Supporting Information
Allometric relationships to liver tissue concentrations of cyclic volatile methyl siloxanes in Atlantic cod
Authors: Nicholas A. Warner*,ǂ; Therese H. Nøstǂ,¥; Hector Andradeϩ; and Guttorm Christensenϩ
Affiliations:
ǂ NILU- Norwegian Institute for Air Research, FRAM Centre, Hjalmar Johansens gate 14, NO-9296 Tromsø, Norway
¥ Institute of Community Medicine, University of Tromø, N=-9019, Tromsø, Norway
ϩ Akvaplan-niva, FRAM Centre, Hjalmar Johansens gate 14, NO-9296 Tromsø, Norway
*Corresponding author: email:
Phone: (+47) 77 75 03 88
Fax: (+47) 77 75 03 76
Number of pages: 5
Number of Figures: 1
Number of Tables: 6
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Table S1. Atlantic cod sample information and liver concentration (ng/g wet weight (ww)) of cVMS, PCB 153 and PCB 180
Sample / Location / Date / Length (cm) / Weight (g) / Liver weight (g) / Age (years) / Condition (K) / % lipid / Concentration (ng/g ww)D4a / D5a / D6a / PCB 153 / PCB 180
Cod 1 / Nipøya / November, 2010 / 43 / 760 / 17.0 / 5 / 0.96 / 44.7 / 5.0 / 152 / 28.0 / 43.8 / 9.3
Cod 2 / Nipøya / November, 2010 / 51 / 1200 / 39.8 / 4 / 0.90 / 63.9 / 6.6 / 114 / 10.7 / 57.8 / 14.3
Cod 3 / Nipøya / November, 2010 / 66 / 2900 / 158.5 / 7 / 1.01 / 76.7 / 4.3 / 51.6 / 5.4 / 29.1 / 5.6
Cod 4 / Nipøya / November, 2010 / 56 / 1700 / 82.5 / 4 / 0.97 / 53.9 / < MDL / 37.4 / 4.7 / 16.2 / 3.4
Cod 5 / Nipøya / November, 2010 / 61 / 2000 / 59.2 / 6 / 0.88 / 47.6 / < MDL / 13.8 / 2.2 / 19.1 / 6.7
Cod 6 / Tromsøysund / November, 2010 / 46 / 960 / 15.2 / 6 / 0.99 / 56.8 / 33.4 / 480 / 66.5 / 139 / 53.2
Cod 7 / Tromsøysund / November, 2010 / 44 / 900 / 22.0 / 3 / 1.06 / 43.8 / 31.2 / 1110 / 61.0 / 96.9 / 34.8
Cod 8 / Tromsøysund / November, 2010 / 53 / 1600 / 74.1 / 6 / 1.07 / 71.7 / 45.6 / 671 / 53.4 / 70.9 / 15.7
Cod 9 / Tromsøysund / November, 2010 / 46 / 1200 / 20.5 / 5 / 1.23 / 32.8 / 21.0 / 349 / 24.9 / 108 / 38.0
Cod 10 / Tromsøysund / November, 2010 / 58 / 1900 / 63.4 / 6 / 0.97 / 59.1 / 9.3 / 200 / 17.0 / 42.8 / 16.7
Cod 11 / Tromsøysund / November, 2010 / 56 / 1600 / 24.6 / 5 / 0.91 / 34.7 / 13.4 / 493 / 26.8 / 126 / 33.4
Cod 12 / Tromsøysund / November, 2010 / 47 / 880 / 19.0 / 4 / 0.85 / 36.9 / 41.0 / 927 / 31.9 / 181 / 62.2
Cod 13 / Nipøya / April, 2011 / 46 / 1100 / 83.2 / 3 / 1.13 / 65.1 / 7.9 / 36.5 / 15.8 / 16.0 / 4.0
Cod 14 / Nipøya / April, 2011 / 51 / 1400 / 57.8 / 4 / 1.06 / 65.8 / 9.9 / 831 / 95.9 / 28.8 / 5.8
Cod 15 / Nipøya / April, 2011 / 52 / 1600 / 33.6 / 5 / 1.14 / 52.8 / 4.1 / 78.3 / 18.7 / 69.8 / 13.0
Cod 16 / Tromsøysund / April, 2011 / 49 / 1500 / 39.0 / 5 / 1.27 / 37.0 / 14.6 / 389 / 31.1 / 114 / 36.9
Cod 17 / Tromsøysund / April, 2011 / 51 / 1500 / 32.0 / 5 / 1.13 / 57.2 / 26.7 / 471 / 36.0 / 59.0 / 14.1
Cod 18 / Tromsøysund / April, 2011 / 56 / 2100 / 149.4 / 4 / 1.20 / 49.5 / 12.9 / 334 / 28.9 / 39.4 / 12.1
Cod 19 / Tromsøysund / April, 2011 / 54 / 1900 / 82.9 / 4 / 1.21 / 56.7 / 22.6 / 991 / 29.8 / 78.4 / 30.4
Cod 20 / Tromsøysund / April, 2011 / 54.5 / 1900 / 76.6 / 4 / 1.17 / 57.3 / 28.0 / 572 / 39.7 / 232 / 47.1
aMethod detection limits (MDL):2.4 ng/g ww (D4), 2.7 ng/g ww (D5), 1.7 ng/g ww (D6)
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Table S2A. Field blank concentrations (ng/g ww) for sediment and fish liver.
Location / Sediment field blank (ng/g ww) / Fish liver field blank (ng/g ww)D4 / D5 / D6 / D4 / D5 / D6
Tromsøya 2010 (Site A) / -0.3 / -0.1 / 0.1 / -1.3 / -0.2 / -1.3
Nipøya 2010 (Site B) / 0.1 / 0.1 / -0.2 / -0.4 / 0.5 / -0.1
Tromsø 2011 (Site A) / 0.8 / 0.1 / 0.3 / -0.5 / 0.4 / 0.1
Nipøya 2011 (Site B) / -0.5 / 0.1 / 0.1 / -0.8 / 0.3 / -0.4
a Negative concentrations are due to procedural blank correction (i.e., field blank contribution was less than procedural blank contribution).
Table S2B. Procedural blank concentrations for sediment and fish liver.
Matrix / Concentration (ng/g ww)D4 / D5 / D6
Sediment (n = 6) / 1.4 ± 0.1 / 0.4 ± 0.1 / 0.4 ± 0.1
Fish liver (n = 12) / 1.3 ± 0.2 / 0.4 ± 0.1 / 0.9 ± 0.5
a n represents number of procedural blanks
Table S3. Fortified sediment data for matrix detection limit (MDL) determination.
Concentration (ng/g ww)Sample ID / D4 / D5 / D6
Sediment MDL 1 / 3.2 / 3.0 / 2.9
Sediment MDL 2 / 3.6 / 3.7 / 3.3
Sediment MDL 3 / 3.0 / 2.9 / 2.6
Sediment MDL 4 / 2.4 / 2.0 / 2.1
Sediment MDL 5 / 1.9 / 2.5 / 2.7
Sediment MDL 6 / 3.2 / 3.4 / 3.0
Sediment MDL 7 / 3.8 / 4.0 / 3.6
Sediment MDL 8 / 4.1 / 3.7 / 3.4
Sediment MDL 9 / 4.3 / 4.0 / 3.7
Average / 3.3 / 3.3 / 3.0
Std.deviation / 0.8 / 0.7 / 0.50
MDL (ng/g ww)a / 2.3 / 2.0 / 1.4
a t-value: 2.896; Sediment fortification levels: 2.7 (D4), 3.2 (D5), and 2.4 (D6) ng/g ww
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Table S4. Fortified cod liver biota data for matrix detection limit (MDL) determination.
Concentration (ng/g ww)Sample ID / D4 / D5 / D6
Cod liver MDL 1 / 6.5 / 6.6 / 6.8
Cod liver MDL 2 / 6.4 / 7.0 / 6.7
Cod liver MDL 3 / 4.6 / 5.3 / 5.4
Cod liver MDL 4 / 3.8 / 4.7 / 4.8
Cod liver MDL 5 / 3.9 / 4.9 / 5.3
Cod liver MDL 6 / 6.2 / 7.8 / 6.7
Cod liver MDL 7 / 5.2 / 6.5 / 6.5
Cod liver MDL 8 / 4.3 / 5.2 / 5.5
Cod liver MDL 9 / 4.5 / 5.3 / 5.6
Cod liver MDL 10 / 5.0 / 5.5 / 6.1
Cod liver MDL 11 / 4.3 / 4.4 / 5.6
Cod liver MDL 12 / 5.4 / 6.8 / 6.6
Cod liver MDL 13 / 4.3 / 5.3 / 5.3
Cod liver MDL 14 / 4.8 / 5.8 / 5.8
Average / 5.0 / 5.8 / 5.9
Std.deviation / 0.9 / 1.0 / 0.6
MDL (ng/g ww)a / 2.4 / 2.7 / 1.7
a t-value: 2.718; Fish liver fortification levels: 2.7 (D4), 3.2 (D5), and 2.4 (D6) ng/g ww
Table S5. Sediment concentrations (ng/g wet weight (ww)) of cVMS detected at Tromsøysund (Site A)
Concentration (ng/g ww)D5 / D6
Sample ID / Location / 2010 / 2011 / 2010 / 2011
Sediment 1 / N 69⁰ 38' 53.9"; E 18⁰ 58' 25.0" / 9.3 / 7.7 / 1.4 / < MDL
Sediment 2 / N 69⁰ 40' 26.9"; E 18⁰ 59' 18.3" / 12.2 / 11.9 / 2.0 / 4.5
Sediment 3 / N 69⁰ 38' 45.3"; E 18⁰ 57' 55.2" / 8.7 / 3.5 / < MDL / < MDL
Average / 10.1 / 7.7 / 2.7 / 4.5
Std. deviation / 1.9 / 4.2 / 0.4 / -
aMethod detection limits (MDL):2.4 ng/g ww (D4), 2.7 ng/g ww (D5), 1.4 ng/g ww (D6). D4 was below MDL at Tromsøysund (Site A). No cVMS were detected at Nipøya (Site B)
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Figure S1: Correlation of fish weight and age with D5 concentration (ng/g lipid weight (lw)) from Tromsøysund
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