Conservation Genetics – Electronic Supplementary Material

Translocation retains genetic diversity of a threatened endemic reptile in Mauritius

Sozos Michaelides1*, Nik Cole2, 3, Stephan M. Funk4,5

1 Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS, UK

2 Durrell Wildlife Conservation Trust, Les Augrès Manor, Trinity, Jersey JE3 5BP, Channel Islands

3 Mauritian Wildlife Foundation, Grannum Road, Vacoas, Mauritius

4 Nature Heritage, St. Lawrence, Jersey, Channel Islands

5 Universidad Católica de Temuco, Núcleo de Estudios Ambientales, Temuco, Chile

* Corresponding author: phone: +441865271173, email:

Table S3 Details on the primers set, the M13 tail tag and the fluorescent dye used

Locus / Forward Primer / Reverse Primer / M13 tail a / Dye b
GB01 / GGTGTGACCAAAACCCTAAGAACC / TGGCAAAAAAGCAATGGAGC / R / NED
GB02 / TGACCCCTGTGGTTTTTTGAC / AAAGGAGTAACGGAAAGTTGCC / F / 6-FAM
GB03 / CATCAATGTCAACAAGGAACTGGC / TGCTATCTACCAAAGTGGCTGCTG / F / PET
GB04 / GCCCAAGCACCAGAATAGTGC / CCCTCAAGCCTCAAAACCACAG / F / 6-FAM
GB05 / TCGTTTGCTTCCCTTCCCC / TTACGCACATACCAAGAAATGGC / F / VIC
GB06 / ATTAGGGCTCTGTTACAAGCAG / CCACAGGTACATACTAACTAGGACC / F / VIC
GB07 / TGAGTTGCATGGCTTTGG / GATCGCCCAAGTACAGCAT / F / 6-FAM
GB08 / TCACCTGTCCCTTTTCTGTGG / ATACTATCCCCCATCATTGGC / R / PET
GB09 / CAGTTCTCTGAGGGAAGTTTTGC / GCTCCGTGAATGTCTCTTGG / R / NED
GB10 / GCCCAGAGACTCAATCTAGCC / TCGGACATGACTTAGCGACTG / F / 6-FAM
GB11 / GATCCTCCTTTTTTCACACACT / CCAAGAGTTTTTGCCTTTCC / R / PET
GB12 / GGTGGTTACAGGCAGGTTTTAGG / TTCCCATCAAATGGTGGTGC / R / VIC
GB13 / CTGAACTTGCAGCCCCAGTG / CATCAGCAGTCAAGACCAAAACC / F / PET
GB14 / CGGGTTCAAATGGAGAATAGTTC / GATCCAGCAGGGCTTTTCTTAC / R / NED
GB15 / TGCTGCTGTTGCTGCCTTG / TTTGGAGTACCTGCCTGTAAGCC / F / NED

a Indicates where the M13 tail is attached (F- forward primer, R- reverse primer)

b Each universal M13 primer is fluorescently labelled with one fluorescent dye