / 252 / 252 / 252 / 252 / 252
14 / 252 / 262 / 252 / 262 / 252 / 262 / 252 / 262
15 / 252 / 262 / 252 / 262 / 252 / 262 / 252 / 262
16 / 252 / 264 / 252 / 264 / 252 / 264 / 252 / 264
17 / 252 / 268 / 252 / 268 / 252 / 268 / 252 / 268
18 / 254 / 254 / 254 / 254 / 254 / 254 / 254 / 254
19 / 254 / 254 / 254 / 254 / 254 / 254 / 254 / 254
20 / 254 / 270 / 254 / 270 / 254 / 270 / 254 / 270
21 / 254 / 272 / 254 / 272 / 254 / 272 / 254 / 272
22 / 256 / 0 / 256 / 0 / 256 / 0 / 256 / 0
23 / 256 / 256 / 256 / 256 / 256 / 256 / 256 / 256
24 / 256 / 260 / 256 / 260 / 256 / 260 / 256 / 260
25 / 256 / 262 / 256 / 262 / 256 / 262 / 256 / 262
26 / 256 / 264 / 256 / 264 / 256 / 264 / 256 / 264
27 / 256 / 270 / 256 / 270 / 256 / 270 / 256 / 270
28 / 256 / 272 / 256 / 272 / 256 / 272 / 256 / 272
29 / 256 / 272 / 256 / 272 / 256 / 272 / 256 / 272
30 / 256 / 282 / 256 / 282 / 256 / 282 / 256 / 282
31 / 258 / 0 / 258 / 0 / 258 / 0 / 258 / 0
32 / 258 / 258 / 258 / 258 / 258 / 258 / 258 / 0
33 / 258 / 260 / 258 / 260 / 258 / 260 / 258 / 260
34 / 258 / 266 / 258 / 266 / 258 / 266 / 258 / 266
35 / 258 / 266 / 258 / 266 / 258 / 266 / 258 / 266
36 / 258 / 266 / 258 / 266 / 258 / 266 / 258 / 266
37 / 258 / 270 / 258 / 270 / 258 / 270 / 258 / 270
38 / 258 / 272 / 258 / 272 / 258 / 272 / 258 / 272
39 / 258 / 278 / 258 / 278 / 258 / 278 / 258 / 278
40 / 258 / 282 / 258 / 282 / 258 / 282 / 258 / 282
41 / 260 / 0 / 260 / 0 / 260 / 0 / 260 / 0
42 / 260 / 0 / 260 / 0 / 260 / 0 / 260 / 0
43 / 260 / 264 / 260 / 264 / 260 / 264 / 260 / 264
44 / 260 / 264 / 260 / 264 / 260 / 264 / 260 / 264
45 / 260 / 264 / 260 / 264 / 260 / 264 / 260 / 264
46 / 260 / 268 / 260 / 268 / 260 / 268 / 260 / 268
47 / 260 / 272 / 260 / 272 / 260 / 272 / 260 / 272
48 / 260 / 272 / 260 / 272 / 260 / 272 / 260 / 272
49 / 260 / 272 / 260 / 272 / 260 / 272 / 260 / 272
50 / 260 / 274 / 260 / 274 / 260 / 274 / 260 / 274
51 / 260 / 276 / 260 / 276 / 260 / 276 / 260 / 276
52 / 262 / 0 / 262 / 0 / 262 / 0 / 262 / 0
53 / 262 / 262 / 262 / 0 / 262 / 262 / 262 / 0
54 / 262 / 266 / 262 / 266 / 262 / 266 / 262 / 266
55 / 262 / 270 / 262 / 270 / 262 / 270 / 262 / 270
56 / 262 / 272 / 262 / 272 / 262 / 272 / 262 / 272
57 / 262 / 278 / 262 / 278 / 262 / 278 / 262 / 278
58 / 262 / 284 / 262 / 284 / 262 / 284 / 262 / 284
59 / 262 / 288 / 262 / 288 / 262 / 288 / 262 / 288
60 / 264 / 0 / 264 / 0 / 264 / 0 / 264 / 0
61 / 264 / 270 / 264 / 270 / 264 / 270 / 264 / 270
62 / 264 / 272 / 264 / 272 / 264 / 272 / 264 / 272
63 / 264 / 278 / 264 / 278 / 264 / 278 / 264 / 278
64 / 266 / 266 / 266 / 0 / 266 / 266 / 266 / 0
65 / 266 / 266 / 266 / 266 / 266 / 266 / 266 / 266
66 / 268 / 268 / 268 / 268 / 268 / 268 / 268 / 268
67 / 268 / 272 / 268 / 272 / 268 / 272 / 268 / 272
68 / 270 / 270 / 270 / 270 / 270 / 270 / 270 / 270
69 / 270 / 282 / 270 / 282 / 270 / 282 / 270 / 282
70 / 272 / 0 / 272 / 0 / 272 / 0 / 272 / 0
71 / 284 / 284 / 284 / 284 / 284 / 284 / 284 / 0
Locus: 3B2
Adjusted allele frequencies of amplified alleles based on the four methods of null allele estimation.
ClassObs. allele freq.OosterhoutChakrabortyBrookfield 1Brookfield 2
880.04620.04730.04040.04120.0345
980.07690.07180.06730.06860.0575
1060.02310.01550.02020.02060.0172
1080.02310.01550.02020.02060.0172
1100.10.08010.08750.08920.0747
1120.10.08850.08750.08920.0747
1140.00770.00770.00670.00690.0057
1180.00770.00770.00670.00690.0057
1200.07690.06360.06730.06860.0575
1220.06150.05540.05390.05490.046
1240.03850.03920.03370.03430.0287
1260.01540.01550.01350.01370.0115
1280.01540.01550.01350.01370.0115
1320.03080.02330.02690.02740.023
1340.03080.03130.02690.02740.023
1360.01540.01550.01350.01370.0115
1380.02310.02330.02020.02060.0172
1400.00770.00770.00670.00690.0057
1420.00770.00770.00670.00690.0057
1440.03850.03130.03370.03430.0287
1460.03080.02330.02690.02740.023
1480.02310.02330.02020.02060.0172
1500.02310.02330.02020.02060.0172
1520.02310.02330.02020.02060.0172
1540.01540.01550.01350.01370.0115
1560.02310.01550.02020.02060.0172
1600.01540.01550.01350.01370.0115
1620.02310.02330.02020.02060.0172
1640.00770.00770.00670.00690.0057
1660.03080.02330.02690.02740.023
1700.00770.00770.00670.00690.0057
1720.00770.00770.00670.00690.0057
1740.02310.01550.02020.02060.0172
Adjusted genotypes ordered by allele size.
Note: Row numbers do not correspond to the original sample numbers.
# / Oosterhout / Chakraborty / Brookfield1 / Brookfield21 / 88 / 110 / 88 / 110 / 88 / 110 / 88 / 110
2 / 88 / 110 / 88 / 110 / 88 / 110 / 88 / 110
3 / 88 / 112 / 88 / 112 / 88 / 112 / 88 / 112
4 / 88 / 118 / 88 / 118 / 88 / 118 / 88 / 118
5 / 88 / 136 / 88 / 136 / 88 / 136 / 88 / 136
6 / 88 / 156 / 88 / 156 / 88 / 156 / 88 / 156
7 / 98 / 0 / 98 / 0 / 98 / 0 / 98 / 0
8 / 98 / 108 / 98 / 108 / 98 / 108 / 98 / 108
9 / 98 / 120 / 98 / 120 / 98 / 120 / 98 / 120
10 / 98 / 122 / 98 / 122 / 98 / 122 / 98 / 122
11 / 98 / 126 / 98 / 126 / 98 / 126 / 98 / 126
12 / 98 / 132 / 98 / 132 / 98 / 132 / 98 / 132
13 / 98 / 132 / 98 / 132 / 98 / 132 / 98 / 132
14 / 98 / 134 / 98 / 134 / 98 / 134 / 98 / 134
15 / 98 / 134 / 98 / 134 / 98 / 134 / 98 / 134
16 / 106 / 106 / 106 / 106 / 106 / 106 / 106 / 106
17 / 106 / 128 / 106 / 128 / 106 / 128 / 106 / 128
18 / 108 / 108 / 108 / 108 / 108 / 108 / 108 / 108
19 / 110 / 0 / 110 / 0 / 110 / 0 / 110 / 0
20 / 110 / 110 / 110 / 0 / 110 / 110 / 110 / 0
21 / 110 / 110 / 110 / 110 / 110 / 110 / 110 / 0
22 / 110 / 120 / 110 / 120 / 110 / 120 / 110 / 120
23 / 110 / 122 / 110 / 122 / 110 / 122 / 110 / 122
24 / 110 / 124 / 110 / 124 / 110 / 124 / 110 / 124
25 / 110 / 136 / 110 / 136 / 110 / 136 / 110 / 136
26 / 110 / 166 / 110 / 166 / 110 / 166 / 110 / 166
27 / 112 / 0 / 112 / 0 / 112 / 0 / 112 / 0
28 / 112 / 112 / 112 / 0 / 112 / 112 / 112 / 0
29 / 112 / 124 / 112 / 124 / 112 / 124 / 112 / 124
30 / 112 / 124 / 112 / 124 / 112 / 124 / 112 / 124
31 / 112 / 138 / 112 / 138 / 112 / 138 / 112 / 138
32 / 112 / 144 / 112 / 144 / 112 / 144 / 112 / 144
33 / 112 / 150 / 112 / 150 / 112 / 150 / 112 / 150
34 / 112 / 162 / 112 / 162 / 112 / 162 / 112 / 162
35 / 112 / 166 / 112 / 166 / 112 / 166 / 112 / 166
36 / 112 / 170 / 112 / 170 / 112 / 170 / 112 / 170
37 / 114 / 146 / 114 / 146 / 114 / 146 / 114 / 146
38 / 120 / 0 / 120 / 0 / 120 / 0 / 120 / 0
39 / 120 / 120 / 120 / 120 / 120 / 120 / 120 / 0
40 / 120 / 152 / 120 / 152 / 120 / 152 / 120 / 152
41 / 120 / 152 / 120 / 152 / 120 / 152 / 120 / 152
42 / 120 / 154 / 120 / 154 / 120 / 154 / 120 / 154
43 / 120 / 172 / 120 / 172 / 120 / 172 / 120 / 172
44 / 122 / 122 / 122 / 122 / 122 / 122 / 122 / 0
45 / 122 / 148 / 122 / 148 / 122 / 148 / 122 / 148
46 / 122 / 148 / 122 / 148 / 122 / 148 / 122 / 148
47 / 122 / 160 / 122 / 160 / 122 / 160 / 122 / 160
48 / 122 / 160 / 122 / 160 / 122 / 160 / 122 / 160
49 / 124 / 140 / 124 / 140 / 124 / 140 / 124 / 140
50 / 124 / 162 / 124 / 162 / 124 / 162 / 124 / 162
51 / 126 / 162 / 126 / 162 / 126 / 162 / 126 / 162
52 / 128 / 144 / 128 / 144 / 128 / 144 / 128 / 144
53 / 132 / 132 / 132 / 132 / 132 / 132 / 132 / 0
54 / 134 / 150 / 134 / 150 / 134 / 150 / 134 / 150
55 / 134 / 152 / 134 / 152 / 134 / 152 / 134 / 152
56 / 138 / 148 / 138 / 148 / 138 / 148 / 138 / 148
57 / 138 / 154 / 138 / 154 / 138 / 154 / 138 / 154
58 / 142 / 150 / 142 / 150 / 142 / 150 / 142 / 150
59 / 144 / 144 / 144 / 144 / 144 / 144 / 144 / 0
60 / 144 / 174 / 144 / 174 / 144 / 174 / 144 / 174
61 / 146 / 146 / 146 / 146 / 146 / 146 / 146 / 0
62 / 146 / 164 / 146 / 164 / 146 / 164 / 146 / 164
63 / 156 / 156 / 156 / 156 / 156 / 156 / 156 / 156
64 / 166 / 166 / 166 / 166 / 166 / 166 / 166 / 0
65 / 174 / 174 / 174 / 174 / 174 / 174 / 174 / 174
Locus: 3F1
Adjusted allele frequencies of amplified alleles based on the four methods of null allele estimation.
ClassObs. allele freq.OosterhoutChakrabortyBrookfield 1Brookfield 2
1520.0250.02530.01950.02060.0085
1600.06250.05130.04870.05160.0211
1620.01250.01260.00970.01030.0042
1640.06250.05130.04870.05160.0211
1700.0250.02530.01950.02060.0085
1720.03750.03820.02920.03090.0127
1740.01250.01260.00970.01030.0042
1760.0250.01260.01950.02060.0085
1800.01250.01260.00970.01030.0042
1860.0250.01260.01950.02060.0085
1880.01250.01260.00970.01030.0042
1920.0250.01260.01950.02060.0085
1960.01250.01260.00970.01030.0042
2000.0250.01260.01950.02060.0085
2020.01250.01260.00970.01030.0042
2040.10.0780.0780.08250.0338
2060.050.03820.0390.04130.0169
2080.0750.06460.05850.06190.0254
2100.08750.0780.06820.07220.0296
2120.050.03820.0390.04130.0169
2140.06250.05130.04870.05160.0211
2160.11250.0780.08770.09280.038
2180.01250.01260.00970.01030.0042
2200.03750.03820.02920.03090.0127
2220.0250.02530.01950.02060.0085
Adjusted genotypes ordered by allele size.
Note: Row numbers do not correspond to the original sample numbers.
# / Oosterhout / Chakraborty / Brookfield1 / Brookfield21 / 152 / 204 / 152 / 204 / 152 / 204 / 152 / 204
2 / 152 / 204 / 152 / 204 / 152 / 204 / 152 / 204
3 / 160 / 160 / 160 / 0 / 160 / 160 / 160 / 0
4 / 160 / 180 / 160 / 180 / 160 / 180 / 160 / 180
5 / 160 / 204 / 160 / 204 / 160 / 204 / 160 / 204
6 / 160 / 220 / 160 / 220 / 160 / 220 / 160 / 220
7 / 162 / 208 / 162 / 208 / 162 / 208 / 162 / 208
8 / 164 / 164 / 164 / 0 / 164 / 164 / 164 / 0
9 / 164 / 206 / 164 / 206 / 164 / 206 / 164 / 206
10 / 164 / 210 / 164 / 210 / 164 / 210 / 164 / 210
11 / 164 / 218 / 164 / 218 / 164 / 218 / 164 / 218
12 / 170 / 212 / 170 / 212 / 170 / 212 / 170 / 212
13 / 170 / 220 / 170 / 220 / 170 / 220 / 170 / 220
14 / 172 / 204 / 172 / 204 / 172 / 204 / 172 / 204
15 / 172 / 212 / 172 / 212 / 172 / 212 / 172 / 212
16 / 172 / 220 / 172 / 220 / 172 / 220 / 172 / 220
17 / 174 / 210 / 174 / 210 / 174 / 210 / 174 / 210
18 / 176 / 176 / 176 / 176 / 176 / 176 / 176 / 0
19 / 186 / 186 / 186 / 186 / 186 / 186 / 186 / 0
20 / 188 / 206 / 188 / 206 / 188 / 206 / 188 / 206
21 / 192 / 192 / 192 / 192 / 192 / 192 / 192 / 0
22 / 196 / 214 / 196 / 214 / 196 / 214 / 196 / 214
23 / 200 / 200 / 200 / 200 / 200 / 200 / 200 / 0
24 / 202 / 216 / 202 / 216 / 202 / 216 / 202 / 216
25 / 204 / 0 / 204 / 0 / 204 / 0 / 204 / 0
26 / 204 / 204 / 204 / 0 / 204 / 204 / 204 / 0
27 / 206 / 206 / 206 / 206 / 206 / 206 / 206 / 0
28 / 208 / 0 / 208 / 0 / 208 / 0 / 208 / 0
29 / 208 / 210 / 208 / 210 / 208 / 210 / 208 / 210
30 / 208 / 210 / 208 / 210 / 208 / 210 / 208 / 210
31 / 208 / 222 / 208 / 222 / 208 / 222 / 208 / 222
32 / 210 / 0 / 210 / 0 / 210 / 0 / 210 / 0
33 / 210 / 216 / 210 / 216 / 210 / 216 / 210 / 216
34 / 212 / 212 / 212 / 212 / 212 / 212 / 212 / 0
35 / 214 / 214 / 214 / 0 / 214 / 214 / 214 / 0
36 / 214 / 216 / 214 / 216 / 214 / 216 / 214 / 216
37 / 214 / 222 / 214 / 222 / 214 / 222 / 214 / 222
38 / 216 / 0 / 216 / 0 / 216 / 0 / 216 / 0
39 / 216 / 0 / 216 / 0 / 216 / 0 / 216 / 0
40 / 216 / 216 / 216 / 216 / 216 / 216 / 216 / 0
Locus: 3G9
Adjusted allele frequencies of amplified alleles based on the four methods of null allele estimation.
ClassObs. allele freq.OosterhoutChakrabortyBrookfield 1Brookfield 2
1360.02270.01530.01950.02010.017
1380.12880.11240.11070.11410.0966
1400.23480.21180.20190.20810.1761
1420.21970.16520.18890.19470.1647
1440.06060.05450.05210.05370.0454
1460.0530.03860.04560.0470.0398
1480.02270.0230.01950.02010.017
1500.01520.01530.0130.01340.0114
1540.04550.04650.03910.04030.0341
1560.0530.05450.04560.0470.0398
1580.03030.03080.02610.02680.0227
1600.02270.0230.01950.02010.017
1620.03790.03080.03260.03360.0284
1640.00760.00760.00650.00670.0057
1680.00760.00760.00650.00670.0057
1700.00760.00760.00650.00670.0057
1720.00760.00760.00650.00670.0057
1760.01520.01530.0130.01340.0114
1860.00760.00760.00650.00670.0057
Adjusted genotypes ordered by allele size.
Note: Row numbers do not correspond to the original sample numbers.
# / Oosterhout / Chakraborty / Brookfield1 / Brookfield21 / 136 / 136 / 136 / 136 / 136 / 136 / 136 / 136
2 / 136 / 170 / 136 / 170 / 136 / 170 / 136 / 170
3 / 138 / 0 / 138 / 0 / 138 / 0 / 138 / 0
4 / 138 / 0 / 138 / 0 / 138 / 0 / 138 / 0
5 / 138 / 138 / 138 / 138 / 138 / 138 / 138 / 0
6 / 138 / 140 / 138 / 140 / 138 / 140 / 138 / 140
7 / 138 / 140 / 138 / 140 / 138 / 140 / 138 / 140
8 / 138 / 142 / 138 / 142 / 138 / 142 / 138 / 142
9 / 138 / 144 / 138 / 144 / 138 / 144 / 138 / 144
10 / 138 / 150 / 138 / 150 / 138 / 150 / 138 / 150
11 / 138 / 156 / 138 / 156 / 138 / 156 / 138 / 156
12 / 138 / 156 / 138 / 156 / 138 / 156 / 138 / 156
13 / 138 / 158 / 138 / 158 / 138 / 158 / 138 / 158
14 / 138 / 158 / 138 / 158 / 138 / 158 / 138 / 158
15 / 138 / 160 / 138 / 160 / 138 / 160 / 138 / 160
16 / 138 / 176 / 138 / 176 / 138 / 176 / 138 / 176
17 / 140 / 0 / 140 / 0 / 140 / 0 / 140 / 0
18 / 140 / 0 / 140 / 0 / 140 / 0 / 140 / 0
19 / 140 / 0 / 140 / 0 / 140 / 0 / 140 / 0
20 / 140 / 0 / 140 / 0 / 140 / 0 / 140 / 0
21 / 140 / 140 / 140 / 140 / 140 / 140 / 140 / 0
22 / 140 / 140 / 140 / 140 / 140 / 140 / 140 / 0
23 / 140 / 142 / 140 / 142 / 140 / 142 / 140 / 142
24 / 140 / 142 / 140 / 142 / 140 / 142 / 140 / 142
25 / 140 / 142 / 140 / 142 / 140 / 142 / 140 / 142
26 / 140 / 142 / 140 / 142 / 140 / 142 / 140 / 142
27 / 140 / 144 / 140 / 144 / 140 / 144 / 140 / 144
28 / 140 / 144 / 140 / 144 / 140 / 144 / 140 / 144
29 / 140 / 146 / 140 / 146 / 140 / 146 / 140 / 146
30 / 140 / 154 / 140 / 154 / 140 / 154 / 140 / 154
31 / 140 / 154 / 140 / 154 / 140 / 154 / 140 / 154
32 / 140 / 156 / 140 / 156 / 140 / 156 / 140 / 156
33 / 140 / 156 / 140 / 156 / 140 / 156 / 140 / 156
34 / 140 / 158 / 140 / 158 / 140 / 158 / 140 / 158
35 / 140 / 160 / 140 / 160 / 140 / 160 / 140 / 160
36 / 140 / 162 / 140 / 162 / 140 / 162 / 140 / 162
37 / 140 / 162 / 140 / 162 / 140 / 162 / 140 / 162
38 / 140 / 164 / 140 / 164 / 140 / 164 / 140 / 164
39 / 140 / 168 / 140 / 168 / 140 / 168 / 140 / 168
40 / 142 / 0 / 142 / 0 / 142 / 0 / 142 / 0
41 / 142 / 0 / 142 / 0 / 142 / 0 / 142 / 0
42 / 142 / 0 / 142 / 0 / 142 / 0 / 142 / 0
43 / 142 / 0 / 142 / 0 / 142 / 142 / 142 / 0
44 / 142 / 142 / 142 / 142 / 142 / 142 / 142 / 0
45 / 142 / 142 / 142 / 142 / 142 / 142 / 142 / 0
46 / 142 / 142 / 142 / 142 / 142 / 142 / 142 / 0
47 / 142 / 142 / 142 / 142 / 142 / 142 / 142 / 142
48 / 142 / 142 / 142 / 142 / 142 / 142 / 142 / 142
49 / 142 / 146 / 142 / 146 / 142 / 146 / 142 / 146
50 / 142 / 148 / 142 / 148 / 142 / 148 / 142 / 148
51 / 142 / 154 / 142 / 154 / 142 / 154 / 142 / 154
52 / 142 / 154 / 142 / 154 / 142 / 154 / 142 / 154
53 / 142 / 156 / 142 / 156 / 142 / 156 / 142 / 156
54 / 142 / 160 / 142 / 160 / 142 / 160 / 142 / 160
55 / 144 / 0 / 144 / 0 / 144 / 144 / 144 / 0
56 / 144 / 154 / 144 / 154 / 144 / 154 / 144 / 154
57 / 144 / 156 / 144 / 156 / 144 / 156 / 144 / 156
58 / 144 / 176 / 144 / 176 / 144 / 176 / 144 / 176
59 / 146 / 146 / 146 / 146 / 146 / 146 / 146 / 0
60 / 146 / 146 / 146 / 146 / 146 / 146 / 146 / 0
61 / 146 / 156 / 146 / 156 / 146 / 156 / 146 / 156
62 / 148 / 158 / 148 / 158 / 148 / 158 / 148 / 158
63 / 148 / 172 / 148 / 172 / 148 / 172 / 148 / 172
64 / 150 / 154 / 150 / 154 / 150 / 154 / 150 / 154
65 / 162 / 162 / 162 / 162 / 162 / 162 / 162 / 0
66 / 162 / 186 / 162 / 186 / 162 / 186 / 162 / 186
Locus: 4A7
There is no significant evidence for the presence of a null allele at this locus.
The estimated allele frequencies have therefore not been calculated.
Summary statistics of null allele estimates across all loci
OrKlMoLo (1)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.2779 / 0.3878 / 0.2721 / 0.6774
2G12 / yes / 0.0976 / 0.1089 / 0.0946 / 0.1215
3B2 / yes / 0.1112 / 0.1248 / 0.1081 / 0.2531
3F1 / yes / 0.1801 / 0.2203 / 0.1749 / 0.662
3G9 / yes / 0.1251 / 0.1402 / 0.114 / 0.2501
4A7 / yes / 0.0284 / 0.0298 / 0.0283 / 0.0568
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
OrKlMoHi (2)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3174 / 0.4706 / 0.3129 / 0.6699
2G12 / yes / 0.1141 / 0.1271 / 0.1082 / 0.1649
3B2 / yes / 0.0909 / 0.0999 / 0.0887 / 0.3925
3F1 / yes / 0.1896 / 0.2343 / 0.1855 / 0.6624
3G9 / yes / 0.1313 / 0.1531 / 0.1244 / 0.2442
4A7 / no / 0.0103 / 0.011 / 0.0106 / 0.0345
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
OrCoSoLo (3)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3125 / 0.4581 / 0.3041 / 0.6343
2G12 / yes / 0.1377 / 0.1591 / 0.1318 / 0.2305
3B2 / yes / 0.0942 / 0.103 / 0.0912 / 0.2698
3F1 / yes / 0.3445 / 0.5325 / 0.3376 / 0.7093
3G9 / yes / 0.226 / 0.2939 / 0.2107 / 0.3316
4A7 / yes / 0.0263 / 0.0282 / 0.0267 / 0.1269
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
OrCoSoHi (4)
There are insufficient samples remaining for analysis to proceed.
OrCoNoLo (5)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3266 / 0.4903 / 0.3212 / 0.5711
2G12 / yes / 0.0911 / 0.0957 / 0.0822 / 0.338
3B2 / yes / 0.117 / 0.1334 / 0.1152 / 0.3636
3F1 / yes / 0.2601 / 0.3535 / 0.2526 / 0.6613
3G9 / yes / 0.1684 / 0.202 / 0.156 / 0.2832
4A7 / no / 0.0197 / 0.0206 / 0.0197 / 0.0657
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
OrCoNoHi (6)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.1786 / 0.2168 / 0.1694 / 0.4301
2G12 / yes / 0.0947 / 0.1011 / 0.0857 / 0.2012
3B2 / no / 0.0018 / 0 / 0 / 0.1961
3F1 / yes / 0.2595 / 0.3642 / 0.2381 / 0.7231
3G9 / no / 0.0721 / 0.082 / 0.0693 / 0.1911
4A7 / no / -0.031 / -0.0297 / -0.0297 / 0
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3F1, showing signs of a null allele.
OrCoEaLo (7)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.2735 / 0.3826 / 0.2655 / 0.7062
2G12 / yes / 0.0798 / 0.0862 / 0.0763 / 0.2308
3B2 / yes / 0.0833 / 0.0903 / 0.0806 / 0.3506
3F1 / yes / 0.164 / 0.1951 / 0.158 / 0.587
3G9 / yes / 0.1999 / 0.2443 / 0.1773 / 0.1773
4A7 / yes / 0.0905 / 0.1014 / 0.0887 / 0.1484
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
WaCoSoLo (8)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.1769 / 0.2232 / 0.1747 / 0.4689
2G12 / no / 0.0341 / 0.0365 / 0.0338 / 0.199
3B2 / no / 0.0324 / 0.0328 / 0.0308 / 0.415
3F1 / yes / 0.2032 / 0.2572 / 0.197 / 0.5877
3G9 / yes / 0.1941 / 0.2413 / 0.1814 / 0.3092
4A7 / no / 0.0133 / 0.014 / 0.0133 / 0.1484
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 3F1, 3G9, showing signs of a null allele.
WaCoNoLo (9)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3809 / 0.6522 / 0.3488 / 0.8359
2G12 / no / 0.0547 / 0.0523 / 0.0463 / 0.0463
3B2 / no / 0.008 / 0.009 / 0.0086 / 0.2999
3F1 / yes / 0.1424 / 0.163 / 0.1316 / 0.4185
3G9 / no / 0.0987 / 0.0986 / 0.0805 / 0.3053
4A7 / yes / 0.1132 / 0.1269 / 0.1062 / 0.2239
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 3F1, 4A7, showing signs of a null allele.
OrCaSoLo (10)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3678 / 0.5895 / 0.3603 / 0.7113
2G12 / yes / 0.0872 / 0.0944 / 0.0827 / 0.1623
3B2 / yes / 0.0896 / 0.0994 / 0.0884 / 0.1164
3F1 / yes / 0.2556 / 0.3455 / 0.25 / 0.5003
3G9 / yes / 0.1739 / 0.2096 / 0.1634 / 0.2998
4A7 / yes / 0.0302 / 0.0315 / 0.0297 / 0.061
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
OrCaSoHi (11)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.2757 / 0.3847 / 0.2716 / 0.5989
2G12 / yes / 0.075 / 0.081 / 0.0723 / 0.1663
3B2 / yes / 0.0772 / 0.084 / 0.0758 / 0.1746
3F1 / yes / 0.2559 / 0.3463 / 0.2507 / 0.4862
3G9 / yes / 0.1256 / 0.1407 / 0.1158 / 0.2289
4A7 / no / 0.0133 / 0.0141 / 0.0136 / 0.0699
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
OrCaNoLo (12)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3123 / 0.4621 / 0.3061 / 0.6912
2G12 / yes / 0.0746 / 0.0782 / 0.0695 / 0.2537
3B2 / no / 0.0166 / 0.0179 / 0.0172 / 0.245
3F1 / yes / 0.2717 / 0.3754 / 0.2656 / 0.5
3G9 / yes / 0.1743 / 0.2073 / 0.1595 / 0.3377
4A7 / yes / 0.0473 / 0.0497 / 0.0461 / 0.1226
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3F1, 3G9, 4A7, showing signs of a null allele.
OrCaNoHi (13)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3478 / 0.5402 / 0.3409 / 0.6285
2G12 / yes / 0.095 / 0.1047 / 0.0906 / 0.2675
3B2 / yes / 0.1018 / 0.114 / 0.0997 / 0.3015
3F1 / yes / 0.2614 / 0.3568 / 0.2561 / 0.6118
3G9 / yes / 0.1358 / 0.1548 / 0.1238 / 0.2844
4A7 / no / -0.0005 / -0.0001 / -0.0001 / 0.0216
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
OrCaEaHi (14)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3832 / 0.6341 / 0.3741 / 0.6713
2G12 / yes / 0.0862 / 0.0927 / 0.0809 / 0.1788
3B2 / yes / 0.0588 / 0.0625 / 0.0571 / 0.2622
3F1 / yes / 0.2396 / 0.3181 / 0.2318 / 0.6116
3G9 / yes / 0.1399 / 0.1597 / 0.1288 / 0.2251
4A7 / no / 0.0313 / 0.0332 / 0.0312 / 0.0312
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
WaCaSoLo (15)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.2984 / 0.4282 / 0.29 / 0.5967
2G12 / yes / 0.1141 / 0.1247 / 0.1047 / 0.3557
3B2 / yes / 0.0762 / 0.0833 / 0.0748 / 0.3417
3F1 / yes / 0.2611 / 0.3559 / 0.2546 / 0.6057
3G9 / yes / 0.1657 / 0.1886 / 0.1415 / 0.2872
4A7 / yes / 0.091 / 0.0968 / 0.0844 / 0.1763
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
WaCaSoHi (16)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3114 / 0.4588 / 0.3044 / 0.7181
2G12 / yes / 0.158 / 0.1865 / 0.1488 / 0.2822
3B2 / yes / 0.0841 / 0.0917 / 0.0815 / 0.2003
3F1 / yes / 0.1651 / 0.1986 / 0.1612 / 0.4724
3G9 / yes / 0.1449 / 0.1739 / 0.1384 / 0.2754
4A7 / yes / 0.0354 / 0.0379 / 0.0356 / 0.0825
All loci show evidence for a null allele.
This population is probably not in Hardy Weinberg equilibrium.
WaCaNoLo (17)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3094 / 0.4549 / 0.3003 / 0.7239
2G12 / yes / 0.1061 / 0.1211 / 0.0999 / 0.2991
3B2 / yes / 0.1224 / 0.1417 / 0.1201 / 0.1924
3F1 / yes / 0.178 / 0.2163 / 0.1704 / 0.6392
3G9 / yes / 0.1462 / 0.1712 / 0.1371 / 0.3275
4A7 / no / 0.0185 / 0.0191 / 0.0182 / 0.0949
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3B2, 3F1, 3G9, showing signs of a null allele.
WaCaNoHi (18)
Locus / Null Present / Oosterhout / Chakraborty / Brookfield 1 / Brookfield 21C3 / yes / 0.3046 / 0.4437 / 0.2916 / 0.7521
2G12 / yes / 0.1055 / 0.1201 / 0.1012 / 0.3301
3B2 / no / 0.0373 / 0.0383 / 0.0357 / 0.2343
3F1 / yes / 0.2295 / 0.3039 / 0.2254 / 0.5422
3G9 / no / 0.0583 / 0.0656 / 0.0568 / 0.3254
4A7 / yes / 0.0766 / 0.0839 / 0.075 / 0.2444
Several loci show evidence for a null allele.
This population is possibly in Hardy Weinberg equilibrium with loci 1C3, 2G12, 3F1, 4A7, showing signs of a null allele.
1van Oosterhout C, Weetman D, Hutchinson WF (2006) Estimationand adjustment of microsatellite null alleles in nonequilibriumpopulations. Molecular Ecology Notes6: 255–256.
2Chakraborty R, De Andrade M, Daiger SP, Budowle B (1992)Apparent heterozygote deficiencies observed in DNA typingdata and their implications in forensic applications.Annals ofHuman Genetics56: 45–57.
3Brookfield JFY (1996) A simple new method for estimating nullallele frequency from heterozygote deficiency.Molecular Ecology5: 453–455.
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