Course Syllabus and Lesson Plan

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Course Syllabus and Lesson Plan

SCID 141

Living Processes: From Molecules to Cells

(Chemical processes in living system)

Academic Year 2012-2013: 12 November 2012 – 18 Febuary 2013

Course Title: Living Processes, From Molecules to Cells

Course Code: SCID 141

Total Credit: 3(3-0)

Pre-Requisite: SCBI 101, SCBI 102, SCBI 103, SCBI 104

Duration: Second semester

Course Homepage:

http://www.sc.mahidol.ac.th/scbc/webboard/index.php

Course Description:

Structures and functions of biomolecules, protein folding, protein functions, bio-catalysis, enzyme kinetics, citric acid cycle, electron transport and oxidative phosphorylation, anabolism and catabolism of biomolecules in normal and some important pathological stages, regulation of metabolic pathways, flow of genetic information, gene regulation, and molecular techniques with medical applications.

การศึกษาคุณสมบัติทางเคมีและชีววิทยาของชีวโมเลกุลในเซลล์สิ่งมีชีวิต ความสัมพันธ์ที่เกี่ยวข้องกับโครงสร้างและหน้าที่ ความสำคัญของการควบคุมขบวนการเมตาบอลิสมในสิ่งมีชีวิต พลังงานที่ใช้ในการสร้างและสลายทั้งโมเลกุลเล็กและโมเลกุลใหญ่ที่ทำหน้าที่อยู่ภายในเซลล์ปกติและสภาวะการเกิดพยาธิของโรค ความรู้พื้นฐานเกี่ยวกับการจัดเรียงของยีโนม รหัสพันธุกรรมและการถ่ายทอด รวมทั้งเทคนิคทางอณูชีววิทยาที่เกี่ยวข้องกับการประยุกต์ทางการแพทย์

Course Objectives:

At the end of this course, the students will be able to

1. explain chemical processes occurred in living system, structure and functional relationship of biomolecules

2. point out the regulation of metabolic pathways governing the life of a cell

3. know and understand energy metabolism, biosynthesis and degradation in both normal and some important pathological stages

4.  emphasize fundamental of genome organization, flow of genetic information and gene regulation

5.  learn the principle of molecular techniques relevant to medical applications.

Course Outlines:

See schedule on page

Teaching Methods

Lecture, Group study, Tutorial and assignment

Teaching Media

•  Text book Lehninger, Principle of Biochemistry, 4th or 5th edition,

•  CAI, VDO, PowerPoint and/or transparency presentation, can be self-study via web site: http://www.sc.mahidol.ac.th/scbc/webboard/index.php

Evaluation of Student Achievement

Grading system from midterm and final examination

•  Midterm written examination (MCQ) 30%

•  Final written examination (MCQ) 70%

Total 100%

Students who get a total grade more than 50% are considered successful for this course.

Course Evaluation

Questionnaire about contents, teaching processes, examinations and instructor performance.

Required Textbook

•  Lehninger, L. A., Nelson, D. L., and Cox, M. M., Principles of Biochemistry, 4th or 5th edition, Worth, New York

Other References

1.  Voet, D., and Voet, J. G., Biochemistry, John Wiley & Sons, New York, 1990.

2.  John, W. Baynes and Marek H. Dominiczak, Medical Biochemistry, 2nd edition, Elsevier Mosby, 2005

3.  Other equivalent biochemistry textbooks.

Instructors

Lecturer from Department of Biochemistry, Pharmacology, Physiology and Clinician and guest lecturers from outside Faculty of Science

Course Coordinators

Asist. Prof. Dr. Jamorn Somana

Department of Biochemistry, Faculty of Science, Mahidol University

Tel: 0-2201-5601 and 0-2201-5468

Course Syllabus and Lesson Plan

SCID 142

Laboratory in Living Processes

Academic Year 2012-2013: 23 November 2012 – 11 Febuary 2013

Course Title: Laboratory in Living Processes

Course Code: SCID 142

Total Credit: 1(0-3)

Pre-Requisite: SCBI 101, SCBI 102, SCBI 103, SCBI 104, SCID141

Duration: Second semester

Course Homepage:

http://www.sc.mahidol.ac.th/scbc/webboard/index.php

Course Description:

Determination of LDH enzyme activity by spectrophotometric method, Determination of liver enzyme activities, DNA isolation and PCR amplification followed with analysis of DNA by gel electrophoresis technique, structures and functions of biomolecules, protein folding, protein functions, bio-catalysis, enzyme kinetics, TCA cycle, electron transport and oxidative phosphorylation, anabolism and catabolism of biomolecules in normal and pathological stages, regulation of metabolic pathways, flow of genetic information, gene regulation, and molecular techniques with medical applications. Starvation

การวัดการทำงานของเอนไซม์ LDH โดยวิธีการดูดกลืนแสง การตรวจวัดเอนไซม์ในตับ การแยกดีเอ็นเอและปฎิกริยาพีซีอาร์พร้อมการวิเคราะห์โดยกระแสไฟฟ้า โครงสร้างและหน้าที่ของชีวโมเลกุล การม้วนตัวของโปรตีน โครงสร้างและหน้าที่ของโปรตีน ตัวเร่งชีวภาพ จนลศาสตร์ของเอนไซม์ วัฏจักรกรดซิตริก การถ่ายทอดอีเลคตรอน การสร้างเอทีพีในไมโตคอนเดรีย การสร้างและการสลายชีวโมเลกุลต่างๆในภาวะปกติและภาวะการเกิดโรค การควบคุมขบวนการเมตาบอลิสมในสิ่งมีชีวิต รหัสพันธุกรรมและการถ่ายทอด การจัดเรียงและการแสดงออกของยีโนม เทคนิคทางอณูชีววิทยาที่เกี่ยวข้องกับการประยุกต์ทางการแพทย์ การอดอาหาร

Course Objectives:

At the end of this course, the students will be able to

1. have a laboratories skill on basic techniques in structure and functional relationship of biomolecules

2. gain the knowledge and understand the way to analysis the experimental data

3. work together or have group activities relevant to chemical processes occurred in living system and medical applications

4. acquire necessary knowledge through current available resources and analyze critical problems and data obtained.

Course Outlines:

See schedule on page

Teaching Methods:

Laboratory, Group study, Discussion and assignment

1. Laboratories: Compulsory

There are 3 laboratory exercises designed to broaden the students’ skill and knowledge. These are 1) determination of LDH enzyme activity and studying LDH enzyme kinetics by spectrophotometric method 2) determination of liver enzyme activities 3) DNA isolation, PCR amplification and analysis of DNA by gel electrophoresis technique.

2. Small Group or Conferences: Compulsory

There are 3 small group discussions. The sessions involve discussion on problem sets related to the topics SCID 141 and some clinical cases. Student presentation and discussion will be focused and all sessions will have either pre- or post discussion quizzes.

3. Problem-Based Learning: Compulsory

Problem-based scenario will be given to initiate group activities in the discussion and self-study sessions to find out a possible explanation for the problem. Developments of conceptual thinking skill and integration of information are the main objective.

4. Team-Based Learning: Compulsory

There is 1 team-based learning topic given to initiate group activities in the learning processes. Students have to study the assignment topics before a class. Questions involved in the topics will be asked and the students have to find out the answers individually then try to find out within their group. Developments of conceptual thinking by him- or her-self and by group with integration of information are the major objective of this learning.

5. Tutorials: Not Compulsory

There are 3 tutorial periods to cover the basic concepts of the course. Tutorial period is provided to help students better understand the topics and/or clarify some points after their self- or group-study. Therefore, students are expected to go through the topics and prepare questions prior to each tutorial discussion. Questions for each tutorial session can be posted at the web-board (see course homepage) or can be submitted directly to the lecturer(s). All tutorials will be held at lecture hall.

6. CAI (CD and VDO): Compulsory

There are 1 CAI media available in this course, i.e. structure folding and build block, high throughput automated system, and molecular diagnosis, PCR and sequencing technique. These learning materials will be installed in the computer system and the students can access and study during free time. One CAI-media on PCR and DNA sequencing techniques is used in a class discussion.

Teaching Media:

•  Laboratory manual of the course

•  Text book Lehninger, Principle of Biochemistry, 4th or 5th edition,

•  CAI, VDO, PowerPoint and transparency presentations, can be self study via web site: http://www.sc.mahidol.ac.th/scbc/webboard/index.php

Evaluation of student achievement:

Grading system from written examination and work assignments or performance in the activities

Process 85%

Small group (5% x3), pre- or post-test (5%x3) 30%

PBL (10% x 2) 20%

Laboratories (5% x 4) 20%

CAI (5%) 5%

TBL iRad 5% tRad 2.5% gRad 2.5% 10%

Knowledge 15%

From written examination (Midterm and Final)

Out of the total 100%, students who get a grade more than 60% are considered successful for this course

Course Evaluation:

Questionnaire about contents, teaching processes, examinations and Instructor performance.

Required Textbook

•  Lehninger, L. A., Nelson, D. L., and Cox, M. M., Principles of Biochemistry, 4th or 5th edition, Worth, New York

Other References:

1.  Voet, D., and Voet, J. G., Biochemistry, John Wiley & Sons, New York, 1990.

2.  John, W. Baynes and Marek H. Dominiczak, Medical Biochemistry, 2nd edition, Elsevier Mosby, 2005

3.  Other equivalent biochemistry textbooks.

Instructors:

Lecturer from Department of Biochemistry, Pharmacology, Physiology and Clinician from Ramathibodi and guest lecturers from outside Faculty of Science.

Course Coordinators:

Asist. Prof. Dr. Jamorn Somana

Department of Biochemistry, Faculty of Science, Mahidol University

Tel: 0-2201-5601 and 0-2201-5468

ตารางสอนรายวิชา SCID 141-142 ประจำภาคปลายปีการศึกษา 2555-2556

กลุ่มนักศึกษา RA, BM, PI

หมายเหตุ ห้องเรียนกลุ่มย่อย จันทร์, ศุกร์ SC1-139, SC1-141, SC1-220, SC1-221, SC1-321, SC1-322, SC2-133, SC2-134, SC2-136, SC2-139, SC2-140, SC2-142, SC2-222, SC2-322

LESSON PLAN

Lecture 1: Structure and Function of Biomolecules

Instructor: Kittisak, Biochemistry Faculty of Science