Acrylamide mix ( Acrylamide and bisacrylamide)

TEMED(N,N,N’,N;-tetramethylethylnediamine)

Sodium Dodecyl sulfate (SDS)Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Dr.Robert Insall / Assessment Number* / 2032
Signature
Assessor / Soha Zouwail / Date of Assessment / 17/2/02

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / 8th Floor labs
2 / PERSONS WHO MAY BE AT RISK
List names where possible / Frank Zimmerman,Jason King , Margarita Tchibalina,Robert Insall ,Soha Zouwail, and may be other members of both Robert’s &Laura’s labs
3 / ACTIVITY ASSESSED / Use of SDS -Page gel for protein.
MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT** / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Acrylamide mix
( Acrylamide and bisacrylamide) / 4 ml of 30% Acrylamide mix / Toxic&
Carcinogenic / R39/27Very toxic:danger of irreversible effects in contact with skin
R23/24/25 Toxic by inhalation ,in contact with the skin &if swallowed
R45 may cause cancer
R46 may cause heritable genetic damage
R 46 Danger of serious damage to health by prolonged exposure
TEMED(N,N,N’,N;-tetramethylethyl
nediamine) / 4 ul / Irritant / R11 highly flammable
R20/22 harmful by inhalation or if swallowed
R34 causes burns
R 36/37/38 Irritant to eyes ,respiratory system &skin
Sodium Dodecyl sulfate (SDS) / 0.1 ml of 10% solution / Irritant
Harmful if swallowed / R41 risk of serious damage to eyes
R42 may cause sensitisation by inhalation.
R20/22 harmful by inhalation &if swallowed
R36/37/38 irritation to eyes, respiratory system, &skin.
Ammonium persulphate (APS) / 0.1 ml of 10% APS / Irritant
Harmful if swallowed / R8 contact with combustible material may cause fire.
R22 harmful if swallowed.
R34 causes burns.
R42/43 may cause sensitisation by inhalation or skin contact.
Hydroxymethylaminomethane(Tris) / 2.5 ml of 1.5 M Tris / Irritant / R36/37/38 Irritant to eyes, respiratory system, &skin.
5 / INTENDED USE
Give brief details and attach protocol/instructions / Preparation & use of gels containing different percentages of acrylamide to separate proteins of different sizes.
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
There is risk of inhalation of powder when preparing SDS solution. There is risk of skin contamination when using acrylamide mix, or skin burn when using the TEMED & APS solutions.
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The level of risk is not acceptable and control measures should be followed.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
The sodium dodecyl sulphate (SDS) should be weighed in the fume hood on the 7th floor whilst wearing mask, gloves and lab coat.
The acrylamide mix &TEMED should be all used while wearing gloves.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Personnel involved in the clean up procedure must be adequately trained and experienced in handling carcinogenic materials.
10 / MONITORING
Performance of control measures,
Check fume hood is working properly before use.
Personal exposure / Health Surveillance
Wearing lab coat and gloves during the whole procedure.
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Polymerised Acrylamide can be placed in yellow bags for incineration. Any of the other chemicals could be disposed in the drain followed by large amount of water.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
February 2007
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Any spillage of acrylamide should be carefully cleaned whilst wearing gloves and protective clothes and the waste should be discarded as special waste (contact Brian Facer in stores). Treat contaminated surface with household bleach and then wash thoroughly with water. If APS or TEMED has been spilled, sources of ignition and combustible material should be eliminated & absorbant material should be spread on the spill.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
If any of the hazardous chemicals comes in contact with the skin, remove people from source of contamination, bring injured person under a shower, wash contaminated part of the body for a minimum of 10 minutes, remove any contaminated clothing and seek medical advice. Ensure that the person involved in the clean up is wearing gloves and lab coat. Breathing apparatus may also be required.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
For air contamination: evacuate the place and ventilate room as much as possible to dispel vapour.
For flammable materials: shut off all sources of ignition in the room, evacuate all people immediately, contact security or ring fire alarm.
CONTACT PHONE

10.10.00

* Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.