Javier A. Santander M.
Date of Birth: March 24, 1975
Email: ;
Skype: jasantanderm
Phone: 1-709-8643268 (office)
Mobile: 1-709-6934262
Current Address
Memorial University of Newfoundland,
Department of Ocean Sciences, Ocean Science Centre
Marine Microbial Pathogenesis and Vaccinology Lab AX-3005
0 Marine Lab Road, St. John’s, A1C 5S7, NL Canada
1
Javier Santander, Curriculum Vitae
EDUCATION
2012 Ph.D. Microbiology, Arizona State University, Center for Infectious Diseases and Vaccinology, Biodesign Institute and School of Life Sciences, Tempe, Arizona. USA. “Studies of Conserved Genes in Edwardsiella ictaluri to Develop a Universal Recombinant Attenuated Vaccine Platform for the Aquaculture Industry”. Tutor: Roy Curtiss III, Ph.D.
2003 M.Sc. Microbiological Sciences. Pontificia Universidad Católica de Valparaíso, Institute of Biology, Valparaíso, Chile. “Isolation and Characterization of Lytic Bacteriophages Against Salmonella Enteritids and its Evaluation In Vivo in Chaenorhabditis elegans Host”. Tutor: James Robeson, Ph.D.
2000 Marine Biologist. Universidad Católica del Norte, School of Marine Biology, Center for Marine Research, Coquimbo, Chile. “Microbial Interactions in the Microalgae Culture of Isochysis galabana”. Tutor: Claudio Miranda, Ph.D
1997 Bachelor in Sea Sciences. Universidad Católica del Norte, Center for Marine Research, Coquimbo, Chile.
Language
Spanish: native language
English: second language
professional CAREER
2016- present Assistant Professor, Memorial University of Newfoundland, Department of Ocean Sciences, Canada
2016- present Adjunct Professor, Center for Genomic and Bioinformatics, Faculty of Sciences, University Mayor, Huechuraba, Chile
2013- 16 Adjunct Professor, Arizona State University, School of Life Sciences, Tempe, USA.
2013- 16 Director Animal Facility, Center for Genomics and Bioinformatics, Faculty of Sciences, University Mayor, Chile
2013- 16 Assistant Professor, Center for Genomic and Bioinformatics, Faculty of Sciences, University Mayor, Huechuraba, Chile
2012-13 Assistant Research Scientist. Arizona State University, Center for Infectious Diseases and Vaccinology, Biodesign Institute, Tempe, USA.
2010-12 Teaching Assistant, Arizona State University, School of Life Sciences, Tempe, USA.
2007-12 Graduate Research Associate, Arizona State University, Center for Infectious Diseases and Vaccinology, Biodesign Institute and School of Life Sciences, Tempe, USA.
2005-12 Assistant Research Technologist, Arizona State University, Center for Infectious Diseases and Vaccinology, Biodesign Institute, Tempe, USA.
2004-05 Senior Researcher, Washington University in St. Louis, Institute of Biology. St. Louis, Missouri, USA.
2003-04 Teaching Assistant, Universidad Andrés Bello, Institute of Health, Viña del Mar. Chile.
2001-04 Assistant Researcher, Pontificia Universidad Católica de Valparaíso, Institute of Biology, Valparaíso, Chile.
2000-04 Graduate Researcher, Pontificia Universidad Católica de Valparaíso, Institute of Biology, Valparaíso, Chile.
1996-99 Laboratory Instructor, Universidad Católica del Norte, Center for Marine Research, Coquimbo, Chile.
Current main area of research
Design, construction and evaluation of vaccines to elicit protective host immune responses in agriculturally important aquatic animals, mechanisms of bacterial pathogenesis, bacterial genetics and host immune responses to infectious diseases and vaccines. Bacteriophage biology and food safety.
Peer-review Publications (*corresponding author)
1. Schoffer, JT., Valderrama, K., M. Ayala and J. Santander*. 2016. Analysis of Endogenous Insertional Arrangement in the vapA Locus of Aeromonas salmonicida. Genetic Mobile Elements (in preparation).
2. Valderrama, K. and J. Santander*. 2016. Phenotype and Virulence of Aeromonas salmonicida cyclic adenosine 3’,5’-monophosphate receptor protein (Crp) Mutants in Rainbow Trout (Oncorhynchus mykiss). J Fish Diseases (in preparation).
3. Segovia, C., K. Valderrama, D. Valenzuela-Miranda, C. Gallardo, J. Santander*. 2016. Gene Expression Under Iron Limitation in Piscirickettsia salmonis. Aquaculture (submitted)
4. Santander, J.*, T. Schoffer, C. Segovia, K. Valderrama, and P. Smith. 2016. Unique Constitutively Expressed Genes of Piscirickettsia salmonis. ABM Express (submitted).
5. Santander, J*, Vasquez, J.I., C. Segovia, G. Turra, K. Hober, and J. Robeson. 2016. Comparative Genomics of Salmonella enterica Serovar Enteritidis Bacteriophages fSE1C and fSE4S Isolated from Food Matrices in Chile. Standards in Genomic Sciences (submitted)
6. Saravia, M., C. Segovia, K. Valderrama, J. Santander*. 2016. Colibacillosis in a New Zealand White Rabbit (Oryctolagus cuniculus). J. Infection in Developing Countries (submitted)
7. Almarza, O., K. Valderrama, M. Ayala, C. Segovia and J. Santander*. 2016. A Functional Ferric Uptake Regulator (Fur) in the Fish Pathogen Piscirickettsia salmonis. Int. Microbiology (in press).
8. Rojas, R., J. Romero*, C. Miranda, J. Santander. 2016. First report of Vibrio tubiashii associated to larval massive mortality a commercial in hatchery of scallop Argopecten purpuratus in Chile. Frontier in Microbiology 7:1473. doi:10.3389/fmicb.2016.01473
9. Retamales, J., I. Vasquez, L. Santos, C. Segovia, M. Ayala, R. Alvarado, P. Nuñez, J. Santander*. 2016. Complete Sequence of Biocontroling Bacteriophages of Xanthomonas arboricola pv juglandis. Genome Announcements 4(3):e00336-16
10. Martin, T., I. Diaz, J. Kilbourne, O. Almarza, C. Segovia, R. Curtiss III and J. Santander*. 2016. Influence of Lipopolysaccharide Outer-Core in The Intrinsic Resistance to Antimicrobial Peptides and Virulence in Edwardsiella ictaluri. Microbial Pathogenesis 93:204-212 doi:10.1016/j.micpath.2016.02.020
11. Segovia C., Vasquez I., Maracaja-Coutinho V., Robeson J. and Santander J.* 2015. Complete Genome Sequence of the Salmonella enterica Serovar Enteritidis f18SE Bacteriophage Isolated in Chile. Genome Announcements 3(5):e00600-15
12. Rojas R., Miranda CD, Romero J., Asenjo F., Katherine V., Segovia C., Ugalde JA* and Santander J*. 2015. Genome Sequence of Vibrio VPAP30 isolated from an episode of massive mortality of reared larvae of scallop Argopecten purpuratus. Genome Announcements 3(4): e00745-15
13. Santander, J.*, C. Otto, D. Lowry, C. Salas, M. Cuellar, M. Mellado and C. Echiburu-Chau. 2015. Antibacterial mechanism of 4-hydroxy-3-(3-methyl-2-butenyl)acetophenone isolated from Senecio graveolens. British Microbiology Research Journal. 5(2): 94-106
14. Dankel, D.*, K. Brenneman, A. Delgado, M. Fisher, K. Roland, J. Santander, Ch. Baek, J. Clark-Curtiss, R. Strand, R. Curtiss III. 2014. Making common sense of vaccines: An example of discussing the Recombinant Attenuated Salmonella Vaccine with the public. Nanoethics. 8(2): 179–185. DOI 10.1007/s11569-014-0198-6
15. Santander, J.*, J. Kilbourne, J. Y. Park, T. Martin, A. Loh, M. I. Diaz, C. Segovia, R. Rojas, D. DeNardo and R. Curtiss III. 2014. Inflammatory Effects of Edwardsiella ictaluri Lypopolysaccharide in Catfish Gut. Infection and Immunity 82(8): 3394-3404.
16. Rojas, R.*, C. Segovia, N. Trombert, J. Santander, P. Manque. 2014. The effect of tunicamycin on the glucose uptake, growth and cellular adhesion in the protozoan parasite Crithidia fasciculate. Current Microbiol. 10.1007/s00284-014-0620-x
17. Echiburu-Chau*, C., Alfaro S., Brown N., Salas C., Cuellar M., Santander J., Ogalde J. P. and Rothhammer F. 2014. Selective cytotoxicity elicited by phytochemical extract from Senecio graveolens (Asteraceae) on breast cancer cells enhanced by hypoxia. Int. J. Oncol. 44(4):1357-1364
18. Guan, L., J. Santander, M. Mellata, Y. Zhang, and R. Curtiss 3rd*. 2013. Identification of an Iron Acquisition Machinery of Flavobacterium columnare. Dis. Aqua. Org. 106: 129–138.
19. Santander, J.*, T. Martin, A. Loh, C. Pohlez, G. M. Gatlin III and R. Curtiss III. 2013. Mechanism of Intrinsic Resistance to Antimicrobial Peptides of Edwardsiella ictaluri and its Influence on Fish Virulence and Gut Inflammation. Microbiology. 159:1471-1486. doi:10.1099/mic.0.066639-0
20. León, M., J. Santander, R. Curtiss III, and J. Robeson*. 2013. Natural Lysogenization and Transduction in Salmonella enterica Serovar Choleraesuis by Bacteriophage P1. Research in Microbiology. 164(1):1-5
21. Santander, J.* 2012. Edwardsiellosis, an Emerging Enteric Zoonotic of Aquatic Animals. Immunity and Diseases. 1(1):1-2.
22. Santander, J.* G. Golden, Soo-Young Wanda, and R. Curtiss III. 2012. The Fur Regulated Iron Uptake System of Edwardsiella ictaluri and its Influence on Pathogenesis and Immunogenicity in the Catfish Host. Infection and Immunity 80(8): 2689-2703.
23. Xin, W., S. Y. Wanda, X. Zhang, P. Alamuri, J. Santander, G. Scarpellini, K. Ellis, and R. Curtiss III*. 2012. Developing new non-antibiotic dual-plasmids Salmonella delivery system for carrying multiple pneumococcal antigens enhances protection against S. pneumoniae. Infection and Immunity. 80(10): 3621-3633.
24. Mellata, M.*, J. Maddux, T. Nam, N. Thomson, H. Hauser, M. Stevens, S. Mukhopadhyay, S. Sarker, A. Crabbé, C. Nickerson, J. Santander, R. Curtiss III. 2012. New Insights into the Fitness-associated Mechanisms of ExPECs Revealed by the Genotypic and Phenotypic Characterization of Large Plasmids of APEC χ7122 (O78:K80:H9). PloSOne. 7(1):e29481.
25. Santander, J, A. Mitra, and R. Curtiss III*. 2011. Phenotype, Virulence, and Immunogenicity of Edwardsiella ictaluri Cyclic Adenosine 3’,5’-Monophosphate Receptor Protein (Crp) Mutants in Catfish Host. Fish and Shellfish Immunology. 31:1142-1153.
26. Santander, J., W. Xin, Z. Yang, and R. Curtiss III*. 2010. The aspartate-semialdehyde dehydrogenase of Edwardsiella ictaluri and its use as balanced-lethal system in fish vaccinology. PloS One. 5(12):e15944.
27. Shi, H.Y, J. Santander, K. Brenneman, S. Y. Wanda, S. Wang, P. Senechal, W. Sun, K. Roland, and R. Curtiss III*. 2010. Live Recombinant Salmonella enterica Serovar Typhi Vaccines Constructed to Investigate the Role of rpoS in Eliciting Immunity to a Heterologous Antigen. PloSOne. 5: e11142
28. Santander, J., and R. Curtiss III*. 2010. Salmonella enterica Serovars Typhi and Paratyphi A are avirulent in newborn and infant mice even when expressing virulence plasmid genes of S. Typhimurium. J. Infection in Developing Countries. 4(11):723-731. doi:10.3855/jidc.1218
29. Santander, J., K. L. Roland and R. Curtiss III*. 2008. Regulation of Vi antigen in Salmonella enterica serovar Typhi. J. Infection in Developing Countries. Review article. 2(6):412-420.
30. Borie, C., P. Zurita, M. L. Sánchez, V. Rojas, J. Santander, and J. Robeson*. 2008. Prevention of Salmonella enterica subspecie enterica serotype Enteritidis (Salmonella Enteritidis) infection in chickens using a bacteriophage. Arch. Med. Vet. 40:197-201.
31. Santander, J. and J. Robeson*. 2007. Phage-Resistance in Salmonella enterica Serovar Enteritidis and Pathogenicity in Caenorhabditis elegans is Mediate by the Lipopolysaccharide. Electronic Journal of Biotechnology. Vol. 10, No. 4, Issue of October 15
32. Santander, J.. S. Wanda, C. A. Nickerson, and R. Curtiss III*. 2007. Role of RpoS in fine-tuning the synthesis of Vi capsular polysaccharide in Salmonella enterica serotype Typhi. Infection and Immunity. 75(3):1382-1392.
33. Santander, J. and J. Robeson*. 2004. Phage prophylaxis against Salmonella enteritidis using Caenorhabditis elegans as an assay system. Electronic Journal of Biotechnology. 7(2):11-14. Issue of August 15.
34. Santander, J., J. C. Espinoza, Mª S. Campano and J. Robeson*. 2003. Infection of Caenorhabditis elegans by Salmonella Typhi Ty2. Electronic Journal of Biotechnology. 6(2):148-152. Issue of August 15.
35. Santander, J. and J. Robeson*. 2002. Isolation and characterization of bacteriophages active against Salmonella enteritidis and their assay on Salmonella pullorum. Acta Microbiológica. 8(1):17-22.
Non-Peer Review Publications
1. Santander, J.*, O. Almarza and K. Valderrama. 2014. Bacterial genetic engineering applied to vaccine development. Different Vision Aquaculture Magazine.
2. Thompson B. 2013. E. ictaluri vs the catfish immune system: water warfare. Microbe Post a blog from the Microbiology Society. https://microbepost.org/2013/07/23/e-ictaluri-vs-the-catfish-immune-system-water-warfare/
3. Santander, J.* 2012. Developing live attenuated vaccines by deleting genes for iron metabolism in pathogenic bacteria. World Aquaculture Magazine. 43(3): 28-31.
ABSTRACTS, Conference And seminars
1. Retamales J., Santander J., Alvarado R., González P., Joublan J.P. Núñez P. 2016. Biocontrol of Xanthomonas arboricola pv. juglandis using bacteriophages: a sustainable alternative against bacterial walnut blight. III Chilean Symposium of Biological Control, Chillan, Chile
2. Santander, J*, K. Valderrama, S. Segovia, T. Shoeffer 2016. Unique Constitutively Expressed Genes of Piscirickettsia salmonis as Detection tool. Aquaculture, Edinburg, Scotland (oral presentation).
3. Segovia, C., J. Santander*. 2016. Non-coding RNA in Piscirickettsia salmonis under iron limited conditions. Aquaculture, Edinburgh, Scotland (poster presentation).
4. Santander, J*. 2016. Technological tools for the aquaculture. SalmoFood workshop, March 18, Puerto Montt, Chile.
5. Santander, J*., R. Rojas, J. Romero, L. Mercado and S. Rodriguez. 2015. Mini-symposia “Microbiology in the Sustainable Aquaculture”. Chilean Congress for Microbiology. La Serena, Chile. Oral presentation. (chair)
6. Segovia, C., V. Maracaja-Coutinho and J. Santander*. 2015. Differential expression of ncRNA of Piscirickettsia salmonis in limitation iron conditions. XXXVII Chilean Congress for Microbiology. La Serena, Chile. (poster presentation).
7. Vásquez J.I. and J. Santander*. 2015. Evaluation of Lipid A Modifications Toxicity in Zebra Fish Embryons (Danio rerio). XXXVII Chilean Congress for Microbiology. La Serena, Chile. (poster presentation) (Undergraduate Award)
8. Santander J.*, C. Segovia, O. Almarza, K. Valderrama, D. Valenzuela-Miranda, C. Gallardo. 2015. Gene Expression Under Iron Deprivation in Piscirickettsia salmonis. XXXVII Chilean Congress for Microbiology. La Serena, Chile. (poster presentation).
9. Almarza, O. and J. Santander*. 2015. A Functional Ferric Uptake Regulator (Fur) in the Fish Pathogen Piscirickettsia salmonis. XXXVII Chilean Congress for Microbiology. La Serena, Chile. (poster presentation).
10. Schoffer, JT, M. Ayala, K. Valderrama, O. Almarza and J. Santander*. 2015. Analysis of Endogenous Recombination of vapA locus in Aeromonas salmonicida After Thermal Shock. XXXVII Chilean Congress for Microbiology. La Serena, Chile. (poster presentation).
11. Valenzuela-Miranda D., C. Segovia, K. Valderrama, O. Almarza, C. Gallardo, J. Santander*. 2015. Molecular mechanisms orchestrating iron uptake in Piscirickettsia salmonis. Aquaculture, Montpellier, France (poster presentation).
12. Almarza, O., J. Santander*. 2015. The fur gene of Piscirickettsia salmonis. Aquaculture, Montpellier, France (oral presentation).
13. Segovia,C., M. Ayala, K. Valderrama, T. Schoffer, V. Maracaja-Coutinho, J. Santander*. 2015. Aeromonas salmonicida Transcriptomics Hunting for Loss of Virulence Mediated Thermal Shock. Aquaculture, Montpellier, France (poster presentation).
14. Valderrama, K., O. Almarza, J. Santander*. 2015. Aeromonas salmonicida cyclic adenosine 3’,5’-monophosphate receptor protein (Crp) mutants and its virulence in rainbow trout (Oncorhynchus mykiss). Aquaculture, Montpellier, France (poster presentation).
15. Martin, T., I. Diaz, J. Kilbourne, O. Almarza, C. Segovia, R. Curtiss III, J. Santander*. 2015. Influence of Glucuronic acid Transferase on Intrinsic Resistance to Antimicrobial Peptides and Virulence of Edwardsiella ictaluri. Aquaculture, Montpellier, France (oral presentation).
16. Segovia, C., K. Valderrama, V. Maracaja-Coutinho and J. Santander*. 2015. Non-coding RNA of Piscirickettsia salmonis in absence of iron. XXXV Chilean Congress for Marine Sciences, Coquimbo, Chile (oral presentation).
17. Valderrama, K., O. Almarza, T. Schoffer, J. Santander*. 2015. Phenotype of Aeromonas salmonicida Dcrp and DphoP mutant strains. XXXV Chilean Congress for Marine Sciences, Coquimbo, Chile (poster presentation).
18. López, N., A. Booker, M. Simirgiotis, G. León, S. Alfaro-Lira, C. Salas, T. Schoffer, M. Ayala, J. Santander, M. Heinrich and C. Echiburú-Chau*. 2015. Metabolomic variation in S. graveolens (Asteraceae) in altitudinal populations. 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research. Budapest, Hungary (poster presentation).
19. Santander, J. 2015. Bacterial Fish Pathogenesis and Vector Vaccine Delivery for Teleost. INCAR Seminar series, University of Concepcion, Chile (invited speaker).
20. Santander, J. 2015. Bacterial Pathogenesis and Live Recombinant Attenuated Vaccines, from mammals to fish. Research Seminar, Department of Ocean Sciences, Memorial University of Newfoundland, St John’s, Canada (invited speaker).