6. BRIEF RESUME OF THE INTENDED WORK:
6.1 NEED FOR THE STUDY:
Reproduction is an important phenomenon of the living organisms and is vital process that enables a species to represent itself in the species.
The population in India has been growing rapidly there is increased demand on food diseases, destructive activities, illiteracy, air and water pollution and land effluents waste products.
Today there is need to limit the family size at a personal and to control the population at National level. Because, increased cost of living scarcity of accommodation, a desire for better education to children in the present competitive world and on overall desire for an improved standard of living.
Ultimately for birth control there is need of contraceptives. In1956 Rock, Garcia, Pincus from United States of America first invented the oral contraceptive drug. Today’s almost all contraceptives are expensive having side effects and complications. Contraceptives agents like Mala-D, Mala-N, Ovral-L are combination of estrogen and progesterogen have lot of side effects.
Due to these hazardable effects women are worried about these contraceptives. They need effective, safe, economic, reliable, acceptable contraceptive with little skill to use.
Ancient Ayurvedic scholars have practiced with different safe contraceptives. The contraceptive has been mentioned in the single drug and compound formulation for example single drugs are Dattura,Chitraka,Patha etc.Athenticated Patha has described as the best contraceptive in Bhaishajya Ratnavali. Hence this project will be undertaken for study.
6.2 REVIEW OF LITERATURE:
Patha leaves are use in Yonivyapad Vyadhis1.
It has been explained that in Asti Vikaras.2
It has been explained in Pippalyadi varga.3
Leaf juice used in Krimi Roga.4, 5
Patha leaves extract helpful in Stanyashodhaka and in Contraceptive activity.6, 7, 8
PREVIOUS WORK DONE:
1. Dr. Pati H.K. : Anti-fertility effect of Jayanti, Banaras Hindu
University, Varanasi-1976.
2. Dr. Sinna(Km) Mukta : Oral contraceptive properties of certain indigenous
drugs in female rats; an experimental study, Banaras
Hindu University, Varanasi -1978.
3.Dr. Reddy S.N. : Anti-fertility effect of same indigenous drugs in
combinations, Kerala University,
Thiruvanantapuram- 1988
4 . Dr. Ramesha. M. : Evaluation of certain indigenous drugs for their
efficacy as oral contraceptives with trials on
experimental animals, L-1735, Gujarat
University, Jamanagar-1989
5. Dr. Upadyaya Manoja : Evaluation of certain herbal contraceptive
described in Ayurvedic classics on modern
parameters. University of Lucknow - 2001
6.3 OBJECTIVES OF STUDY:
1)Morphological and Microscopical study of Patha.
2)Extraction and Phytochemical investigation of Patha.
3)Fractionation by UV Spectroscope.
4)To assess the contraceptive effect of Patha.
7. MATERIAL AND METHODS :
7.1 SOURCE OF DATA:
Material and Methodsfor present study includes.
- Literary Sources of data :
Literary Sources required for the present study will be referred through classical literatures like Priya Niganthu, Nighanthu Adarsha, Bhavaprakasha, Bhaishajya Ratnavali, etc and compilation of modern texts, journals, different websites etc.
- Experimental Sources of data :
The present study will be aimed at phytochemical investigation and experimental work in laboratory. The experimental study will be carried on healthy adult female Albino Rats.
7.2 METHODS OF COLLECTION OF DATA:
(INCLUDING SAMPLING PROCEDURE IF ANY)
The leaves of Patha will be collected from wild source and will be identified thoroughly by morphological and microscopical studies.
For the present study dried leaves of Patha will be extracted with help of soxhlate apparatus. The crude extract of Patha will be subjected to contraceptive and anti-implantation activity.Based on the contraceptive and anti-implantation activity on Fimale Albino Rats and hostological observation, inference will be made.
I. Grouping: All the healthy adult Albino Ratsof female sex, weighing from 110gms- 180gms will be subjected to detailed examination and will be grouped into three groups of 6 Rats each for contraceptive and anti-implantation activity.
a. Trial group treated with test drug.
b.Standard group treated with standard drug.
c.Control group treated with vehicle only.
II. Monitoring:
The anti-implantation activity is determined by the method of Khanna and Choudhary (1968). Vaginal smear will be examined for proestrous cycle and after it will be kept for mating with ratios of (3 female, 1 male Albinorats). Different does of the crude extract of Patha, Standard and vehicle drugs will be administered orally with the help of intra – gastric catheter. On the tenth day rats will be laprotomised under light ether anesthesia to know the cornification of vagina and implantation sites in the horns of the uteri to detect the antifertility activity.
7.3 DOES THE STUDY REQUIRE ANY INVESTIGATIONS OR
INTERVENTIONS TO BE CONDUCTED ON PATIENTS OR OTHER HUMAN OR ANIMALS? IF SO PLEASE DESCRBE BRIEFLY
NO
7.4 HAS ETHICAL CLEARANCE BEEN OBTAINED FROM YOUR
INSTITUTION IN CASE OF 7.3?
Yes, Ethical committee has recommended this project and advised to participating in experimental analysis.
8. LIST OF REFERENCE:
1)Sharma. P. V.; Priya Nighantu, Hindi commentary 1st ed, Chaukambha Surabharati Prakashana; Varanasi: 1983, 61pp.
2)Tripathi Indradeva; Raja Nighantu, 2nd ed, Krishnadas Academy, Varanasi, 1998, 156pp.
3)Tripathi Indradeva; Raja Nighantu,2nd ed, Krishnadas Academy, Varanasi, 1998, 156pp.
4)Sharma. P. V.; Kaiyadeva Nighantu, Hindi commentary 1st ed, Chaukambha Orientalia, Varanasi, 1979, 125pp.
5)Sharma. P. V.; Dhanvantari Nighantu, Hindi commentary 2nd ed, Chaukambha Orientalia, Varanasi, 1998, 28pp.
6)Misra Brahmasankara; Bhavaprakasha Nighantu, Hindi commentary, vol-I, 9thed, Chaukambha Sanskrita Sansthan, Varanasi, 1999,364 pp.
7)Dasji Govinda; Bhaishajya Ratnavali, vol-III, 1st ed, Chaukambha Sanskrita Bhavan; Varanasi: 2006, 365 pp
8) Pulok Mukharjee; Quality Control of Syndicate Binders, New Delhi:2002, 578pp