Table S2: Genes in which transposon insertions were not obtained representing putative essential genes under the conditions tested1
Synonym / Gene / Total TAs2 / Informative TAs3 / Hit TAs4 / Length / Prob. beingEssential5 / Product
CJJ81176_0001 / rplC / 23 / 23 / 0 / 191 / 0.9458986 / 50S ribosomal protein L3
CJJ81176_0003 / - / 30 / 30 / 0 / 253 / 0.9857457 / RNA pseudouridine synthase family protein
CJJ81176_0005 / ksgA / 29 / 29 / 0 / 266 / 0.9827046 / dimethyladenosine transferase
CJJ81176_0025 / flgE / 83 / 83 / 0 / 838 / 0.9999996 / flagellar hook protein FlgE
CJJ81176_0027 / dnaA / 45 / 45 / 0 / 440 / 0.999241 / chromosomal replication initiation protein
CJJ81176_0050 / purB-1 / 41 / 41 / 0 / 442 / 0.9983364 / adenylosuccinate lyase
CJJ81176_0090 / mnmA / 39 / 39 / 0 / 338 / 0.9975379 / tRNA-specific 2-thiouridylase MnmA
CJJ81176_0104 / aroQ / 20 / 20 / 0 / 159 / 0.9065304 / 3-dehydroquinate dehydratase
CJJ81176_0131 / obgE / 33 / 33 / 0 / 356 / 0.9920427 / GTPase ObgE
CJJ81176_0133 / fmt / 36 / 36 / 0 / 305 / 0.9955705 / methionyl-tRNA formyltransferase
CJJ81176_0134 / - / 22 / 22 / 0 / 217 / 0.9349228 / biotin--protein ligase
CJJ81176_0139 / atpH / 24 / 24 / 0 / 173 / 0.9551122 / F0F1 ATP synthase subunit delta
CJJ81176_0140 / atpA / 51 / 51 / 0 / 501 / 0.9997663 / F0F1 ATP synthase subunit alpha
CJJ81176_0150 / slyD / 25 / 25 / 0 / 191 / 0.9628252 / FKBP-type peptidyl-prolyl cis-trans isomerase
CJJ81176_0151 / fabD / 21 / 21 / 0 / 306 / 0.921897 / malonyl CoA-acyl carrier protein transacylase
CJJ81176_0167 / lpxC / 35 / 35 / 0 / 294 / 0.9946139 / UDP-3-O-N-acetylglucosamine deacetylase
CJJ81176_0182 / trxB / 32 / 32 / 0 / 312 / 0.9903322 / thioredoxin-disulfide reductase
CJJ81176_0185 / hom / 35 / 35 / 0 / 415 / 0.994614 / homoserine dehydrogenase
CJJ81176_0187 / - / 42 / 42 / 0 / 268 / 0.9986327 / hypothetical protein
CJJ81176_0188 / - / 25 / 25 / 0 / 312 / 0.9628212 / hypothetical protein
CJJ81176_0201 / miaA / 34 / 34 / 0 / 289 / 0.9934527 / tRNA delta(2)-isopentenylpyrophosphate transferase
CJJ81176_0222 / def / 24 / 24 / 0 / 175 / 0.9551172 / peptide deformylase
CJJ81176_0237 / - / 38 / 38 / 0 / 267 / 0.9970051 / undecaprenol kinase, putative
CJJ81176_0255 / - / 51 / 51 / 0 / 456 / 0.9997663 / nicotinate phosphoribosyltransferase
CJJ81176_0256 / nrdF / 33 / 33 / 0 / 340 / 0.9920427 / ribonucleotide-diphosphate reductase
CJJ81176_0258 / pyrE / 22 / 22 / 0 / 202 / 0.934924 / orotate phosphoribosyltransferase
CJJ81176_0264 / - / 31 / 31 / 0 / 323 / 0.9882583 / NifU family protein
CJJ81176_0286 / pyrC / 40 / 40 / 0 / 335 / 0.9979761 / dihydroorotase
CJJ81176_0300 / fabZ / 20 / 20 / 0 / 146 / 0.9065373 / (3R)-hydroxymyristoyl-ACP dehydratase
CJJ81176_0301 / lpxA / 29 / 29 / 0 / 263 / 0.9827052 / UDP-N-acetylglucosamine acyltransferase
CJJ81176_0303 / mreB / 39 / 39 / 0 / 346 / 0.9975379 / rod shape-determining protein MreB
CJJ81176_0312 / - / 28 / 28 / 0 / 211 / 0.9790298 / hypothetical protein
CJJ81176_0330 / bioD / 23 / 23 / 0 / 201 / 0.9458994 / dethiobiotin synthetase
CJJ81176_0333 / rplY / 20 / 20 / 0 / 178 / 0.9065284 / 50S ribosomal protein L25/general stress protein
CJJ81176_0334 / pth / 24 / 24 / 0 / 181 / 0.9551093 / peptidyl-tRNA hydrolase
CJJ81176_0338 / pheA / 43 / 43 / 0 / 357 / 0.9988762 / chorismate mutase/prephenate dehydratase
CJJ81176_0341 / fliG / 43 / 43 / 0 / 342 / 0.9988762 / flagellar motor switch protein G
CJJ81176_0343 / dxs / 62 / 62 / 0 / 615 / 0.999973 / 1-deoxy-D-xylulose-5-phosphate synthase
CJJ81176_0350 / fabH / 36 / 36 / 0 / 324 / 0.9955701 / 3-oxoacyl-(acyl carrier protein) synthase III
CJJ81176_0377 / - / 45 / 45 / 0 / 486 / 0.999241 / Ppx/GppA family phosphatase
CJJ81176_0379 / - / 34 / 34 / 0 / 223 / 0.9934526 / DNA-binding response regulator
CJJ81176_0383 / glmM / 58 / 58 / 0 / 451 / 0.9999409 / phosphoglucosamine mutase
CJJ81176_0385 / - / 21 / 21 / 0 / 150 / 0.921898 / hypothetical protein
CJJ81176_0406 / ribH / 26 / 26 / 0 / 154 / 0.9692471 / 6,7-dimethyl-8-ribityllumazine synthase
CJJ81176_0407 / kdsA / 30 / 30 / 0 / 271 / 0.9857455 / 2-dehydro-3-deoxyphosphooctonate aldolase
CJJ81176_0411 / trpS / 33 / 33 / 0 / 319 / 0.9920429 / tryptophanyl-tRNA synthetase
CJJ81176_0424 / lysS / 56 / 56 / 0 / 501 / 0.9999124 / lysyl-tRNA synthetase
CJJ81176_0425 / glyA / 41 / 41 / 0 / 414 / 0.9983365 / serine hydroxymethyltransferase
CJJ81176_0429 / aroE / 37 / 37 / 0 / 262 / 0.9963574 / shikimate 5-dehydrogenase
CJJ81176_0458 / murD / 45 / 45 / 0 / 402 / 0.999241 / UDP-N-acetylmuramoyl-L-glutamatesynthetase
CJJ81176_0469 / fabF / 34 / 34 / 0 / 404 / 0.9934523 / 3-oxoacyl-(acyl carrier protein) synthase II
CJJ81176_0470 / accA / 34 / 34 / 0 / 312 / 0.9934526 / acetyl-CoA carboxylase carboxyltransferase
CJJ81176_0483 / miaB / 36 / 36 / 0 / 433 / 0.9955705 / adenosine tRNA methylthiotransferase
CJJ81176_0506 / rplA / 29 / 29 / 0 / 233 / 0.9827057 / 50S ribosomal protein L1
CJJ81176_0513 / fusA / 60 / 60 / 0 / 691 / 0.9999601 / elongation factor G
CJJ81176_0519 / trpC / 24 / 24 / 0 / 258 / 0.9551093 / indole-3-glycerol-phosphate synthase
CJJ81176_0532 / - / 39 / 39 / 0 / 288 / 0.9975378 / Gfo/Idh/MocA family oxidoreductase
CJJ81176_0534 / alaS / 82 / 82 / 0 / 842 / 0.9999995 / alanyl-tRNA synthetase
CJJ81176_0543 / - / 39 / 39 / 0 / 369 / 0.9975379 / hypothetical protein
CJJ81176_0552 / flgC / 28 / 28 / 0 / 164 / 0.9790304 / flagellar basal body rod protein FlgC
CJJ81176_0561 / oorA / 45 / 45 / 0 / 374 / 0.999241 / 2-oxoglutarate-acceptor oxidoreductase subunit
CJJ81176_0567 / hemA / 50 / 50 / 0 / 432 / 0.9997156 / glutamyl-tRNA reductase
CJJ81176_0576 / efp / 20 / 20 / 0 / 189 / 0.9065427 / elongation factor P
CJJ81176_0587 / dnaB / 56 / 56 / 0 / 458 / 0.9999124 / replicative DNA helicase
CJJ81176_0604 / lpxD / 43 / 43 / 0 / 321 / 0.9988762 / UDP-3-O-glucosamine N-acyltransferase
CJJ81176_0606 / tatC / 33 / 33 / 0 / 245 / 0.9920429 / Sec-independent protein translocase TatC
CJJ81176_0610 / - / 42 / 42 / 0 / 400 / 0.9986326 / aspartate kinase
CJJ81176_0614 / ligA / 61 / 61 / 0 / 647 / 0.9999672 / NAD-dependent DNA ligase LigA
CJJ81176_0624 / - / 24 / 24 / 0 / 273 / 0.9551161 / major antigenic peptide PEB4
CJJ81176_0625 / fbaA / 36 / 36 / 0 / 354 / 0.9955703 / fructose-bisphosphate aldolase
CJJ81176_0658 / - / 32 / 32 / 0 / 322 / 0.9903321 / DNA polymerase III subunit delta
CJJ81176_0664 / - / 30 / 30 / 0 / 279 / 0.9857456 / NOL1/NOP2/sun family protein
CJJ81176_0666 / ppa / 25 / 25 / 0 / 172 / 0.9628215 / inorganic pyrophosphatase
CJJ81176_0674 / rlpA / 25 / 25 / 0 / 266 / 0.9628186 / rare lipoprotein A
CJJ81176_0678 / engB / 21 / 21 / 0 / 198 / 0.9218947 / ribosome biogenesis GTP-binding protein YsxC
CJJ81176_0695 / - / 30 / 30 / 0 / 245 / 0.9857461 / ABC transporter, ATP-binding protein
CJJ81176_0715 / - / 33 / 33 / 0 / 312 / 0.9920424 / hypothetical protein
CJJ81176_0716 / mraW / 29 / 29 / 0 / 310 / 0.9827052 / S-adenosyl-methyltransferase MraW
CJJ81176_0722 / glnA / 61 / 61 / 0 / 476 / 0.9999672 / glutamine synthetase, type I
CJJ81176_0727 / glyQ / 24 / 24 / 0 / 287 / 0.9551141 / glycyl-tRNA synthetase subunit alpha
CJJ81176_0729 / - / 33 / 33 / 0 / 238 / 0.9920426 / hypothetical protein
CJJ81176_0739 / - / 36 / 36 / 0 / 446 / 0.9955702 / 3-deoxy-7-phosphoheptulonate synthase
CJJ81176_0773 / hrcA / 31 / 31 / 0 / 263 / 0.9882585 / heat-inducible transcription repressor
CJJ81176_0775 / dnaK / 63 / 63 / 0 / 623 / 0.9999778 / molecular chaperone DnaK
CJJ81176_0820 / ruvA / 20 / 20 / 0 / 183 / 0.9065337 / Holliday junction DNA helicase RuvA
CJJ81176_0823 / cysS / 60 / 60 / 0 / 462 / 0.9999601 / cysteinyl-tRNA synthetase
CJJ81176_0833 / thrC / 43 / 43 / 0 / 470 / 0.9988762 / threonine synthase
CJJ81176_0834 / kdsB / 28 / 28 / 0 / 239 / 0.9790311 / 3-deoxy-manno-octulosonate cytidylyltransferase
CJJ81176_0838 / glmU / 37 / 37 / 0 / 429 / 0.9963575 / N-acetylglucosamine-1-phosphate uridyltransferase
CJJ81176_0839 / coaBC / 37 / 37 / 0 / 384 / 0.9963575 / phosphopantothenoylcysteine decarboxylase
CJJ81176_0857 / fbp / 28 / 28 / 0 / 280 / 0.9790303 / fructose-1,6-bisphosphatase
CJJ81176_0863 / psd / 32 / 32 / 0 / 266 / 0.9903322 / phosphatidylserine decarboxylase
CJJ81176_0871 / folD / 22 / 22 / 0 / 282 / 0.9349177 / 5,10-methylene-tetrahydrofolate dehydrogenase
CJJ81176_0874 / murA / 49 / 49 / 0 / 418 / 0.9996539 / UDP-N-acetylglucosamine 1-carboxyvinyltransferase
CJJ81176_0903 / ispH / 29 / 29 / 0 / 277 / 0.9827052 / 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
CJJ81176_0914 / alr / 38 / 38 / 0 / 328 / 0.9970051 / alanine racemase
CJJ81176_0920 / cysK / 34 / 34 / 0 / 253 / 0.9934528 / cysteine synthase A
CJJ81176_0925 / prsA / 29 / 29 / 0 / 309 / 0.9827041 / ribose-phosphate pyrophosphokinase
CJJ81176_0934 / apt / 25 / 25 / 0 / 182 / 0.9628221 / adenine phosphoribosyltransferase
CJJ81176_0973 / - / 35 / 35 / 0 / 325 / 0.994614 / peptidyl-arginine deiminase family protein
CJJ81176_0977 / - / 21 / 21 / 0 / 212 / 0.9219115 / DnaJ domain-containing protein
CJJ81176_0980 / - / 22 / 22 / 0 / 272 / 0.934921 / hypothetical protein
CJJ81176_0981 / - / 56 / 56 / 0 / 528 / 0.9999124 / translocase component YidC
CJJ81176_1013 / hemB / 34 / 34 / 0 / 327 / 0.9934524 / delta-aminolevulinic acid dehydratase
CJJ81176_1035 / livM / 43 / 43 / 0 / 350 / 0.9988762 / branched-chain amino acid ABC transporter
CJJ81176_1067 / thiF / 28 / 28 / 0 / 267 / 0.9790306 / thiamine biosynthesis protein ThiF
CJJ81176_1074 / murC / 53 / 53 / 0 / 431 / 0.9998422 / UDP-N-acetylmuramate--L-alanine ligase
CJJ81176_1078 / guaB / 55 / 55 / 0 / 485 / 0.9998934 / inosine 5'-monophosphate dehydrogenase
CJJ81176_1098 / hemD / 28 / 28 / 0 / 209 / 0.9790295 / uroporphyrinogen-III synthase
CJJ81176_1122 / ispE / 26 / 26 / 0 / 255 / 0.969247 / 4-diphosphocytidyl-D-erythritol kinase
CJJ81176_1140 / pglD / 25 / 25 / 0 / 203 / 0.9628221 / general glycosylation pathway protein
CJJ81176_1150 / waaC / 37 / 37 / 0 / 342 / 0.9963573 / lipopolysaccharide heptosyltransferase I
CJJ81176_1164 / waaF / 38 / 38 / 0 / 317 / 0.997005 / ADP-heptose--LPS heptosyltransferase II
CJJ81176_1167 / hldE / 42 / 42 / 0 / 461 / 0.9986327 / D,D-heptose 1-phosphate adenosyltransferase
CJJ81176_1168 / waaD / 32 / 32 / 0 / 317 / 0.990332 / ADP-L-glycero-D-mannoheptose-6-epimerase
CJJ81176_1195 / - / 23 / 23 / 0 / 211 / 0.9459001 / ABC transporter, ATP-binding protein
CJJ81176_1196 / tsf / 30 / 30 / 0 / 357 / 0.9857458 / elongation factor Ts
CJJ81176_1200 / petB / 43 / 43 / 0 / 416 / 0.9988762 / ubiquinol--cytochrome c reductase
CJJ81176_1204 / - / 22 / 22 / 0 / 165 / 0.9349246 / methyl-accepting chemotaxis protein
CJJ81176_1211 / gpsA / 31 / 31 / 0 / 297 / 0.9882574 / glycerol-3-phosphate dehydrogenase
CJJ81176_1221 / ftsY / 29 / 29 / 0 / 288 / 0.9827051 / signal recognition particle-docking protein
CJJ81176_1222 / - / 20 / 20 / 0 / 185 / 0.9065391 / putative lipoprotein
CJJ81176_1226 / glcD / 46 / 46 / 0 / 460 / 0.9993763 / glycolate oxidase, subunit GlcD
CJJ81176_1227 / - / 31 / 31 / 0 / 236 / 0.9882579 / hypothetical protein
CJJ81176_1231 / ribE / 25 / 25 / 0 / 203 / 0.9628217 / riboflavin synthase subunit alpha
CJJ81176_1241 / - / 36 / 36 / 0 / 224 / 0.9955703 / DNA-binding response regulator
CJJ81176_1243 / dnaJ-1 / 30 / 30 / 0 / 297 / 0.9857452 / co-chaperone protein DnaJ
CJJ81176_1252 / pdxJ / 29 / 29 / 0 / 262 / 0.9827049 / pyridoxine 5'-phosphate synthase
CJJ81176_1262 / - / 26 / 26 / 0 / 164 / 0.9692501 / hypothetical protein
CJJ81176_1266 / purD / 38 / 38 / 0 / 416 / 0.9970051 / phosphoribosylamine--glycine ligase
CJJ81176_1268 / - / 62 / 62 / 0 / 675 / 0.999973 / organic solvent tolerance protein, putative
CJJ81176_1275 / porA / 41 / 41 / 0 / 424 / 0.9983364 / major outer membrane protein
CJJ81176_1277 / racR / 34 / 34 / 0 / 223 / 0.9934526 / DNA-binding response regulator
CJJ81176_1285 / - / 73 / 73 / 0 / 659 / 0.9999969 / N-acetylmuramoyl-L-alanine amidase
CJJ81176_1293 / lolD / 27 / 27 / 0 / 221 / 0.9745933 / lipoprotein releasing system ATP-binding protein
CJJ81176_1296 / - / 38 / 38 / 0 / 322 / 0.9970051 / ribosomal large subunit pseudouridine synthase A
CJJ81176_1306 / - / 36 / 36 / 0 / 271 / 0.9955701 / hypothetical protein
CJJ81176_1311 / - / 40 / 40 / 0 / 376 / 0.9979761 / aminotransferase
CJJ81176_1329 / - / 28 / 28 / 0 / 274 / 0.9790296 / flagellar protein, putative
CJJ81176_1334 / neuB / 33 / 33 / 0 / 343 / 0.9920429 / N-acetylneuraminic acid synthetase
CJJ81176_1345 / dxr / 34 / 34 / 0 / 356 / 0.9934526 / 1-deoxy-D-xylulose 5-phosphate reductoisomerase
CJJ81176_1346 / cdsA / 35 / 35 / 0 / 241 / 0.9946139 / phosphatidate cytidylyltransferase
CJJ81176_1370 / - / 41 / 41 / 0 / 354 / 0.9983364 / hypothetical protein
CJJ81176_1384 / fldA / 22 / 22 / 0 / 163 / 0.9349244 / flavodoxin FldA
CJJ81176_1401 / pgk / 55 / 55 / 0 / 400 / 0.9998934 / phosphoglycerate kinase
CJJ81176_1402 / gapA / 42 / 42 / 0 / 332 / 0.9986327 / glyceraldehyde 3-phosphate dehydrogenase A
CJJ81176_1403 / nadD / 25 / 25 / 0 / 181 / 0.9628215 / nicotinate nucleotide adenylyltransferase
CJJ81176_1406 / algC / 56 / 56 / 0 / 456 / 0.9999124 / phosphomannomutase/phosphoglucomutase
CJJ81176_1422 / - / 24 / 24 / 0 / 224 / 0.9551158 / capsular biosynthesis nucleotidyltransferase
CJJ81176_1437 / kpsF / 34 / 34 / 0 / 315 / 0.9934526 / arabinose-5-phosphate isomerase
CJJ81176_1438 / kpsD / 65 / 65 / 0 / 552 / 0.999985 / periplasmic polysaccharide-binding protein
CJJ81176_1441 / kpsM / 37 / 37 / 0 / 260 / 0.9963574 / permease protein
CJJ81176_1446 / tilS / 31 / 31 / 0 / 321 / 0.988258 / tRNA(Ile)-lysidine synthetase
CJJ81176_1455 / flgI / 38 / 38 / 0 / 348 / 0.9970051 / flagellar basal body P-ring protein
CJJ81176_1477 / - / 21 / 21 / 0 / 200 / 0.9218946 / hypothetical protein
CJJ81176_1479 / ccoP / 36 / 36 / 0 / 287 / 0.9955703 / cytochrome c oxidase, cbb3-type, subunit III
CJJ81176_1490 / purA / 48 / 48 / 0 / 416 / 0.9995788 / adenylosuccinate synthetase
CJJ81176_1507 / nspC / 34 / 34 / 0 / 382 / 0.9934528 / carboxynorspermidine decarboxylase
CJJ81176_1515 / coaE / 21 / 21 / 0 / 201 / 0.9219099 / dephospho-CoA kinase
CJJ81176_1520 / pgi / 51 / 51 / 0 / 406 / 0.9997663 / glucose-6-phosphate isomerase
CJJ81176_1521 / galU / 26 / 26 / 0 / 274 / 0.9692482 / UTP-glucose-1-phosphate uridylyltransferase
CJJ81176_1551 / nuoN / 55 / 55 / 0 / 462 / 0.9998934 / NADH-quinone oxidoreductase, N subunit
CJJ81176_1582 / rpoA / 41 / 41 / 0 / 337 / 0.9983365 / DNA-directed RNA polymerase subunit alpha
CJJ81176_1595 / - / 45 / 45 / 0 / 295 / 0.999241 / DNA-binding response regulator, putative
CJJ81176_1625 / aroC / 31 / 31 / 0 / 362 / 0.9882582 / chorismate synthase
CJJ81176_1635 / ispA / 23 / 23 / 0 / 281 / 0.9458987 / geranyltranstransferase
CJJ81176_1636 / tkt / 54 / 54 / 0 / 632 / 0.9998703 / transketolase
CJJ81176_1668 / eno / 36 / 36 / 0 / 414 / 0.9955703 / phosphopyruvate hydratase
CJJ81176_1670 / - / 43 / 43 / 0 / 286 / 0.9988762 / hypothetical protein
CJJ81176_1677 / bioB / 26 / 26 / 0 / 278 / 0.9692492 / biotin synthase
CJJ81176_1692 / rplE / 27 / 27 / 0 / 181 / 0.9745925 / 50S ribosomal protein L5
CJJ81176_1698 / rpsC / 23 / 23 / 0 / 233 / 0.9458999 / 30S ribosomal protein S3
CJJ81176_1699 / rplV / 20 / 20 / 0 / 147 / 0.9065294 / 50S ribosomal protein L22
CJJ81176_1701 / rplB / 33 / 33 / 0 / 276 / 0.9920426 / 50S ribosomal protein L2
CJJ81176_1703 / rplD / 25 / 25 / 0 / 204 / 0.9628196 / 50S ribosomal protein L4
1: For criteria of essential gene analysis, see Methods section.
2: total number of insertion sites in each gene; C-terminal 20% excluded;
3:number of unique insertion sites in each gene;
4: number of unique insertion sites in each gene disrupted by transposons in the mutagenized input population;
5: probability that each gene is essential