Am TCRD : THVQPS VFVLRS DDAAPCLVKDFSLKQ-AEIFM NSSSVSETGIETSAPVLSKSGKYSA VRVREFPVSD

Ig Domain * *

Am TCRD : THVQPS--VFVLRS------DDAAPCLVKDFSLKQ-AEIFM------NSSSVSETGIETSAPVLSKSGKYSA------VRVREFPVSD---HVTCAWGHGGSVVMSNDVMETQKE

Cm TCRD : KPVNPK--VTMFYPAIKS---NP--PASDPAALCLADEYIPKE-LSILV--HD-INDMDLTKSATTSSSIAIIDHKLYHK------TSFLTVPQDT—-TGMRCIVQHEGKNVSAEAKYT----

Cc TCRD : -ETAPV--LSILSP------FKGDGPDICLAAGFFPQE-KNMTL-----TLEGNEPKSLTSSNAPLFSTTKTYYY------AGFSDNKKIN-----ECKMDGQTADKTSDTPETTDKP

Dr TCRA : TEVKPN--IYQVGN------SCLATDFTKHN--EFNI------TDSPFLETSAVRYSGQSYYSS------FKFSSNDCKE---TGVCSSSGGGTDLER------

Dr TCRB : NVTEPE--VKILPPSPKELCSQNTNDKKLSTLVCVATGFYPDH-VSMSW-----KVNDQERKDNISTDHIAIQDKTTLMYNISSRMVYVSEWMDPN—-NKFTCTVRFFNGDHYINVSYTTKGQ

Dr TCRD : TVNSPPAFLSVLSP------IKGHGSDICVAAGFFPQQ-KTMIL-----TSEDGNTVNQETSNAVLSLSSKNYYY------VGSSEKKIQE------CVMDGKTAKADK---IDKPSG

Dr TCRG : SVVTPK--LSGYLNK------ANEKPAALCQAKDMFPDL-VSFKW---E-KKSSSGGWTEVSNDQIVEHSHNEDPV------TSMVILNTPE-DNIYRCTVTHEGSKDPQQIEIKKKDE

Dl TCRG :QIVKPV--VSVYPAASRA---H---LEGKISLLCVASDMFPPL-VRFSW--KRQKDNGPLEKVTSADKKQLKLKESGCTA------TILQIPQRESSTYKYYCSVQHEGETVEAQIKQEVSTL

Gg TCRD :RDSIPE--VIVMKSKKHK---E---DGDTGKAACLARNFYKKN-ISLEM------SSNEVVYEPKAPIVTSNGMYNT------IKVVKVTKQS---EVTCTARLSNGNFTANSTTPEMKA

GcTCRD :SIVKPK--LSAFYPPKSS---S---KDAVQAAVCLASDFFPKD-ISLQL------AFGDKPKANVTRPSVLKPD-GSYVA------FSFLFNPENE—-ANFVCEAVHENQHSHANITLGTKSG

HfTCRD :RPENPK--LSIFYPSLAR---SDDLDPDETAAVCLASEFTPKE-IELSV------VWDINHKSNVTRSSILLNDGYYWS------SGFLPFPKDQKPVNVTCEAKHNGDTIVQQNIKEPTAA

Hs TCRD :PHTKPS--VFVMKN------GTNVACLVKEFYPKD-IRINL------VSSKKITEFDPAIVISPSGKYNA------VKLGKYEDSN---SVTCSVQHDNKTVHSTDFEVKTDS

IpTCRD :PRSLPT--LSILTSH------DSNEKLEICLAAGFFPKE-GEVSL-----YTGDNVTPENHTVENAAMSAAGTYYY------AAFSKDGIKK------CAMKDVSLDKNDVKPTAQIPS

IpTCRG :RVKEPQ--VYVYPVSTPE------KGEKSFLLCHARGMFPDL-VRFTW-----QAKDQGGKNVDLRGDERLEQRDEVPEVRITSMLIVGKDKAKN--NNFICTVKHDSSVKDKELPIPREED

Mm TCRD :PPAKPS--VFIMKN------GTNVACLVKDFYPKE-VTISL------RSSKKIVEFDPAIVISPSGKYSA------VKLGQYGDSN---SVTCSVQHNSETVHSTDFEPYANS

OvTCRD :PAASPS--VFVMKN------GTNVACLVKEFYPKE-VTISL------QSSKKIIEYEPAIVVSPGGRYSA------VKLGQYNDPD---SVTCSVEHNKKTWHSSDFEPKKDI

Po TCRA :DEYEPS--YYELE------DQDTTITACLATGFSKHN-ATLEH------DLFKNGSTNMTGAARASEESLY------SQVALWSDAS-----QCESRTGKNESEVCADVLKK--

Po TCRB :AVKSPI--VKVFRPSSKE-CRNPIDNEREKTLVCVASDFYPDH-VSVYWQIIQLNVTSGVNVTRGVTTDEAALRKDKVYTITSRLKVSAEDWYKPE—-WNFECIVRFFNGTHDTYHKDSISGP

Po TCRD :SPVKPS--LSVLSP------LDPGGPRVCLAAGFRPQE-VAMVL------NGSNSIDISEEEAVASLRHNSFFF------TGFSDGAISS------CEMNDVSSSNDHE------

Po TCRG :------RMYRTASRD---D---LDGKSVLLCVASNMSPPL-VHFSW--KRQKEGGPLEDLTSAQGEQLELRVPERTA------AIMVVDRDASYTYKYRCNVRHEGGPTERVVPALPPST

Re TCRD :DKQEPE--LSIHYPPVHQ---SG--KTDQTATVCLLGPFYPNK-VTVNI----TLDGTTDNGSRTVLTEDVLTDKGYYRT------SGFREFSHDLQPKTIGCSAKHGDKYIETKKEITISAP

SsTCRD :IAARPT--LSILTPLHKS------GDDDQPKVCLATGFFPST-KNMNL------ILGNNDTVQLSTTKAALSSTRTYFF------AGFSKENIKR------CEMDGNVEPPVDDKDDSTDVS

ScTCRB :------KQGKGETIVCVASEFYPDH-VSVSW-----TINGSIVREGVATDNAAL-RVGKYYRITSRLRVAAETWYNPS—-IKFTCIVTFYNVTNTDYPAHIFGET

TrTCRD :TVLSPT--LFILTPLL------SEAPPADVCLAADFRPKE-GEMVV------NGRVQKTSNAVLSLGHKSFFF------AGFNRTIES------CRLHNSSVPVDREQDTDQDTD

TnTCRD :LVLQPT--LFILNPQ------PPAEPVDVCLAAHFSPRQ-GEMGV------NGLKQETSGAVMSHRHGSFFF------AGFNRTVKD------CRLQESSDSVDPAGKDQGTD-

XtTCRD :---APS--VFVLKP------QKGDSPTACLVKDFTPKEDVEIYM------NSTQSNGSIISASPVVSLNGTYSA------VHVDRLGTEG----LHCQAKHKG------

Ig Domain < Tm <Cyt

Am TCRD : NPSVDADISKSTEECEAPTDQLIVRS------SEKVN----ILSMTVMSLRVLFAKSLAFNLLLSATFFLL------

Cm TCRD : ------PPQTS----V------

Cc TCRD : ERPTQNKCETNARTSASGFIDTG------VPKMN----FMNLLVTGLRILLAKCVAVNVMMTVKAFVF------

Dr TCRA : ------DEKVN----FLSLGIFWLRILFLKTVVFNVLVTFKAWMS------

Dr TCRB : ------KVKPK----GLKLVGFGYILFLSKSFLYALVVSAMFWKLKFSMKTKEIPEE------

Dr TCRD : NPVEDNPKKTATLECHTNTTTPPSTNND----DPKTN----SMTLLVIGLRILLAKCVAVNVMLSIKAFLF------

Dr TCRG : KPSVIKPSGGPDPTCPPSTETPISQESAN---PEKKQ----SLQLFVYGYAVMLMKNVLYFIVVFIVLLKRKAGKKEERS------

Dl TCRG : PPPTTSPSPPPSLPPSVQPSLIPSPPPSLIPAPPASAPSQYQVKLLCLMYTVLMVKSLVYCCGLSCLMIFRNKGPSTNCTHAD------

Gg TCRD : EEIKPANICNTTDTSAEDVK------MEKVN----MLSMAVLGLRVLLAKSIAFNTLMSIKLILF------

GcTCRD : TGPTQSSCAESSKDMMEAGNDPFQE------RPQAN----LLSLTLMGLRFLLFKSISVNLLMTARVWIS------

HfTCRD : PPKPIDCNKSSNGTSAGLNDTDND------LTEWN----FMSLTVMGLRVLFFKSVAFNVMMTARCCSFKEFSAMRWIQR------

Hs TCRD : TDHVKPKETENTKQPSKSCHKPKAIVH-----TEKVN----MMSLTVLGLRMLFAKTVAVNFLLTAKLFFL------

IpTCRD : CDQNSTLGISTSNNLTSPNKIKISG------DPKGN----TMLLIVTGLRLLLAKAVGINILMTIKAFLV------

IpTCRG : TSKSNAGVLNTCPTQKAEEAEEVEEEIMNFGVFEHSR----SLYLFSVTYVLLLVKNVLYFCTVFVLLFKRNPAKI------

Mm TCRD : FNNEKLPE------PENDT----QISEPCYGPRV-----TVHTEKVNMMS------

OvTCRD : SETTPKPTESENTTEIQVPATCYEPQVQ----PGKVN----MMSLSVLGLRMLFAKSVAVNFLFTAKLF------

Po TCRA : ------DPAVN----TVSLLVVALRLLFLKTVVFNVLLTLRLWLSHRV------

Po TCRB : DILTR------EKYLR----ITRQAKLSYSVLIIKSSVYGAFVAFLVWRLQSSTEKQN------

Po TCRD : ------NVRFN----SYLLLLNAARVMFTKIVAFTSVLTIRTLLA------

Po TCRG : PPPSTTPPSTPPPSETSP------EPTWS—QQQLKLLCLLYTVLMVKSLVYCCGLSLINILRNRERPPAAHKRTELPPAASTSH

Re TCRD : GVTPALDCDAGTSSNHTGTD------MPEVN----FMTLTVMGLRILFFKSVAFNVLMTARAWVF------

SsTCRD : NYNTTATTASVNCKDEPLNNVTTTNYTD----YTKMN----FTSLVVYGLRVLFAKAVAFNVLFTVKALVF------

ScTCRB : AVGDGMTR------DKYLR----ITQTAKLSYGVFIIKSCIYGAFVVFLVWRLQGSCGKQNN------

TrTCRD : QDTDQDTDQDTDQDTDQDTVQDTDQDTDQEWT------RSREN----LLLLRMDMLRVVLTKTVSLSTIVTIRALLL------

TnTCRD : ------PELWT----VTRRNLLLLQ------

XtTCRD : ------

Supplemental Figure 2: Multiple sequence alignment of selected C sequences. Created in Mega 6.0 using the Clustal W methodology. Ambystomamexicanum TCR delta (AY029365.1);Callorhinchusmilii TCR delta (NW_006897485 ); Cyprinuscarpio TCR delta (AB541473.2/BAI83002); Danio rerio TCR alpha (AF246178.1); Danio rerio TCR beta(NC_007128.6); Danio rerio TCR gamma (AY973943.1); DicentratchuslabraxTCR gamma (EU853841); Gallus gallus TCR delta (AF175433); Ginglymostomacirratum TCR delta(JF507709.1); Heterodontusfrancisci TCR delta (U22673); Homo sapiens TCR delta (A31326); Ictalurus punctatus TCR delta (HQ913590); Ictalurus punctatus TCR gamma (DQ435303); Mus musculus TCR delta (AAA51274); Ovisaries TCR delta (AJ290095.1); ParalichthysolivaceusTCR alpha (AB053227.1); ParalichthysolivaceusTCR beta (AB081559.1); Paralichthysolivaceus TCR delta (AU050806); Paralichthysolivaceus TCR gamma (AB081560.1); Salmo salarTCR delta (EF467299); Sinipercachuatsi TCR beta (EF596786.1); Takifugu rubripes TCR delta (AB222469); Tetraodon nigroviridis TCR delta (AJ410421.1); Xenopus tropicalis TCR delta (GQ262017.1).