APPENDIX 1.

Comparative genomic analysis of three strains of S. zooepidemicus and L. lactis MG1363 for the presence of genes of the HA biosynthetic pathway (MW- molecular mass, pI- isoelectric point, CSD- coding sequence , bp- basepair, AA- amino acid residue)

Gene / Accession / Enzyme / EC. No / MW / pI / CDS
(bp) / AA
Streptococcus equi subsp zooepidemicus H70 (GenBank accession - FM204884)
hasA / YP_002743732 / Hyaluronan synthase / 2.4.1.212 / 47.8 / 8.78 / 1253 / 417
hasB / YP_002743733 / UDP-glucose dehydrogenase / 1.1.1.22 / 45 / 5.09 / 1205 / 401
hasC.1 / YP_002743734 / UDP-glucose pyrophosphorylase.1 / 2.7.7.9 / 33.2 / 5.94 / 902 / 300
hasD
(glmU) / YP_002743735 / Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.157
2.7.7.23 / 49.4 / 5.55 / 1380 / 459
hasE
(pgi) / YP_002743736 / Phosphoglucoisomerase / 5.3.1.9 / 49.5 / 4.88 / 1349 / 449
GlcK / YP_002744918 / Glucokinase / 2.7.1.2 / 33.6 / 4.75 / 972 / 323
pgmA / YP_002744896 / Phosphoglucomutase / 5.4.2.2 / 63.2 / 4.99 / 1695 / 564
glmS / YP_002744607 / Glucosamine-fructose-6-phosphate aminotransferase / 2.6.1.16 / 65.4 / 5.20 / 1812 / 603
glmM / YP_002744292 / Phosphoglucosamine mutase / 5.4.2.10 / 48.4 / 4.74 / 1353 / 450
hasC.2 / YP_002745261 / UTP-glucose-1-phosphate uridylyltransferase 2 / 2.7.7.9 / 33.4 / 5.78 / 909 / 302
glmU / YP_002744034 / Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.157
2.7.7.23 / 49.4 / 5.62 / 1383 / 460
Streptococcus equi subsp zooepidemicus MGCS10565 (GenBank accession - CP001129)
hasA / YP_002122590 / Hyaluronan synthase / 2.4.1.212 / 47.8 / 8.90 / 1254 / 417
hasB / YP_002122591 / UDP-glucose dehydrogenase / 1.1.1.22 / 45 / 5.09 / 1206 / 401
hasC.1 / YP_002122592 / UDP-glucose pyrophosphorylase 1 / 2.7.7.9 / 33.3 / 6.13 / 903 / 300
glmU / YP_002122593 / Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.157
2.7.7.23 / 49.3 / 5.70 / 1380 / 459
Pgi / YP_002122594 / Phosphoglucoisomerase / 5.3.1.9 / 49.5 / 4.88 / 1350 / 449
glcK / YP_002122969 / Glucokinase / 2.7.1.2 / 33.6 / 4.75 / 972 / 323
pgmA / YP_002123313 / Phosphoglucomutase / 5.4.2.2 / 64 / 5.01 / 1749 / 582
glmS / YP_002123255 / Glucosamine-fructose-6-phosphate aminotransferase / 2.6.1.16 / 65.4 / 5.21 / 1812 / 603
glmM / YP_002123566 / Phosphoglucosamine mutase / 5.4.2.10 / 48.3 / 4.68 / 1353 / 450
hasC.2 / YP_002122607 / UDP-glucose pyrophosphorylase / 2.7.7.9 / 35.7 / 5.89 / 969 / 322
Streptococcus equi subsp zooepidemicus 4047 (GenBank accession - FM204883)
hasA / YP_002745678 / Hyaluronan synthase / 2.4.1.212 / 47.8 / 8.90 / 1254 / 417
hasB / YP_002745679 / UDP-glucose dehydrogenase / 1.1.1.22 / 45 / 5.09 / 1206 / 401
hasC.1 / YP_002745680 / UDP-glucose pyrophosphorylase / 2.7.7.9 / 33.4 / 5.64 / 909 / 302
glmU / YP_002745691 / Bifunctional N-acetyl glucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.157
2.7.7.23 / 49.5 / 5.48 / 1383 / 460
Pgi / YP_002745690 / Glucose-6-Phosphate isomerase / 5.3.1.9 / 49.5 / 4.88 / 1350 / 449
glcK / YP_002745969 / glucokinase / 2.7.1.2 / 33.6 / 4.75 / 972 / 323
pgmA / YP_002745991 / Phosphoglucomutase / 5.4.2.2 / 63.2 / 4.93 / 1695 / 564
glmS / YP_002746335 / Glucosamine-fructose-6-phosphate aminotransferase / 2.6.1.16 / 65.4 / 5.2 / 1812 / 603
glmM / YP_002746668 / Phosphoglucosamine mutase / 5.4.2.10 / 48.4 / 4.71 / 1353 / 450
hasC.2 / YP_002745692 / UDP-glucose pyrophosphorylase / 2.7.7.9 / 33.3 / 5.94 / 903 / 300
glmU / YP_002746909 / Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.157
2.7.7.23 / 49.4 / 5.55 / 1383 / 460
Lactococcus lactis MG1363 ( GenBank accession - AM406671)
glck / YP_001033545 / Glucokinase / 2.7.1.2 / 33.8 / 4.76 / 972 / 323
Ugd / YP_001032899 / UDP-glucose dehydrogenase / 1.1.1.22 / 43.4 / 5.9 / 1170 / 389
galU / YP_001032427 / UDP-glucose pyrophosphoryase / 2.7.7.9 / 35.0 / 5.62 / 943 / 313
glmU / YP_001033331 / Bifunctional N-acetylglucosamine-1-phosphate uridyl transferase / glucosamine-1-phosphate acetyl transferase / 2.3.1.15
2.7.7.23 / 49.1 / 5.99 / 1377 / 458
Pgi / YP_001033688 / Glucose-6-Phosphate isomerase / 5.3.1.9 / 49.6 / 4.89 / 1347 / 448
glmM / YP_001031801 / Phosphoglucosamine mutase / 5.4.2.10 / 48.7 / 4.79 / 1359 / 452
pgmA / YP_001031890 / α-Phosphoglucomutase / 5.4.2.2 / 28.3 / 4.57 / 759 / 252
glmS / YP_001032803 / Glucosamine-fructose-6-phosphate aminotransferase / 2.6.1.16 / 65.8 / 5.02 / 1818 / 605

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