Supplementary Information

An investigation of in vivo wound healing activity of biologically synthesized silver nanoparticles

Abhishek Kaler1, Amit Kumar Mittal1, Mahesh Katariya2#, Harshad Harde2#, Ashish Kumar Agrawal2#, Sanyog Jain2 and Uttam Chand Banerjee1*

1Department of Pharmaceutical Technology (Biotechnology)

National Institute of Pharmaceutical Education and Research

Sector-67, SAS Nagar-160062, Punjab, INDIA

2Centre for Pharmaceutical Nanotechnology

Department of Pharmaceutics

National Institute of Pharmaceutical Education and Research

Sector-67, SAS Nagar-160062, Punjab, INDIA

*Corresponding author. Tel:+91-172-2214682-687, Extn 2142; fax: +91-172-2214692

E-mail:

# These authors contributed equally to the manuscript

List of Tables

Table S-1.Microorganisms screened for the synthesis of silver nanoparticles

Table S-2.Reaction of microbial cells with silver nitrate (1mM) in deionised water

Table S-3. Stability study of biologically synthesized nanosilver gel

Figure S-1.Characterization of synthesised silver nanoparticles

Figure S-2. Effect of gelation on the characterization of particle size by TEM

Table S-1

Sr. No. / Microorganism / Growth medium
1. / Lactobacillus fermentum MTCC 903 / MRS medium
2. / Lactobacillus plantarum MTCC 1746 / NB medium
3. / Lactobacillus rhamnosus MTCC 1408 / MRS medium
4. / Lactobacillus bulgaricus NCIM 2056 / MRS medium
5. / Lactobacillus bulgaricus NCIM 2057 / MRS medium
6. / Lactobacillus brevis NCIM 2356 / MRS medium
7. / Bacillus coagulans MTCC 492 / NB medium
8. / Burkholderia cenocepacia MTCC 5427 / NB medium
9. / Geotrichum candidum NCIM 980 / YPD medium
10. / Saccharomyces cerevisiae TYPE I (Dried powder purchased from SIGMA) / Lyophilized
11. / Saccharomyces cerevisiae TYPE II (Dried powder purchased from SIGMA) / Lyophilized
12. / Pseudomonas putida MTCC 102 / NB medium
13. / Phanerochaete.chrysosporium MTCC 787 / NB medium
14. / Escherichia coli MTCC 433 / NB medium
15. / Bacillus megaterium MTCC 428 / NB medium
16. / Phaffia rhodozyma / NB medium
17. / Rhodococcus erythropolis MTCC 1526 / NB medium
18. / Acinetobacter baumannii MTCC 1425 / YPD medium
19 / Saccharomyces boulardii(Probiotic) / YPD medium
20 / Pichia anomala MTCC 237 / YPD medium
21 / Rhodotorula bogariensis MTCC 2764 / NA medium
22 / Bacillus stereothermophillus / NA medium

Table S-2

Microorganism / Change in color of cell mass / λmax (nm) of cell supernatant / Absorbance
Lactobacillus fermentum MTCC 903 / No change / No peak / Nil
Lactobacillus plantarum MTCC 1746 / Dark brown / No peak / Nil
Lactobacillus rhamnosus MTCC 1408 / No change / No peak / Nil
Lactobacillus bulgaricus NCIM 2056 / No change / No peak / Nil
Lactobacillus bulgaricus NCIM 2057 / No change / No peak / Nil
Lactobacillus brevis NCIM 2356 / No change / No peak / Nil
Bacillus coagulans MTCC 492 / No change / No peak / Nil
Burkholderia cenocepacia MTCC 5427 / No change / No peak / Nil
Geotrichum candidum NCIM 980 / Light brown / 420 / 1.233
Saccharomyces cerevisiae TYPE I (Dried powder purchased from SIGMA) / No change / 400.6 / 0.945
Saccharomyces cerevisiae TYPE II (Dried powder purchased from SIGMA) / No change / 426.1 / 1.026
Pseudomonas putida MTCC 102 / No change / No peak / Nil
Phanerochaete chrysosporium MTCC 787 / No change / No peak / Nil
Escherichia coli (MTCC 433) / No change / No peak / Nil
Bacillus megaterium MTCC 428 / No change / No peak / Nil
Phaffia rhodozyme / No change / No peak / Nil
Rhodococcus erythropolis MTCC 1526 / No change / No peak / Nil
Acinetobacter baumannii MTCC 1425 / No change / No peak / Nil
Saccharomyces boulardii (Probiotic) / No change / 417 / 0.715
Pichhia anomala MTCC 237 / No change / No peak / Nil
Rhodotorula bogariensis MTCC 2764 / No change / No peak / Nil
Bacillus stereothermophillus / No change / No peak / Nil

1

Table S-3

a) Characteristics of Ag-NPs gel
Storage conditions / 2-8°C / 25°C/60% RH / 40°C/75% RH
Particle size (nm) / PDI / Viscosity (Pa.s) / Spreadability (cm²) / Particle size (nm) / PDI / Viscosity
( Pa.s) / Spreadability (cm²) / Particle size (nm) / PDI / Viscosity
( Pa.s) / Spreadability (cm²)
Initial / 7±4 / 0.32±0.01 / 24.63±0.35 / 11.52±1.52 / 7±4 / 0.32±0.01 / 24.63±0.35 / 11.52±1.52 / 7±4 / 0.32±0.01 / 24.63±0.35 / 11.52±1.52
1st month / 8±3 / 0.33±0.05 / 24.87±0.41 / 11.32±1.3 / 7±3 / 0.32±0.01 / 24.75±0.38 / 11.39±0.4 / 8±4 / 0.36±0.025 / 24.74±0.26 / 11.7±0.8
2nd month / 7±3 / 0.31±0.03 / 25.12±0.66 / 11.29±1.4 / 7±3 / 0.33±0.01 / 24.93±0.43 / 11.18±0.8 / 11±3 / 0.37±0.034 / 24.89±0.56 / 11.9±0.6
3rd month / 9±5 / 0.31±0.03 / 25.42±0.32 / 11.17±1.11 / 7±4 / 0.35±0.02 / 25.36±0.37 / 12.1±1.2 / Aggregation / 25.32±0.61 / 12.3±1.0

1

Figure S-1

Figure S-2


1