Instructions

1. Please complete and email this form together with the supporting materials
2. Our service representative will contact you with the quote

Customer Information

Name:
Phone:
Institution/Company:
Shipping address(needed to determine shipping cost):
Email:

ADCC Assay Service (SC1544)

Do You Need An Approved Monoclonal Antibody As Gold Standard?
Yes, I need. And I will provide the antibody.
Yes, I need. But I don’t have the antibody.
No, I don’t need.
Note: Rituximab(Rituxan)Trastuzumab (Herceptin), and Cetuximab (Erbitux) are available at GenScript.
Number of Test Antibodies:
Note: The gold standard would bealso included, if you choose it.
Nature of Test Antibodies:
Human；Mouse；Human & Mouse Chimeric；Fc fusion protein；Others
IgG1；IgG2；IgG3；Others
Name of Target Cell Lines:
Hot target cell line options:
(1) HER2+: SK-BR-3; BT-474; SK-OV-3; MCF-7;
MDA-MB-231; JIMT-1; T-47D; MDA-MB-453;
(2)CD20+: Daudi; Raji
(3) EGFR+: A431; A549
(4) tmTNFα+: immortalized tmTNFα+ stable cell line at GenScript
Others (please specify):
Note: There are over 250 tumor cell lines available at GenScript.Please contact us for detailed list.
Effector cell options:
Effector stable cell line (NK92 cells stably expressing FcRγ3A.158V/V)
Human peripheral blood lymphocytes (PBL)
Human blood NK cells purified from PBL
Other species
Mouse Rat
Optimization of E/T Ratios:
a. Antibody concentration: please specify: (μg/ml) (10μg/ml by default).
b. Number of E/T ratios:
Only one fixed E/T ratio. Please specify:.
PBL ADCC:(50:1, 25:1, and 10:1 by default).
NK92/ FcRγ3A.158V/V or blood NK ADCC: (10:1, 5:1, and 1:1 by default).
Dose-response Curve:
8 points (including 0) in triplicate.
Default starting concentration: 10 (μg/ml); dilution factor: 10 ;
Customized starting concentration: (μg/ml); dilution factor:
Detection of Antigen Expression Level of Target Cell(s) on FACS (Optional):
Yes, I need.
No, I don’t need.
Please specify any special requirements:

Project Information

Is this project for grant application purpose? Yes No
When will the project start?ImmediatelyWithin one monthWithin three monthsHalf a year later

Note: Final report includes protocol summary, plus concentration-response curve and EC50 value for each antibody.

CDC Assay Service (SC1545)

Do You Need An Approved Monoclonal Antibody As Gold Standard?
Yes, I need. And I will provide the antibody.
Yes, I need. But I don’t have the antibody.
No, I don’t need.
Note: Rituxan (Rituximab) is available at GenScript.
Number of Test Antibodies:
Note: The gold standard would be also included, if you choose it.
Nature of Test Antibodies:
Human；Mouse；Human & Mouse Chimeric；Fc fusion protein；Others
IgG1；IgG2；IgG3；Others
Name of Target Cell Lines:
Hot target cell line options:
(1) CD20+: Daudi; Raji
(2) tmTNFα+: immortalized tmTNFα+ stable cell line at GenScript
Others (please specify):
Note: There are over 250 tumor cell lines available at GenScript. Please contact us for detail list.
Dose-response Curve:
8 points (including 0) in triplicate.
Default starting concentration: 10 (μg/ml); dilution factor: 10 ;
Customized starting concentration: (μg/ml); dilution factor:
Please specify any special requirements:

Project Information

Is this project for grant application purpose? Yes No
When will the project start?ImmediatelyWithin one monthWithin three monthsHalf a year later

Note: Final report includes protocol summary, plus concentration-response curve and EC50 value for each antibody.