SUPPLEMENTARY TABLE SI.THE PLANT-POLYMER DEGRADING ENZYMES, PLANT GROWTH PROMOTING CHARACTERISTICS AND TOXONOMIC ASSIGNATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH P.laegivata and S. angustifoliaIN UNCONTAMINATED AND CONTAMINATED SITES

Strains / Taxonomic affiliation of 16S rRNA gene / Enzyme activity index (EI) / Index for
Siderophorea / % of P solubilizationb / IAAproductionc / N2-fixingd
Species / % Identity / Amylase / Xylanase / Cellulase / Pectinase
Natural hill
Sphaeralceaangustifolia
NE1E1g / Bacillus megaterium/aryabhattai / 99.6 / 2.00±0.00 / - / NG / NG / - / - / - / -
NE1E1p / Staphylococcus warneri/ pasteri / 96.0 / - / 1.32±0.06 / - / 1.19±0.05 / - / NG / - / -
NE1E2 / Staphylococcus epidermidis / 99.5 / - / - / NG / 1.55±0.00 / - / NG / - / -
NE1E3 / Microbacteriumarborescens / 95.9 / - / 1.33±0.00 / 1.33±0.00 / 1.25±0.19 / - / NG / + / -
NE1E7 / Microbacteriumarborescens / 97.2 / - / - / - / NG / - / NG / + / +
NE2E1 / Micrococcus luteus / 97.9 / - / 1.44±0.19 / - / - / - / - / - / -
NE2E2 / Microbacteriumschleiferi / 97.7 / - / NG / 1.00±0.00 / NG / - / NG / - / -
NE2E3 / Microbacteriumoxydans / 98.4 / - / 2.00±0.00 / 4.00±0.00 / NG / - / NG / - / -
Prosopislaegivata
NM1E1 / Nocardiopsisdassonvillei / 99.7 / 7.00±1.00 / 1.42±0.14 / 1.30±0.05 / 1.55±0.18 / - / - / - / NG
NM1E2 / Bacillusaxarquiensis/ malacitensis / 97.6 / 1.50±0.00 / - / NG / - / - / - / - / NG
NM1E3 / Bacillusaxarquiensis/ subtilis / 97.9 / 1.97±0.06 / 1.20±0.25 / 1.50±0.00 / 1.17±0.06 / 1.20±0.00 / - / NG / +
NM1E4 / Microbacteriumschleiferi / 97.9 / 1.10±0.00 / 1.17±0.16 / 2.00±0.00 / 1.00±0.00 / - / - / - / -
NM1E5 / Bacillus malacitensis/ axarquiensis / 98.9 / 1.00±0.00 / 1.44±0.19 / 3.00±0.00 / 1.10±0.00 / - / 33.00±0.00 / + / -
NM2E1 / Micrococcus luteus / 98.9 / - / NG / NG / NG / NG / NG / NG / -
NM2E2 / Pseudomonas stutzeri / 98.5 / - / NG / - / NG / 1.12±0.10 / - / - / -
NM2E3 / Brevibacterium salitolerans / 95.8 / - / - / - / - / 1.20±0.00 / - / - / +
NM2E4 / Pseudomonas stutzeri / 98.4 / 2.00±0.00 / - / - / NG / 1.27±0.12 / NG / - / -
NM2E5 / Bacillus megaterium/aryabhattai / 98.6 / 1.30±0.08 / 1.50±0.26 / 1.06±0.06 / - / - / 40.00±0.00 / + / -
NM2E6 / Bacillus simplex / 97.6 / - / NG / NG / NG / NG / NG / NG / -
NM2E7 / Pseudomonas stutzeri / 99.9 / - / - / - / NG / - / NG / - / -
NM2E8 / Bacillus malacitensis/axarquiensis/subtilis / 99.0 / 1.90±0.15 / 1.18±0.03 / 1.33±0.00 / 1.20±0.04 / - / - / - / -
NM2E9 / Bacillus malacitensis/axarquiensis / 98.4 / 1.60±0.29 / - / 1.72±0.26 / 1.60±0.15 / - / - / - / +
NM2E10 / Kocuriarhizophila / 97.7 / - / NG / NG / NG / NG / NG / NG / -
NM2E11 / Bacillus cereus / 99.3 / 1.10±0.00 / - / - / - / - / 12.05±0.00 / - / -
NM2E12 / Bacillusmalacitensis/axarquiensis / 98.9 / 1.30±0.00 / 1.60±0.18 / 1.00±0.00 / - / - / - / - / -
NM2E13 / Leucobacteraridicollis / 98.7 / 3.00±0.00 / 1.31±0.10 / 1.00±0.00 / 1.17±0.112 / - / - / - / +
NM2E14 / Bacillusmalacitensis/axarquiensis / 97.0 / 1.30±0.00 / - / 1.87±0.20 / NG / - / 70.00±17.32 / - / -
NM2E15 / Bacillusmalacitensis /axarquiensis / 99.5 / 1.50±0.00 / 1.50±0.00 / 1.87±0.20 / 1.18±0.06 / - / - / - / -
NM2E16 / Bacillusendophyticus / 99.4 / - / 1.50±0.00 / NG / 0.75±0.00 / - / - / - / -
NM2E17 / Bacillusmalacitensis/axarquiensis / 99.7 / 3.50±0.45 / 1.32±0.07 / - / 1.17±0.06 / 1.72±0.092 / - / - / -
NM2E18 / Bacillus megaterium/ aryabhattai / 99.5 / 1.20±0.00 / 1.27±0.03 / 1.50±0.99 / 1.37±0.06 / - / - / + / -
NM3E1 / Leucobacteraridicollis / 98.7 / - / NG / NG / NG / NG / NG / NG / -
NM3E2 / Arthrobacterscleromae / 98.9 / 1.00±0.00 / - / 1.25±0.00 / - / - / - / - / -
NM3E3 / Bacillusaryabhattai / 99.9 / 1.20±0.07 / - / - / - / - / 31.00±10.14 / + / -
NM3E5 / Staphylococcussaprophyticus / 99.9 / 1.33±0.29 / - / - / NG / - / - / NG / NG
NM3E6 / Bacillusendophyticus / 99.4 / 1.33±0.57 / 1.53±0.06 / 1.50±0.00 / - / - / 45.00±18.02 / - / -
NM3E7 / Bacillus malacitensis/axarquiensis / 98.5 / - / NG / NG / NG / 1.29±0.01 / - / - / -
NM3E9 / Bacillus megaterium/aryabhattai / 98.8 / 1.40±0.00 / - / 1.49±0.16 / 1.50±0.00 / - / 17.30±9.29 / - / +
NM3E10 / Bacilluscereus / 95.0 / 2.20±0.35 / 1.53±0.06 / - / 1.80±0.00 / - / - / + / -
NM3E11 / Bacilluscereus / 97.5 / 1.32±0.16 / 1.25±0.00 / 3.50±0.87 / NG / - / NG / - / -
Mine tailing
Sphaeralcea angustifolia
CE1E1 / Pseudomonasstutzeri / 99.7 / 1.50±0.00 / - / - / NG / - / - / - / +
CE2E1 / Arthrobacterscleromae / 98.1 / 1.39±0.10 / - / 1.50±0.00 / 1.38±0.10 / - / - / - / NG
CE3E1 / Staphylococcus warneri/pasteri / 99.9 / - / - / - / 1.36±0.13 / 1.40±0.00 / NG / - / NG
CE3E2 / Bacillusmalacitensis/axarquiensis / 98.2 / 2.00±0.00 / 1.46±0.06 / - / 1.30±0.10 / - / - / - / +
CE3E3 / Bacillus malacitensis/axarquiensis / 99.0 / 2.83±0.29 / - / - / 1.33±0.06 / 0.75±0.00 / 11.08±4.83 / - / -
CE3E4 / Staphylococcus warneri/pasteri / 98.0 / - / - / - / 1.33±0.00 / 1.20±0.00 / - / - / -
CE3E5 / Staphylococcus warneri/pasteri / 99.0 / - / - / - / 1.33±0.00 / 1.40±0.00 / NG / - / -
Prosopislaegivata
CM1E1 / Kocuriarhizophila / 96.1 / - / 1.44±0.21 / 1.50±0.00 / 1.67±0.29 / - / - / - / -
CM1E2 / Bacillusvallismortis / 98.9 / 1.60±0.17 / 1.60±0.00 / - / 1.37±0.15 / - / - / - / -
CM1E3 / Bacillusvallismortis / 99.4 / 1.57±0.06 / 1.83±0.29 / - / 1.60±0.50 / - / - / - / -
CM1E4 / Bacillusendophyticus / 99.0 / NG / NG / NG / NG / - / - / - / -
CM1E5 / Bacillusniacini / 97.3 / 2.00±0.00 / - / - / NG / - / - / - / NG
CM1E6 / Bacillusvallismortis / 99.8 / 1.70±0.00 / 1.27±0.02 / 1.00±0.07 / 1.80±0.02 / - / - / - / -
CM1E7 / Bacillusatrophaeus / 98.1 / - / 1.55±0.09 / - / 1.75±0.53 / - / - / - / -
CM1E8 / Microbacteriumarborescens / 98.5 / 2.50±0.00 / 1.00±0.00 / - / NG / - / - / - / NG
CM2E1 / Staphylococcus warneri/pasteri / 98.4 / 1.00±0.00 / - / NG / 1.28±0.05 / - / NG / - / -
CM2E2 / Bacillus endophyticus / 99.7 / - / 1.55±0.19 / NG / NG / - / - / - / -
CM2E3 / Bacillusendophyticus / 98.5 / - / 1.80±0.18 / - / - / - / - / - / -
CM2E4 / Staphylococcus warneri/pasteri / 98.4 / 1.00±0.00 / - / NG / 1.20±0.11 / 3.89±0.20 / - / - / -
CM2E4c / Staphylococcus warneri/pasteri / 99.6 / - / NG / NG / - / - / - / - / NG

a Average (±, stantarddesviation) of the Enzymatic Index (EI) or Production Index (PI).

b% phosphate solubilization.

c IAA production: -, no able to produce IAA.

d Nitrogen-fixing assay: - no able to growth in nitrogen-free medium.

NG, no growth.

SUPPLEMENTARY TABLE SII. THE PHYSIOLOGICAL CHARACTERIZATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH P. laegivata and S. angustifoliaIN UNCONTAMINATED AND CONTAMINATED SITES.+, GROWTH; -, NO GROWTH

Strains / pH / Salinity (%)
4.5 / 5 / 6 / 8 / 9 / 10 / 11 / 12 / 13 / 1 / 3 / 5 / 7 / 9 / 11 / 13 / 15 / 20
Natural Hill
Spharealcea angustifolia
NE1E1g Bacillus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
NE1E1p Staphylococcus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NE1E2 Staphylococusepidermidis / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
NE1E3 Microbacteriumarborescens / - / - / - / + / + / + / + / + / - / + / + / + / + / - / - / - / - / -
NE1E7 Microbacteriumarborescens / - / - / + / + / + / + / + / - / - / + / + / + / + / + / - / - / - / -
NE2E1 Micrococcus luteus / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / - / - / -
NE2E2 Microbacteriumschleiferi / - / - / + / + / + / + / + / + / - / + / + / + / - / - / - / - / - / -
NE2E3 Microbacteriumoxydans / - / + / + / + / + / + / + / + / - / + / + / + / + / + / + / - / - / -
Prosopislaegivata
NM1E1 Nocardiopsisdassonvillei / - / - / - / + / + / + / + / - / - / + / + / - / - / - / - / - / - / -
NM1E2 Bacillussp / - / + / + / + / + / + / + / + / - / + / + / + / + / + / + / - / - / -
NM1E3 Bacillussp / - / - / + / + / + / + / + / + / - / + / + / + / + / + / + / - / - / -
NM1E4 Microbacteriumschleiferi / - / - / + / + / + / + / + / + / - / + / + / + / + / + / + / - / - / -
NM1E5 Bacillus sp / + / - / + / + / + / + / - / - / - / + / + / + / + / + / + / - / - / -
NM2E1 Micrococcus luteus / - / - / + / + / + / + / + / + / + / + / + / + / + / + / - / - / - / -
NM2E2 Pseudomonas stutzeri / - / - / - / + / + / + / + / + / + / + / + / + / + / + / - / - / - / -
NM2E3 Brevibacterium sp / - / - / - / + / + / + / + / + / - / + / + / + / + / - / - / - / - / -
NM2E4 Pseudomonas stutzeri / - / - / - / + / + / + / + / + / + / + / + / + / + / + / - / - / - / -
NM2E5 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E6 Bacillus simplex / - / - / + / + / + / + / - / - / - / + / + / + / + / + / - / - / - / -
NM2E7 Pseudomonas stutzeri / - / - / + / + / + / + / + / - / - / + / + / + / + / - / - / - / - / -
NM2E8 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E9 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E10 Kocuria sp / - / + / + / + / + / + / - / - / - / + / + / + / + / - / - / - / - / -
NM2E11 Bacillus cereus / - / + / + / + / + / + / + / - / - / + / + / + / + / + / - / - / - / -
NM2E12 Leucobacteraridicollis / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E13 Bacillus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / - / -
NM2E14 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E15 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / + / -
NM2E16 Bacillusendophyticus / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E17 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM2E18 Bacillus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
NM3E1 Leucobacteraridicollis / - / + / + / + / + / + / + / - / - / + / + / + / + / - / - / - / - / -
NM3E2 Arthrobacterscleromae / - / - / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / -
NM3E3 Bacillusaryabhattai / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / - / - / -
NM3E5 St. Saprophyticus / - / - / + / + / + / + / + / - / + / + / + / + / - / - / - / - / - / -
NM3E6 Bacillusendophyticus / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / - / -
NM3E7 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / - / - / -
NM3E9 Bacillus sp / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
NM3E10 Bacilluscereus / - / + / + / + / + / + / + / - / - / + / + / + / + / + / - / - / - / -
NM3E11 Bacilluscereus / - / - / - / + / + / + / + / + / - / + / + / + / - / - / - / - / - / -
Mine tailing
Sphaeralcea angustifolia
CE1E1 Pseudomonas stutzeri / - / - / - / + / - / - / - / - / - / + / + / + / + / - / - / - / - / -
CE2E1 Arthrobacterscleromae / - / - / + / + / + / + / + / - / - / + / + / + / + / - / - / - / - / -
CE3E1 Staphylococcus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
CE3E2 Bacillus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / - / -
CE3E3 Bacillus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / - / - / - / -
CE3E4 Staphylococcus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / +
CE3E5 Staphylococcus sp / - / + / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / +
Prosopislaegivata
CM1E1 Kocuriasp / - / + / + / + / + / + / - / - / - / + / + / + / + / + / + / - / - / -
CM1E2 Bacillusvallismortis / - / + / + / + / + / + / - / - / - / + / + / + / + / + / + / - / - / -
CM1E3 Bacillusvallismortis / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / - / - / -
CM1E4 Bacillusendophyticus / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
CM1E5 Bacillusniacini / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
CM1E6 Bacillusvallismortis / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
CM1E7 Bacillusatrophaeus / - / - / + / + / + / + / - / - / - / + / + / + / + / + / + / + / - / -
CM1E8 Microbacteriumarborescens / - / - / - / + / - / - / - / - / - / - / - / - / - / - / - / - / - / -
CM2E1 Staphylococcus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
CM2E2 Bacillus endophyticus / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / - / -
CM2E3 Bacillus endophyticus / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / - / - / -
CM2E4 Staphylococcus sp / - / - / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
CM2E4C Staphylococcus sp / - / + / + / + / + / + / + / - / - / + / + / + / + / + / + / + / + / -
Number of resistantstrains / 1 / 10 / 52 / 60 / 58 / 58 / 38 / 13 / 5 / 59 / 59 / 58 / 55 / 48 / 40 / 28 / 11 / 2
% of resistantstrains / 1.6 / 17 / 87 / 100 / 97 / 97 / 63 / 22 / 8 / 98 / 98 / 97 / 92 / 80 / 67 / 47 / 18 / 3.3