Suppl. Table 4 Individual scintigraphy results 6 and 20 hours after the first and the second bout of eccentric exercise.Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The accumulation of radioactivity was calculated as a ratio between a region of interest (ROI) in the exercised arm and radioactivity in the same ROI in the control arm, corrected for background radiation.

SUBJECT / Time after eccentric exercise
first bout / second bout
6h / 20h / 6h / 20h
CEL S05 (m) / 1.34 / 1.30 / 1.04 / 1.07
CEL S07 (f) / 1.35 / 1.58 / * / *
CEL S15 (m) / 1.11 / 1.17 / 1.37 / 1.36
PBO S04 (m) / 1.30 / 1.37 / * / *
PBO S12 (m) / 1.23 / 1.17 / * / *
PBO S28 (f) / 1.35 / 1.46 / 1.49 / 1.52
CEL S01(m) / 1.16 / 1.74 / 1.28 / 1.38
CEL S10 (m) / 1.23 / 1.34 / 1.12 / 1.06
PBO S02 (m)2 / 1.42 / 2.28 / 1.41 / 1.31
PBO S06 (f) / 1.92 / 3.40 / 1.45 / 2.20
PBO S08 (m) / 2.09 / 1.98 / * / *
PBO S14 (m)1 / 1.04 / 1.17 / * / *
PBO S16 (f) / 1.31 / 1.32 / * / *
PBO S18 (m) / * / * / * / *
PBO S25 (m) / 1.30 / 1.50 / * / *
PBO S27 (f) / 1.36 / 1.37 / 1.28 / 1.14
CEL S03 (m) / 1.25 / 1.79 / * / *
CEL S11 (f)2 / 2.20 / 3.86 / 1.78 / 2.56
CEL S17 (m) / 1.46 / 2.00 / 1.74 / 1.76
CEL S22 (m) / * / * / * / *
CEL S26 (f)2 / 2.14 / 3.27 / * / *
PBO S13 (m)1 / 1.48 / 2.36 / 2.12 / 2.10
PBO S29 (m) / 1.91 / 2.53 / 1.98 / 1.68

1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise.

21000% higher radioactivity compared same area in the contralateral arm was observed in small area of the mid part of m. biceps brachii at 20 h after the first exercise bout

*Subject didn’t participate to the measurements

f female, m male

Suppl. Table 5.Number of CD66b positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cell indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cell indicates deformation in the skeletal muscle fibre structure.

SUBJECT /

Time after the first bout of eccentric exercise

/ second bout
1h / 2d / 4d / 7d / 1h/2d
ex / cont / ex / cont / ex / cont / ex / cont / ex / cont
CEL S05 (m) / 0
(127) / 0
(96) / 0
(126) / 5
(147) § / 0
(142)a / 0
(86)a
CEL S07 (f) / 0
(354) / 0
(528) / 0
(187) / 0
(371) / 0
(184)a / 0
(194)a
CEL S15 (m) / * / 0
(261) / 0
(240) / 0
(256) / 0
(259)b / 0
(182)b
PBO S04 (m) / 0
(280) / 0
(121)
PBO S12 (m) / 0
(155) / 0
(277) / 0
(90) / * / 0
(161)a / 0
(74)a
PBO S28 (f) / 0
(255) / 0
(266) / 0
(147) / 0
(291) / 0
(181)b / 0
(275)b
CEL S01 (m) / 3
(106) # / 0
(232) / 2
(131) # / 0
(93) / 0
(154)b / 0
(159)b
CEL S10 (m) / 0
(110) / 0
(166) / 0
(247) / 0
(184) / 0
(248)a / 1
(125)a #
PBO S02 (m) / 0
(140) / 3
(146) § / 0
(56) / * / 0
(46)b / 0
(55)b
PBO S06 (f) / 2
(65) # / 0
(115) / 0
(345) / 4
(94) # / 1
(294)b # / 1
(238)b #
PBO S08 (m) / 0
(212) / 0
(194) / 0
(187) / 0
(168) / 0
(157)a / 2
(49)a #
PBO S14 (m)1 / 1
(238) # / 5
(255) § / 0
(185) / 0
(295) / 1
(335)b # / 0
(173)b
PBO S16 (f) / 1
(194) § / 0
(224) / 3
(135) § / 0
(205) / 0
(145)a / 0
(191)a
PBO S18 (m) / 0
(186) / 0
(178) / 30
(184) § / 20
(188) § / 0
(182)a / 0
(168)a
PBO S25 (m) / 0
(135) / 0
(219) / 2
(58) # / 0
(169) / 3
(106)b # / 0
(176)b
PBO S27 (f) / 0
(241) / * / 0
(195) / 0
(199) / 0
(235)b / 1
(207)b #
CEL S03 (m) / 0
(165) / 0
(54) / * / 0
(63)
CEL S11 (f) / 0
(346) / 0
(289) / 1
(434) § / 0
(369) / 0
(257)b / 0
(339)b
CEL S17(m) / 2
(187) # / 0
(98) / 1
(266) # / 0
(174) / 2
(258)a # / 2
(245)a #
CEL S22 (m) / 2
(100) § / 4
(100) #
CEL S26 (f) / 0
(295) / 1
(229 )# / 0
(148) / 0
(292) / 1
(207)b § / 1
(247)b §
PBO S13 (m)1 / 0
(227) / 0
(345) / 0
(205) / * / 0
(362)b / 0
(260)b
PBO S29 (m) / 4
(118) # / 1
(213) # / 0
(267) / 1
(204) # / 0
(257)a / 1
(222)a #

1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise.

a 1h and b 2 d after the second bout of exercise.

*Counting of CD66b positive cells was not possible due to bad quality of stained sections.

# CD66b positive cells were located in inside capillaries or vessels, or in blood clots

§ CD66b positive cells were located inthe endomysium or inside damaged fibres.

f female, m male

Suppl. Table 6Number of CD16 positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cells of the table indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cells of the table indicates deformation in the skeletal muscle fibre structure.

SUBJECT / Time after the first bout of eccentric exercise / second bout
1h / 2d / 4d / 7d / 1h/2d
ex / cont / ex / cont / ex / cont / ex / cont / ex / cont
CEL S05 (m) / 4
(139) / 2
(96) / 26
(115) / 317
(103) / 11
(106)a / 8
(84)a
CEL S07 (f) / 5
(254) / 2
(415) / 2
(136) / 23
(270) / 4
(108)a / 4
(121)a
CEL S15 (m) / * / 6
(205) / 6
(216) / 4
(221) / 37
(175)b / 15
(123)b
PBO S04 (m) / 18
(208) / 6
(84)
PBO S12 (m) / 3
(100) / 4
(257) / 8
(59) / * / 1
(114)a / 2
(54)a
PBO S28 (f) / 5
(244) / 5
(179) / 11
(107) / 17
(116) / 16
(124)b / 3
(214)b
CEL S01 (m) / 22
(59) / 15
(151) / 51
(98) / 57
(89) / 23
(86)b / 2
(119)b
CEL S10 (m) / 1
(110) / 1
(140) / 54
(175) / 4
(168) / 3
(233)a / 4
(123)a
PBO S02 (m) / 29
(125) / 4
(122) / 109
(35) / 7
(125) / 87
(23)b / 12
(41)b
PBO S06 (f) / 32
(38) / 1
(93) / 70
(266) / 17
(87) / 71
(282)b / 3
(203)b
PBO S08 (m) / 9
(136) / 10
(101) / 100
(128) / 118
(119) / 27
(101)a / 2
(49)a
PBO S14 (m)1 / 12
(129) / 77
(195) / 20
(104) / 3
(159) / 14
(242)b / 3
(166)b
PBO S16 (f) / 7
(137) / 7
(151) / 232
(92) / 2
(173) / 2
(129)a / 2
(134)a
PBO S18 (m) / 1
(160) / 4
(135) / 226
(84) / 239
(83) / 22
(68)a / 1
(79)a
PBO S25 (m) / 5
(56) / 7
(157) / 4
(113) / 1
(84) / 4
(55)b / 3
(64)b
PBO S27 (f) / 22
(194) / * / 626
(195) / 1
(179) / 33
(159)b / 11
(132)b
CEL S03 (m) / 23
(94) / 3
(58) / * / 7
(45)
CEL S11 (f) / 25
(260) / 2
(209) / 109
(356) / 16
(244) / 36
(197)b / 19
(151)b
CEL S17 (m) / 6
(122) / 0
(63) / 22
(179) / 2
(125) / 81
(158)a / 0
(168)a
CEL S22 (m) / 89
(204) / 2
(192)
CEL S26 (f) / 54
(227) / 4
(232) / 32
(183) / 25
(162) / 70
(97)b / 31
(177)b
PBO S13 (m)1 / 8
(227) / 1
(345) / 713
(205) / * / 72
(362)b / 4
(260)b
PBO S29 (m) / 0
(72) / 1
(145) / 1
(193) / 12
(94) / 96
(151)a / 0
(140)a

1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise.

a 1h and b 2 d after the second bout of exercise.

*Counting of CD16 positive cells was not possible due to bad quality of stained sections.

f female, m male

Suppl. Table 7Number of CD68 positive cells per 100 muscle fibres. Subjects were divided into three categories: mild (white background in the cell of subject’s label), moderate (light grey) and severe (dark grey) effect of eccentric exercise. The numbers in the brackets are the total number of muscle fibres from the area included for counting the stained cells. The black background in the small cells of the table indicates that one or more skeletal muscle fibres showed dystrophin negative staining in the section. Dark grey background in the small cells of the table indicates deformation in the skeletal muscle fibre structure.

SUBJECT / Time after the first bout of eccentric exercise / second bout
1h / 2d / 4d / 7d / 1h/2d
ex / cont / ex / cont / ex / cont / ex / cont / ex / cont
CEL S05 (m) / 21
(131) / 10
(84) / 19
(144) / 108
(138) / 14
(203)a / 31
(68)a
CEL S07 (f) / 7
(216) / 11
(551) / 17
(188) / 23
(349) / 15
(143)a / 17
(133)a
CEL S15 (m) / * / 9
(213) / 29
(115) / 15
(185) / 48
(145)b / 33
(153)b
PBO S04 (m) / 38
(203) / 19
(64)
PBO S12 (m) / 13
(116) / 20
(261) / 20
(59) / * / 31
(127)a / 28
(64)a
PBO S28 (f) / 23
(224) / 16
(189) / 16
(99) / 12
(186) / 46
(158)b / 11
(193)b
CEL S01(m) / 61
(66) / 28
(180) / 83
(90) / 70
(84) / 29
(106)b / 13
(92)b
CEL S10 (m) / 12
(118) / 12
(147) / 95
(212) / 5
(198) / 14
(233)a / 20
(116)a
PBO S02 (m) / 53
(138) / 32
(121) / 148
(27) / 49
(65) / 57
(28)b / 10
(58)b
PBO S06 (f) / 51
(53) / 22
(97) / 100
(105) / 20
(96) / 75
(251)b / 20
(167)b
PBO S08 (m) / 12
(130) / 23
(115) / 61
(150) / 83
(157) / 33
(153)a / 10
(78)a
PBO S14 (m)1 / 40
(233) / 49
(167) / 19
(111) / 21
(185) / 63
(284)b / 72
(152)b
PBO S16 (f) / 25
(62) / 19
(123) / 263
(72) / 18
(102) / 66
(102)a / 42
(130)a
PBO S18 (m) / 17
(147) / 11
(129) / 124
(119) / 142
(118) / 106
(107)a / 19
(119)a
PBO S25 (m) / 81
(96) / 24
(117) / 43
(111) / 34
(116) / * / 28
(92)b
PBO S27 (f) / 52
(157) / * / 968
(184) / 15
(144) / 45
(165)b / 21
(140b
CEL S03 (m) / 70
(74) / 45
(98) / * / 18
(40)
CEL S11 (f) / 26
(268) / 11
(337) / 73
(454) / 18
(245) / 50
(244)b / 35
(313)b
CEL S17 (m) / 38
(147) / 20
(88) / 32
(191) / 18
(170) / 75
(242)a / 10
(258)a
CEL S22 (m) / 204
(153) / 26
(120)
CEL S26 (f) / 51
(265) / 27
(247) / 54
(180) / 41
(148) / 66
(90)b / 67
(171)b
PBO S13 (m)1 / 49
(187) / 5
(153) / 932
(196) / 103
(87) / 156
(300)b / 43
(184)b
PBO S29 (m) / 24
(202) / 10
(206) / 16
(251) / 40
(167) / 155
(217)a / 9
(181)a

1 microdialysis in both exercised and non-exercised arm 2 h after the first bout of exercise.

a 1h and b 2 d after the second bout of exercise.

*Counting of CD68 positive cells was not possible due to bad quality of stained sections.

f female, m male