Code……. Course item: ………

1.  INFORMATION ABOUT THE COURSE

A.  Basic information

Name of course / Animal Research Models
Study level / Second degree
Unit running the study programme / Faculty of Animal Breeding and Biology, Department of Animal Biotechnology and Histology
Study programme / Biotechnology
Speciality / Biotechnology in Animal Production
Name of teacher (s) and his academic degree / Maria Siwek, PhD, Katarzyna Stadnicka, PhD, Anna Sławińska, PhD
Introductory courses / Biotechnology of Reproduction, Cell Engineering, Animal Embryology, Cell and Tissue Cultures
Prerequisites / Basic knowledge of molecular biology and animal breeding

B.  Semester/week schedule of classes

Semester / Lectures / Classes / Laboratories / Project / Seminars / Field exercises / ECTS
winter / 1 / 4

2.  EFFECTS OF EDUCATION (acc. to National Qualifications Framework)

Knowledge / Realized aim of course is to acquaint students with basic information about methodology of research, design of experiments with livestock animals, designing laboratory experiments.
Skills / On successful completion of the course student will be able to prepare an experimental design to verify scientific hypothesis, critically discuss results of the experiment, design practical experiment according to good laboratory practice.
Competences / On successful completion of the course student will be competent in application of in vitro and in vivo models in animal research

3.  TEACHING METHODS

Presentations, discussions, workshops

4.  METHODS OF EXAMINATION

Project presentation

5.  SCOPE

Lectures / Rationale for research using animal model; different examples of research on animal model in vivo and in vitro; design and methodology of research; statistical approach; Processing of the animal samples, including DNA, RNA and protein isolation; qualitative and quantitative evaluation of nucleic acids; preparation of the culture media; primary cell culture preparation; cell line passaging and cryopreservation;

6.  LITERATURE

Basic literature / Series of “Methods in Molecular Biology” Humana Press
Michael McPherson and Simon Moller, PCR. The basics. Tayor & Francis
R. Ian Freshney, Culture of Animal Cells: A manual of basic technique. John Wiley & sons
Supplementary literature / Scientific journals on animal science, biotechnology and molecular biology