Human E2F Target Genes- Primer Sequences
fos L agg aac tgc gaa atg ctc ac
fos R gaa gca gag ctg ggt agg ag
Annealing temp 60C, prod size 302 bp
junB L tat aaa ggc gtg tgg ctc ag
junB R ggg ctg ttc cat ttt agt gc
Annealing temp 60C, prod size 341 bp
TGF-b L gcc cca gag tct gag acg ag
TGF-b R cga ggt ctg ggg aaa agt ct
Annealing temp 60C, prod size 333 bp
ARF L tgt cag gtg acg gat gta gc
ARF R caa gat ctc gga acg gct ct
Annealing temp 60C, prod size 262 bp
B-myb L ggt gtt cgc tat gtg gga ta
B-myb R ctc ctc gct cgc agg aac tg
Annealing temp 60C, prod size 311 bp
MCM5 L tcc ttc cca gcc aga agt tt
MCM5 R tcc cac tag cct cac ctc tg
Annealing temp 60C, prod size 300 bp
RNAP L aga ggc ggt ggg att aaa ga
RNAP R tcc tga acg gca gag gtt ac
Annealing temp 60C, prod size 303 bp
E2F2 L ggc tcg ctc tct agt cct tg
E2F2 R gtc tcg ccg gct gct aac ta
Annealing temp 60C, prod size 283 bp
Mcm4 L cca cca cct ccc gtc ctt aa
Mcm4 R aat cac agc ggc gct cgt ac
Annealing temp 60C, prod size 350 bp
H2A 5’ tga gga caa gct ggg tta tgc ccc ta
H2A 3’ gca ctc gtc cta cag gaa att gga g
ORC1 5’ cag ctt agg cga cca tct tga agc c
ORC1 3’ cta gcc gca agc cct ccg ttt cct a
MCM6 5’ act aga cag cag aag cgg ctt act c
MCM6 3’ tcg acc gcc aca agt cgc ttt ttt c
DNAPola 5’ ggg aaa atg ggc tct gag aac cag a
DNAPola 3’ tat ctc aca gtc gtc gcc gtg cac a
CycB 5’ ttt gac act tct gag act gtg gcc g
CycB 3’ caa gga cct aca acc agc aga aac c
Cdc6 5’ atg tag att ccc tcc ccc gtt cag t
Cdc6 3’ tcc tct tct ttc cac ctc ctc agt g
Mcm5 5’ agg ctg agg cgg aag gat tgc ttg a
Mcm5 3” ttt ccg cac ttc aca aac cag ggg a
RanBP L gtg ggg cgg agg gaa ga
RanBP R aaa ctg agg gtc atg gtt gg
Product Size: 217
Smad6 L gcg agg tcc ctc atg ttg tt
Smad6 R gtt ggg gac cac acg act tc
Product Size: 249
XRCC2 L caa gaa aag cac cag ctg tc
XRCC2 R gcc cta tgg aag gca cta ca
Product Size: 302
vh1 L gcc tcc gtt cac aca gcc ta
vh1 R tct tca ggg ccg tac tct tc
Product Size: 289
p53 L cac caa ttc tgc cct cac ag
p53 R tgg acg gtg gct cta gac tt
Product Size: 286
Nuc L ggg tct ctg agg aac tcc aa
Nuc R cca gta acg cct gtg gaa ag
Product Size: 367
DHFR+446 cta caa gtt aga gaa aca gcg tta ctc gaa
DHFR-17 ttc tgc tgt aac gcg cgg gct cgg a
cdc2-695 gtg cta gct cga gat ctt cc
cdc2-245 agc tac tgt tcg ctc cgt tc
Product Size: 400