Table S1. Oligonucleotide primers used to detect squash leaf curl virus by polymerase chain reaction.

Primer Name / Sequence(5`- 3`) / Target / Fragment length
SLCVA2295F 1 / CAG ATA ATT GAA TGA GGC AG' / DNA-A / 1351bp
Xho-SLCV-R (1004-987) 1 / TGT ACT CGA GAA TCA TGA AAT AAA ATT C
SLCVA2314R1 / CTG CCTCAT TCA ATT ATC TG / DNA-A / 1432bp
Xho-SLCVA-F (988-1012) 1 / CAT GAT TCT GAG TAC ATA ATT TAC
SLCV-DNA-B1-F / GTGGTTATGCAAGGCGTCGACCCAAC / DNA-B / 1316bp
SLCV-DNA-B1-R / GCAAACTGAAGCTATCGTCGGCGAAGC
SLCV-DNA-B2-F / GCTTCGCCGACGATAGCTTCAGTTTGC / DNA-B / 1644bp
SLCV-DNA-B2-R / GTTGGGTCGACGCCTTGCATAACCAC

1 Primers sequences were kindly provided by Dr. Moshe Lapidot (Agricultural Research Organization, Israel).

Table S2

Begomovirus taxa and the respective GenBank accession number for the DNA-A and DNA-B component, respectively, for each species or strain used in the phylogenetic analyses.

Begomovirus / Acronym / DNA-A / DNA-B
Squash leaf curl virus - Lebanon / SLCV-LB2 / HM368373 / HM368374
Squash leaf curl virus - Jordan / SLCV-JO-2 / JX131281 / JX131282
Squash leaf curl virus- Homra-Jordan / SLCV-Homra / JX444577 / JX444574
Squash leaf curl virus - Israel / SLCV-IL / HQ184436 / HQ184437
Squash leaf curl virus - Cairo / SLCV-EG / DQ285019 / DQ2850200
Squash leaf curl virus Arizona 10 / SLCV-AZ -Isolate 10 / DQ285016 / DQ285017
Squash leaf curl virus circular viral / SLCV-E / M38183 / M38182
Squash leaf curl virus –Malva-Jordan / SLCV-JO-Mal-DoI / EF532620 / EF532621
Squash leaf curl virus strain SLCV-WAZ / SLCV-WAZ / AF256203 / DQ285018
Squash yellow mild mottle virus isolate 98-631 / SYMMV-R-98-631 / AY064391 / AF440790
Squash mild leaf curl virus isolate 4 / SMLCV-Arizona / DQ285014 / DQ285015
Squash mild leaf curl virus-[Imperial Valley] SLCV-R / SMLCV-IV / AF421552 / NC_004646
Euphorbia mosaic virus [CU: Havana 27: 2007] / EuMV-CU-Havana / HQ896201 / HQ896202
Euphorbia mosaic virus isolate Eu4 / EuMV-Eu4 / JQ963887 / JQ963888
Euphorbia mosaic virus - A [Mexico:Yucatan:2004] / EuMV-pEuMV-YP / DQ318937 / DQ318938
Tomato mild yellow leaf curl Aragua virus-[Venezuela:10:2003] / TMYLCV- VE / AY927277 / EF547938
Euphorbia mosaic Venezuela virus isolate Aragua Eu1 / EuMV-Aragua EU1 / JN368145 / JN368146
Cucurbit leaf curl virus / CuLCV-Cal / AF224760 / AF224761
Cucurbit leaf crumple virus / CuLCrV-US-Cal-98 / NC_002984 / NC_002985
Euphorbia yellow mosaic virus-Goias [Brazil:DF:Taquara 8880:2009] / EuYMV-Goias / JF756673 / JF756678
Melon chlorotic leaf curl virus-[Guatemala] / MChLCV-Gua / AF325497 / AF325498
Bean calico mosaic virus / BCMV / AF110189 / AF110190
Rhynchosia golden mosaic Yucatan virus clone Desm-C6 / RhGMYV / GQ352453 / GQ352454
Rhynchosia mosaic Sinaloa virus / RhMSV / DQ406672 / DQ406673
Euphorbia yellow mosaic virus isolate BR:Car2:09 / EuYMV-BR:Car2:09 / JN419000 / JN419001
Cabbage leal curl virus isolate Jamaica clone pCRFA13 / CLCV-pCFRA13 / DQ178612 / DQ178613
Euphorbia mosaic virus isolate Brazil:Mato / EuMV-BR-Mato / FN435997 / FN435998
Pepper golden mosaic virus isolate Guanajuato1 / PepGMV-Gua1 / GU128148 / GU128147
Squash leaf curl virusisolateYL- Taiwan / SLCV-YL / EU479710 / EU479711
Watermelon chlorotic stunt virus Isolate Jordan / WmCSV-JO / JX131283 / JX131284
Watermelon chlorotic stunt virus isolate-Israel / WmCSV-IL / EF201809 / EF201810

SLCV-[PAL] DNA-A (KC441465), DNA-B (KC441466)