Alength: the Total Length of Each Specific EST Sequence

Table S4Database matching, gene discovery, and annotation of eight expressed sequence tag(EST) sequences

EST no. / Chr / Lengtha
(bp) / Geneb / Functional annotations of gene product
InterPro match / KEGG/GO mapping
E22 / 15 / 391 / titin-like / Domain:
Protein kinase domain (IPR000719);
Serine/threonine-/dual specificity protein kinase, catalytic domain (IPR002290);
Tyrosine-protein kinase, active site (IPR008266);
Tyrosine-protein kinase, catalytic domain (IPR020635);
Immunoglobulin subtype 2 (IPR003598);
Immunoglobulin subtype (IPR003599);
Immunoglobulin-like domain (IPR007110);
Immunoglobulin I-set (IPR013098);
Immunoglobulin-like fold (IPR013783);
Fibronectin, type III (IPR003961); / Biological Process:
protein phosphorylation (GO:0006468);
Molecular Function:
protein kinase activity(GO:0004672);
protein amino acid binding(GO:0005515);
protein tyrosine kinase activity(GO:0004713);
transferase activity, transferring phosphorus-containing groups(GO:0016772);
E36 / 18 / 689 / Interferon-related developmental regulator 1 (IFRD1),5’UTR / Domain:
Interferon-related developmental regulator, C-terminal (IPR006921);
Interferon-related developmental regulator, N-terminal (IPR007701);
Armadillo-like helical (IPR011989);
Armadillo-type fold (IPR016024); / Biological Process:
adult somatic muscle development(GO:0007527);
muscle cell differentiation(GO:0042692);
multicellular organismal development(GO:0007275);
Molecular Function:
binding(GO:0005488);
E42 / 14 / 621 / spacer region between myopalladin (MYPN) and protein atonal homolog 7-like / Un-known
E53 / 4 / 576 / glycogen debranching enzyme-like (GDE-like),3′UTR / Domain:
Six-hairpin glycosidase-like (IPR008928);
Family:
Amylo-alpha-1,6-glucosidase (IPR010401); / Biological Process:
glycogen biosynthetic process(GO:0005978);
Molecular Function:
Catalytic activity(GO:0003824);
amylo-alpha-1,6-glucosidase activity(GO:0004135);
E54 / ChrUScaf2204 / 662 / calpain 2, (m/II) large subunit (CAPN2) / Active Site:
Cysteine peptidase, cysteine active site (IPR000169);
Domain:
Peptidase C2, calpain, catalytic domain (IPR001300);
EF-hand domain (IPR002048);
Peptidase C2, calpain, domain III (IPR022683);
Family:
Peptidase C2, calpain family (IPR022684); / Biological Process:
proteolysis(GO:0006508);
Molecular Function:
calcium-dependent cysteine-type endopeptidase activity(GO:0004198);
calcium ion binding(GO:0005509);
Cellular Component:
intracellular(GO:0005622);
E82 / 17 / 604 / dynein, light chain, roadblock-type 1 (DYNLRB1) / Family:
Dynein light chain-related (IPR004942);
Dynein light chain, roadblock-type (IPR016561); / KEGG:
Tubulin-binding proteins (KEGG:ssc:100156707; KO:K10419);
Cellular Component:
centrosome (GO:0005813);
cytoplasmic dynein complex(GO:0005868);
E87 / 9 / 688 / crystallin, alpha B (CRYAB) / Domain:
Alpha crystallin/Heat shock protein (IPR001436);
Alpha crystallin/Hsp20 domain (IPR002068);
Alpha-crystallin, N-terminal (IPR003090);
HSP20-like chaperone (IPR008978);
Alpha-crystallin, subunit B (IPR012273); / KEGG:
Protein processing in endoplasmic reticulum (KEGG:ssc:100519789;PATH:ssc04141);
Biological Process:
muscle organ development (GO:0007517);
negative regulation of intracellular transport(GO:0032387);
E101 / 7 / 440 / X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3) / Family:
Kinesin light chain (IPR002151);
Domain：
Tetratricopeptide-like helical (IPR011990);
Tetratricopeptide repeat-containing domain (IPR013026);
Repeat:
TetratricopeptideTPR-1 (IPR001440);
Kinesin light chain repeat (IPR015792); / Molecular Function:
protein binding(GO:0005515);
microtubule motor activity(GO:0003777);
Cellular Component:
kinesin complex(GO:0005871);

aLength: The total length of each specific EST sequence

bGene: The eight ESTs were aligned to the specific and known pig genes by using the NCBIBLAST program