Standard Operating Procedures

Laboratory Specific

Chemical: URACIL MUSTARD
aka 2,4 (1H,2H)-Pyrimidinedione or Uramustine

Please fill out the form completely. Print a copy and insert into your

Laboratory Safety Manual and Chemical Hygiene Plan.

Refer to instructions for assistance.

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Department:______Date when SOP was written:______

Date when SOP was approved by the lab supervisor: ______

Principal Investigator:______

Internal Laboratory Safety Coordinator/Lab Manager:______

Laboratory Phone:______Office Phone:______

Emergency Contact:______

(Name and Phone Number)

Location(s) covered by this SOP:______

(Building/Room Number)

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Type of SOP: Process Hazardous Chemical Hazardous Class

Purpose

Uracil Mustard is a chemotherapy drug which belongs to the class of alkylating agents. It is used for its antineoplastic properties. It is used in lymphatic malignancies such as non-Hodgkin's lymphoma. It works by damaging DNA, primarily in cancer cells that preferentially take up the uracil due to their need to make nucleic acids during their rapid cycles of cell division. At high concentrations of the drug, cellular RNA and protein synthesis are also suppressed. The DNA damage leads to apoptosis of the affected cells. Chemically it is a derivative of nitrogen mustard and uracil.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 66-75-1

Class: Carcinogen, Highly Toxic by ingestion, Irritant!!

Molecular formula: C8H11Cl2N3O2

Boiling Point: N/A

Melting Point: 206.1 ° C

Potential Hazards/Toxicity

EMERGENCY OVERVIEW:

Danger! The major hazards encountered in the use and handling of uracil mustard stem from its toxicological properties. Exposure to this odorless, off-white, crystalline powder may occur from its manufacture, formulation, or distribution for use as an antineoplastic drug. Effects from exposure may include nausea, vomiting, diarrhea, dermatitis, irritability, depression, leukopenia, thrombocytopenia, and anemia. Uracil mustard has been indicated as possibly carcinogenic to humans. Hazardous decomposition products formed under fire conditions. - Carbon oxides, nitrogen oxides (NOx), Hydrogen chloride gas.

Potential Health Effects:

Eye: There is some evidence to suggest that this material can cause eye irritation and damage in some persons.

Skin: Skin contact with the material may initially produce irritation. Systemic effects may result following absorption and these may be fatal. Avoid contact with skin.

Ingestion: Severely toxic effects including fatality may result if ingested. Effects associated with their use include increased heart rate, decreased saliva production and other secretions and reduction in bowel movements. Ingestion LD50 for rats is 3.55mg/kg.

Inhalation: Highly toxic by inhalation. Avoid dust formation. Inhalation of dusts, or fume, especially for prolonged periods, may produce respiratory discomfort and occasionally, distress. Persons with impaired respiratory function, airway diseases and conditions such as emphysema or chronic bronchitis, may incur further disability if excessive concentrations of particulate are inhaled. Hazardous decomposition products formed under fire conditions are Carbon oxides, nitrogen oxides (NOx), Hydrogen chloride gas.

Personal Protective Equipment (PPE)

Eyes: Safety goggles should be worn to minimize the possibility of liquid of vapor or dust eye contact.

Skin: Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.

Clothing: Employees should be provided with and required to use impervious clothing, gloves, and safety glasses. Employees should wear long pants, closed toe shoes and a lab coat at all times.

Respirators: Wear respiratory protection is required. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Where risk assessment shows air-purifying respirators are appropriate use a dust mask type N95 (US) or type P1 (EN 143) respirator. Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N99 (US) or type P2 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).

Engineering Controls

Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use only in a chemical fume hood. Make sure to wear splash goggles, a lab coat, gloves and the appropriate respirator. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed. Normal measures for preventive fire protection.

First Aid Procedures

Eyes: In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Do not use an ointment. Seek medical attention.

Skin: In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid immediately. Wash clothing before reuse.

Ingestion: Call the poison control center at 1-800-222-1222. If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid.

Inhalation: POISON chemical! If inhaled, get medical aid immediately. Remove victim to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.

Notes to Physician: Treat symptomatically and supportively.

Special Handling and Storage Requirements

Handling: Wear protective splash goggles, respirator, gloves and lab attire at all times while handling this chemical. Use only in a fume hood. This chemical is known to be carcinogenic. It is stable under recommended storage conditions.

Storage: Uracil mustard should be stored and transported in air-tight glass bottles or ampoules which are, in turn, placed inside strong screw-cap or snap-top containers. Empty drums should be decontaminated; never re-use containers. Keep in a well-ventilated room.

Spill and Accident Procedure

Chemical Spill Dial 911 and x59797

Spill – Spills of uracil mustard should be taken up with a wet mop or vacuum cleaner equipped with a high efficiency particulate filter on the exhaust. Do not sweep, brush, or use any other method which may disperse the substance. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions. Remove all sources of ignition. Use a spark-proof tool. If the material is dry, explosives experts may be necessary to dispose of the spill. Provide ventilation.

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Medical Emergency Dial 911 or x52111

Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.

Decontamination/Waste Disposal Procedure

Waste disposal procedures

Dispose of waste as Hazardous Waste in the appropriate manner as recommended by the manufacturer.

Material Safety Data Sheet (MSDS) Location

(State the location of MSDS)

Hardcopy or electronic copy must be available.

Online MSDS can be accessed at http://msds.ehs.ucla.edu.

Protocol/Procedure

(Add specific description of procedure.)

Note: Any deviation from this SOP requires written approval from PI.

Documentation of Training (signature of all users is required)

I have read and understand the content of this SOP:

Name Signature Date

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